MYB44_ARATH
ID MYB44_ARATH Reviewed; 305 AA.
AC Q9FDW1; Q39154; Q8LBC5; Q941B3; Q9SAM4;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Transcription factor MYB44 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein 44 {ECO:0000303|PubMed:11597504};
DE Short=AtMYB44 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein R1 {ECO:0000303|PubMed:9678577};
DE Short=AtMYBR1 {ECO:0000303|PubMed:9678577};
GN Name=MYB44 {ECO:0000303|PubMed:11597504};
GN Synonyms=MYBR1 {ECO:0000303|PubMed:9678577};
GN OrderedLocusNames=At5g67300 {ECO:0000312|Araport:AT5G67300};
GN ORFNames=K8K14.2 {ECO:0000312|EMBL:BAB09015.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Landsberg erecta; TISSUE=Silique;
RX PubMed=9678577; DOI=10.1023/a:1006011002499;
RA Kirik V., Koelle K., Misera S., Baeumlein H.;
RT "Two novel MYB homologues with changed expression in late embryogenesis-
RT defective Arabidopsis mutants.";
RL Plant Mol. Biol. 37:819-827(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L.-J., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 46-90.
RC STRAIN=cv. Landsberg erecta;
RA Romero I., Fuertes A., Benito M.J., Malpica J., Leyva A., Paz-Ares J.;
RT "One hundred R2R3-MYB genes in the genome of Arabidopsis thaliana.";
RL Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [9]
RP GENE FAMILY, AND INDUCTION.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [10]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=18162593; DOI=10.1104/pp.107.110981;
RA Jung C., Seo J.S., Han S.W., Koo Y.J., Kim C.H., Song S.I., Nahm B.H.,
RA Choi Y.D., Cheong J.-J.;
RT "Overexpression of AtMYB44 enhances stomatal closure to confer abiotic
RT stress tolerance in transgenic Arabidopsis.";
RL Plant Physiol. 146:623-635(2008).
RN [11]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION BY JASMONATE AND SALICYLIC
RP ACID.
RC STRAIN=cv. Columbia;
RX PubMed=23067202; DOI=10.1111/tpj.12051;
RA Shim J.S., Jung C., Lee S., Min K., Lee Y.-W., Choi Y., Lee J.S.,
RA Song J.T., Kim J.-K., Choi Y.D.;
RT "AtMYB44 regulates WRKY70 expression and modulates antagonistic interaction
RT between salicylic acid and jasmonic acid signaling.";
RL Plant J. 73:483-495(2013).
RN [12]
RP FUNCTION.
RX PubMed=23603962; DOI=10.4161/psb.24509;
RA Shim J.S., Choi Y.D.;
RT "Direct regulation of WRKY70 by AtMYB44 in plant defense responses.";
RL Plant Signal. Behav. 8:E20783-E20783(2013).
RN [13]
RP FUNCTION, AND INTERACTION WITH PYL8.
RX PubMed=24894996; DOI=10.1126/scisignal.2005051;
RA Zhao Y., Xing L., Wang X., Hou Y.J., Gao J., Wang P., Duan C.G., Zhu X.,
RA Zhu J.K.;
RT "The ABA receptor PYL8 promotes lateral root growth by enhancing MYB77-
RT dependent transcription of auxin-responsive genes.";
RL Sci. Signal. 7:RA53-RA53(2014).
CC -!- FUNCTION: Transcription factor (PubMed:23067202, PubMed:23603962).
CC Represses the expression of protein phosphatases 2C in response to
CC abscisic acid (ABA). Confers resistance to abiotic stresses dependent
CC of ABA (PubMed:18162593, PubMed:9678577). In response to auxin,
CC activates the transcription of the auxin-responsive gene IAA19. The
CC IAA19 transcription activation by MYB44 is enhanced by direct
CC interaction between MYB44 and PYL8 (PubMed:24894996). Transcriptional
CC activator of WRKY70 by direct binding to its promoter region,
CC especially at 5'-TAACNG-3' and 5'-CNGTTA-3' symmetric motifs
CC (PubMed:23067202, PubMed:23603962). Activates salicylic acid
CC (SA)- mediated defenses and subsequent resistance to biotrophic
CC pathogen P.syringae pv. tomato DC3000, but represses jasmonic acid
CC (JA)-mediated defenses responses against the necrotrophic pathogen
CC A.brassicicola (PubMed:23067202). {ECO:0000269|PubMed:18162593,
CC ECO:0000269|PubMed:23067202, ECO:0000269|PubMed:23603962,
CC ECO:0000269|PubMed:24894996, ECO:0000269|PubMed:9678577}.
CC -!- SUBUNIT: Interacts with PYL8. {ECO:0000269|PubMed:24894996}.
CC -!- INTERACTION:
CC Q9FDW1; Q8L9Y3: ARR14; NbExp=3; IntAct=EBI-15192813, EBI-1100737;
CC Q9FDW1; Q9FMP4: At5g12190; NbExp=3; IntAct=EBI-15192813, EBI-4459215;
CC Q9FDW1; Q9SGS4: CDSP32; NbExp=3; IntAct=EBI-15192813, EBI-25517863;
CC Q9FDW1; Q8LPI7: CKL4; NbExp=3; IntAct=EBI-15192813, EBI-25516910;
CC Q9FDW1; Q9SFV2: FHA2; NbExp=3; IntAct=EBI-15192813, EBI-15191747;
CC Q9FDW1; Q84JZ6: MORF3; NbExp=3; IntAct=EBI-15192813, EBI-4465639;
CC Q9FDW1; Q9FDW1: MYB44; NbExp=7; IntAct=EBI-15192813, EBI-15192813;
CC Q9FDW1; O22179: MYB70; NbExp=5; IntAct=EBI-15192813, EBI-1238013;
CC Q9FDW1; O23160: MYB73; NbExp=5; IntAct=EBI-15192813, EBI-25506855;
CC Q9FDW1; Q9FGM1: PYL8; NbExp=5; IntAct=EBI-15192813, EBI-2429535;
CC Q9FDW1; Q9XGN1: TTG1; NbExp=3; IntAct=EBI-15192813, EBI-395803;
CC Q9FDW1; Q9XFB1: YAB3; NbExp=3; IntAct=EBI-15192813, EBI-1115657;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:18162593}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, inflorescence,
CC and flowers (including stamen, floral nectar, carpel, petal and sepal),
CC mostly in vasculatures and stomata. {ECO:0000269|PubMed:18162593,
CC ECO:0000269|PubMed:9678577}.
