MYB46_ARATH
ID MYB46_ARATH Reviewed; 280 AA.
AC Q9LXV2; Q9ZTE1;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Transcription factor MYB46 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein 46 {ECO:0000303|PubMed:11597504};
DE Short=AtMYB46 {ECO:0000303|PubMed:11597504};
GN Name=MYB46 {ECO:0000303|PubMed:11597504}; OrderedLocusNames=At5g12870;
GN ORFNames=T24H18.40;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 75-280, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=9839469; DOI=10.1046/j.1365-313x.1998.00278.x;
RA Kranz H.D., Denekamp M., Greco R., Jin H.-L., Leyva A., Meissner R.C.,
RA Petroni K., Urzainqui A., Bevan M., Martin C., Smeekens S., Tonelli C.,
RA Paz-Ares J., Weisshaar B.;
RT "Towards functional characterisation of the members of the R2R3-MYB gene
RT family from Arabidopsis thaliana.";
RL Plant J. 16:263-276(1998).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [7]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16581911; DOI=10.1073/pnas.0510607103;
RA Lee J.-Y., Colinas J., Wang J.Y., Mace D., Ohler U., Benfey P.N.;
RT "Transcriptional and posttranscriptional regulation of transcription factor
RT expression in Arabidopsis roots.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:6055-6060(2006).
RN [8]
RP INDUCTION BY SA, AND GENE FAMILY.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [9]
RP FUNCTION, INDUCTION BY SND1, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=17890373; DOI=10.1105/tpc.107.053678;
RA Zhong R., Richardson E.A., Ye Z.-H.;
RT "The MYB46 transcription factor is a direct target of SND1 and regulates
RT secondary wall biosynthesis in Arabidopsis.";
RL Plant Cell 19:2776-2792(2007).
RN [10]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19808805; DOI=10.1093/pcp/pcp139;
RA McCarthy R.L., Zhong R., Ye Z.-H.;
RT "MYB83 is a direct target of SND1 and acts redundantly with MYB46 in the
RT regulation of secondary cell wall biosynthesis in Arabidopsis.";
RL Plant Cell Physiol. 50:1950-1964(2009).
RN [11]
RP FUNCTION.
RX PubMed=22197883; DOI=10.1093/pcp/pcr185;
RA Zhong R., Ye Z.H.;
RT "MYB46 and MYB83 bind to the SMRE sites and directly activate a suite of
RT transcription factors and secondary wall biosynthetic genes.";
RL Plant Cell Physiol. 53:368-380(2012).
RN [12]
RP FUNCTION.
RX PubMed=23726771; DOI=10.1016/j.jplph.2013.04.012;
RA Kim W.C., Kim J.Y., Ko J.H., Kim J., Han K.H.;
RT "Transcription factor MYB46 is an obligate component of the transcriptional
RT regulatory complex for functional expression of secondary wall-associated
RT cellulose synthases in Arabidopsis thaliana.";
RL J. Plant Physiol. 170:1374-1378(2013).
RN [13]
RP FUNCTION.
RX PubMed=24243147; DOI=10.1007/s11103-013-0154-9;
RA Kim W.C., Reca I.B., Kim Y., Park S., Thomashow M.F., Keegstra K.,
RA Han K.H.;
RT "Transcription factors that directly regulate the expression of CSLA9
RT encoding mannan synthase in Arabidopsis thaliana.";
RL Plant Mol. Biol. 84:577-587(2014).
CC -!- FUNCTION: Transcription activator. Involved in the regulation of
CC secondary wall biosynthesis in fibers and vessels (PubMed:17890373).
CC Transcription activator of the mannan synthase CSLA9 that recognizes
CC and binds to the DNA consensus sequence 5'-[AG][GT]T[AT]GGT[GA]-3' cis-
CC regulatory element of CSLA9 promoter (PubMed:24243147). Transcription
CC factor that acts as molecular switch in the NAC012/SND1-mediated
CC transcriptional network regulating secondary wall biosynthesis. Is
CC directly activated by NAC012/SND1. Functions redundantly with MYB83 in
CC the transcriptional regulatory cascade leading to secondary wall
CC formation in fibers and vessels (PubMed:19808805). Transcription
CC activator that binds to the DNA consensus sequence 5'-ACC[AT]A[AC][TC]-
CC 3', designated as the secondary wall MYB-responsive element (SMRE).
