MYB35_ARATH
ID MYB35_ARATH Reviewed; 317 AA.
AC Q9LSI7; A0MEZ1; O49770;
DT 22-JUL-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Transcription factor MYB35 {ECO:0000303|PubMed:9839469};
DE AltName: Full=Myb-related protein 35 {ECO:0000303|PubMed:9839469};
DE Short=AtMYB35 {ECO:0000303|PubMed:9839469};
DE AltName: Full=Protein DEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION 1 {ECO:0000303|PubMed:18397379};
GN Name=MYB35 {ECO:0000303|PubMed:9839469};
GN Synonyms=TDF1 {ECO:0000303|PubMed:18397379};
GN OrderedLocusNames=At3g28470 {ECO:0000312|Araport:AT3G28470};
GN ORFNames=MFJ20.16 {ECO:0000312|EMBL:BAB02863.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND GENE FAMILY.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 54-290, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=9839469; DOI=10.1046/j.1365-313x.1998.00278.x;
RA Kranz H.D., Denekamp M., Greco R., Jin H.-L., Leyva A., Meissner R.C.,
RA Petroni K., Urzainqui A., Bevan M., Martin C., Smeekens S., Tonelli C.,
RA Paz-Ares J., Weisshaar B.;
RT "Towards functional characterisation of the members of the R2R3-MYB gene
RT family from Arabidopsis thaliana.";
RL Plant J. 16:263-276(1998).
RN [6]
RP GENE FAMILY.
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=17666023; DOI=10.1111/j.1365-313x.2007.03217.x;
RA Wijeratne A.J., Zhang W., Sun Y., Liu W., Albert R., Zheng Z.,
RA Oppenheimer D.G., Zhao D., Ma H.;
RT "Differential gene expression in Arabidopsis wild-type and mutant anthers:
RT insights into anther cell differentiation and regulatory networks.";
RL Plant J. 52:14-29(2007).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=18397379; DOI=10.1111/j.1365-313x.2008.03500.x;
RA Zhu J., Chen H., Li H., Gao J.F., Jiang H., Wang C., Guan Y.F., Yang Z.N.;
RT "Defective in Tapetal development and function 1 is essential for anther
RT development and tapetal function for microspore maturation in
RT Arabidopsis.";
RL Plant J. 55:266-277(2008).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=21957980; DOI=10.1111/j.1744-7909.2011.01078.x;
RA Zhu J., Lou Y., Xu X., Yang Z.N.;
RT "A genetic pathway for tapetum development and function in Arabidopsis.";
RL J. Integr. Plant Biol. 53:892-900(2011).
CC -!- FUNCTION: Required for anther development and early tapetal function
CC during microspore maturation (PubMed:18397379, PubMed:21957980).
CC Regulates callose dissolution required for microspores release from the
CC tetrads (PubMed:18397379). {ECO:0000269|PubMed:18397379,
CC ECO:0000269|PubMed:21957980}.
CC -!- INTERACTION:
CC Q9LSI7; Q9SYG2: ASIL1; NbExp=3; IntAct=EBI-25523050, EBI-4424669;
CC Q9LSI7; O80450: GT-3B; NbExp=3; IntAct=EBI-25523050, EBI-1571089;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:18397379}.
CC -!- TISSUE SPECIFICITY: Inflorescences-specific (PubMed:18397379).
CC Accumulates in anthers, especially in tapetum and
CC meiocytes/microsporocytes and microspores during anther development
CC (PubMed:17666023, PubMed:18397379). {ECO:0000269|PubMed:17666023,
CC ECO:0000269|PubMed:18397379}.
CC -!- DEVELOPMENTAL STAGE: During anther development, first confined to
CC meiocytes, tapetal and middle layer cells. At the microspore stage,
CC mainly expressed in the tapetum and microspores. Later observed in
CC developing pollen grains. {ECO:0000269|PubMed:18397379,
CC ECO:0000269|PubMed:21957980}.
CC -!- DISRUPTION PHENOTYPE: Male-sterile mutant, defective in tapetal
CC development, characterized by irregular and excessive division,
CC redundant cells and leading to dysfunction of the tapetum
CC (PubMed:18397379, PubMed:21957980). Impaired callose dissolution
CC resulting in altered microspores release from the tetrads and no pollen
CC production (PubMed:18397379). {ECO:0000269|PubMed:18397379,
CC ECO:0000269|PubMed:21957980}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC83599.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=ABK28578.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB026644; BAB02863.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77449.1; -; Genomic_DNA.
DR EMBL; DQ446711; ABE65977.1; -; mRNA.
DR EMBL; DQ653114; ABK28578.1; ALT_SEQ; mRNA.
DR EMBL; AY519591; AAS10061.1; -; mRNA.
DR EMBL; AF062877; AAC83599.1; ALT_FRAME; mRNA.
DR PIR; T51649; T51649.
DR RefSeq; NP_189488.1; NM_113767.2.
DR AlphaFoldDB; Q9LSI7; -.
DR SMR; Q9LSI7; -.
DR IntAct; Q9LSI7; 2.
DR STRING; 3702.AT3G28470.1; -.
DR PaxDb; Q9LSI7; -.
DR PRIDE; Q9LSI7; -.
DR EnsemblPlants; AT3G28470.1; AT3G28470.1; AT3G28470.
DR GeneID; 822477; -.
DR Gramene; AT3G28470.1; AT3G28470.1; AT3G28470.
DR KEGG; ath:AT3G28470; -.
DR Araport; AT3G28470; -.
DR TAIR; locus:2088957; AT3G28470.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_15_2_1; -.
DR InParanoid; Q9LSI7; -.
DR OMA; VANHGTG; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q9LSI7; -.
DR PRO; PR:Q9LSI7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LSI7; baseline and differential.
DR Genevisible; Q9LSI7; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0048658; P:anther wall tapetum development; IMP:UniProtKB.
DR GO; GO:0052545; P:callose localization; IMP:UniProtKB.
DR GO; GO:0055046; P:microgametogenesis; IMP:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation.
FT CHAIN 1..317
FT /note="Transcription factor MYB35"
FT /id="PRO_0000433487"
FT DOMAIN 9..65
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 66..116
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 37..61
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 89..112
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT CONFLICT 234
FT /note="T -> A (in Ref. 5; AAC83599)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 317 AA; 35813 MW; A850824AE22CC2B1 CRC64;
MGRPPCCDKS NVKKGLWTEE EDAKILAYVA IHGVGNWSLI PKKAGLNRCG KSCRLRWTNY
LRPDLKHDSF STQEEELIIE CHRAIGSRWS SIARKLPGRT DNDVKNHWNT KLKKKLMKMG
IDPVTHKPVS QLLAEFRNIS GHGNASFKTE PSNNSILTQS NSAWEMMRNT TTNHESYYTN
SPMMFTNSSE YQTTPFHFYS HPNHLLNGTT SSCSSSSSST SITQPNQVPQ TPVTNFYWSD
FLLSDPVPQV VGSSATSDLT FTQNEHHFNI EAEYISQNID SKASGTCHSA SSFVDEILDK
DQEMLSQFPQ LLNDFDY