CC -!- DEVELOPMENTAL STAGE: Expressed during very late stages of
CC embryogenesis. Later, its expression follows a development dependent
CC gradient in successive leaves. {ECO:0000269|PubMed:9678577}.
CC -!- INDUCTION: By drought, cold, high salinity, cadmium (CdCl(2)),
CC salicylic acid (SA), jasmonate (JA), ethylene, gibberellic acid (GA),
CC and ABA (PubMed:16463103, PubMed:18162593, PubMed:23067202). The
CC induction by JA is COI1-dependent (PubMed:23067202).
CC {ECO:0000269|PubMed:16463103, ECO:0000269|PubMed:18162593,
CC ECO:0000269|PubMed:23067202}.
CC -!- DISRUPTION PHENOTYPE: Increased defense responses against the
CC necrotrophic pathogen A.brassicicola. Down-regulation of salicylic acid
CC (SA)- mediated WRKY70 and PR genes expression, leading to reduced
CC resistance to the biotrophic pathogen P.syringae pv. tomato DC3000.
CC {ECO:0000269|PubMed:23067202}.
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DR EMBL; Z54136; CAA90809.1; -; mRNA.
DR EMBL; AY519648; AAS10118.1; -; mRNA.
DR EMBL; AB007645; BAB09015.1; -; Genomic_DNA.
DR EMBL; CP002688; AED98326.1; -; Genomic_DNA.
DR EMBL; AF326877; AAG41459.1; -; mRNA.
DR EMBL; AF339698; AAK00380.1; -; mRNA.
DR EMBL; AY052297; AAK96490.1; -; mRNA.
DR EMBL; AY061923; AAL31250.1; -; mRNA.
DR EMBL; AY087295; AAM64847.1; -; mRNA.
DR EMBL; Z95768; CAB09200.1; -; mRNA.
DR PIR; S71284; S71284.
DR RefSeq; NP_201531.1; NM_126130.2.
DR AlphaFoldDB; Q9FDW1; -.
DR SMR; Q9FDW1; -.
DR BioGRID; 22107; 28.
DR IntAct; Q9FDW1; 24.
DR STRING; 3702.AT5G67300.1; -.
DR iPTMnet; Q9FDW1; -.
DR PaxDb; Q9FDW1; -.
DR PRIDE; Q9FDW1; -.
DR ProteomicsDB; 251273; -.
DR EnsemblPlants; AT5G67300.1; AT5G67300.1; AT5G67300.
DR GeneID; 836865; -.
DR Gramene; AT5G67300.1; AT5G67300.1; AT5G67300.
DR KEGG; ath:AT5G67300; -.
DR Araport; AT5G67300; -.
DR TAIR; locus:2158212; AT5G67300.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_14_0_1; -.
DR InParanoid; Q9FDW1; -.
DR OMA; ESECDRI; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q9FDW1; -.
DR PRO; PR:Q9FDW1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FDW1; baseline and differential.
DR Genevisible; Q9FDW1; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IEP:TAIR.
DR GO; GO:0010929; P:positive regulation of auxin mediated signaling pathway; IDA:UniProtKB.
DR GO; GO:1900150; P:regulation of defense response to fungus; IMP:UniProtKB.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:2000031; P:regulation of salicylic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0009737; P:response to abscisic acid; IMP:TAIR.
DR GO; GO:0009408; P:response to heat; IEP:TAIR.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:UniProtKB.
DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB.
DR GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IEP:TAIR.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW Abscisic acid signaling pathway; Activator; DNA-binding;
KW Jasmonic acid signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..305
FT /note="Transcription factor MYB44"
FT /id="PRO_0000344526"
FT DOMAIN 1..56
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 58..107
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 29..52
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 80..103
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 116..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 180..229
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 186
FT /note="S -> F (in Ref. 1; CAA90809)"
FT /evidence="ECO:0000305"
FT CONFLICT 261
FT /note="V -> L (in Ref. 5; AAL31250/AAK96490)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 305 AA; 33268 MW; FDD31F9916E14D9D CRC64;
MADRIKGPWS PEEDEQLRRL VVKYGPRNWT VISKSIPGRS GKSCRLRWCN QLSPQVEHRP
FSAEEDETIA RAHAQFGNKW ATIARLLNGR TDNAVKNHWN STLKRKCGGY DHRGYDGSED
HRPVKRSVSA GSPPVVTGLY MSPGSPTGSD VSDSSTIPIL PSVELFKPVP RPGAVVLPLP
IETSSSSDDP PTSLSLSLPG ADVSEESNRS HESTNINNTT SSRHNHNNTV SFMPFSGGFR
GAIEEMGKSF PGNGGEFMAV VQEMIKAEVR SYMTEMQRNN GGGFVGGFID NGMIPMSQIG
VGRIE