CC Regulates directly numerous transcription factors and a number of genes
CC involved in secondary wall biosynthesis that contain SMRE elements in
CC their promoters (PubMed:22197883). Is an obligate component of the
CC transcriptional regulatory complex toward the commitment of secondary
CC wall cellulose synthesis. Is required for functional expression of the
CC three secondary wall CESA genes, CESA4, CESA7 and CESA8
CC (PubMed:23726771). {ECO:0000269|PubMed:17890373,
CC ECO:0000269|PubMed:19808805, ECO:0000269|PubMed:22197883,
CC ECO:0000269|PubMed:23726771, ECO:0000269|PubMed:24243147}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:16581911, ECO:0000269|PubMed:17890373}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in stems and siliques,
CC specifically in xylem. {ECO:0000269|PubMed:16581911,
CC ECO:0000269|PubMed:17890373, ECO:0000269|PubMed:9839469}.
CC -!- INDUCTION: Slightly induced by salicylic acid (SA). Positively
CC regulated by SND1 and homolog proteins. {ECO:0000269|PubMed:16463103,
CC ECO:0000269|PubMed:17890373}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but the double mutant plants myb48 and myb83 nearly lack
CC secondary wall thickening, stop growing after developing one to two
CC pairs of small leaves and subsequently die.
CC {ECO:0000269|PubMed:19808805}.
CC -!- MISCELLANEOUS: Plants over-expressing MYB46 show a significant increase
CC in mannan synthase activity and mannan content in plant.
CC {ECO:0000269|PubMed:24243147}.
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DR EMBL; AY519621; AAS10091.1; -; mRNA.
DR EMBL; AL353013; CAB88251.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91824.1; -; Genomic_DNA.
DR EMBL; BT000455; AAN17432.1; -; mRNA.
DR EMBL; BT002549; AAO00909.1; -; mRNA.
DR EMBL; AF062884; AAC83606.1; -; mRNA.
DR PIR; T49901; T49901.
DR PIR; T51656; T51656.
DR RefSeq; NP_196791.1; NM_121290.3.
DR AlphaFoldDB; Q9LXV2; -.
DR SMR; Q9LXV2; -.
DR BioGRID; 16405; 3.
DR IntAct; Q9LXV2; 1.
DR STRING; 3702.AT5G12870.1; -.
DR PaxDb; Q9LXV2; -.
DR PRIDE; Q9LXV2; -.
DR EnsemblPlants; AT5G12870.1; AT5G12870.1; AT5G12870.
DR GeneID; 831127; -.
DR Gramene; AT5G12870.1; AT5G12870.1; AT5G12870.
DR KEGG; ath:AT5G12870; -.
DR Araport; AT5G12870; -.
DR TAIR; locus:2182275; AT5G12870.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_16_2_1; -.
DR InParanoid; Q9LXV2; -.
DR OMA; PCNDDFT; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q9LXV2; -.
DR PRO; PR:Q9LXV2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LXV2; baseline and differential.
DR Genevisible; Q9LXV2; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IMP:TAIR.
DR GO; GO:1901348; P:positive regulation of secondary cell wall biogenesis; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:2000652; P:regulation of secondary cell wall biogenesis; IMP:TAIR.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..280
FT /note="Transcription factor MYB46"
FT /id="PRO_0000323610"
FT DOMAIN 15..67
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 68..122
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 43..67
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 95..118
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 129..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 280 AA; 31541 MW; 0DE9FC2F4ED7FC92 CRC64;
MRKPEVAIAA STHQVKKMKK GLWSPEEDSK LMQYMLSNGQ GCWSDVAKNA GLQRCGKSCR
LRWINYLRPD LKRGAFSPQE EDLIIRFHSI LGNRWSQIAA RLPGRTDNEI KNFWNSTIKK
RLKKMSDTSN LINNSSSSPN TASDSSSNSA SSLDIKDIIG SFMSLQEQGF VNPSLTHIQT
NNPFPTGNMI SHPCNDDFTP YVDGIYGVNA GVQGELYFPP LECEEGDWYN ANINNHLDEL
NTNGSGNAPE GMRPVEEFWD LDQLMNTEVP SFYFNFKQSI
