MYB60_ARATH
ID MYB60_ARATH Reviewed; 280 AA.
AC Q8GYP5; D2CFP9; Q3E6Y6; Q9FRQ8; Q9SBG0;
DT 13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Transcription factor MYB60 {ECO:0000303|PubMed:9839469};
DE AltName: Full=Myb domain protein 60 {ECO:0000303|PubMed:9839469};
DE Short=AtMYB60 {ECO:0000303|PubMed:9839469};
GN Name=MYB60 {ECO:0000303|PubMed:9839469};
GN OrderedLocusNames=At1g08810 {ECO:0000312|Araport:AT1G08810};
GN ORFNames=F22O13.30 {ECO:0000312|EMBL:AAF99772.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION BY AUXIN; LIGHT; UV-LIGHT
RP AND COLD, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=9839469; DOI=10.1046/j.1365-313x.1998.00278.x;
RA Kranz H.D., Denekamp M., Greco R., Jin H.-L., Leyva A., Meissner R.C.,
RA Petroni K., Urzainqui A., Bevan M., Martin C., Smeekens S., Tonelli C.,
RA Paz-Ares J., Weisshaar B.;
RT "Towards functional characterisation of the members of the R2R3-MYB gene
RT family from Arabidopsis thaliana.";
RL Plant J. 16:263-276(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND BIOTECHNOLOGY.
RX PubMed=18317777; DOI=10.1007/s00299-008-0521-1;
RA Park J.-S., Kim J.-B., Cho K.-J., Cheon C.-I., Sung M.-K., Choung M.-G.,
RA Roh K.-H.;
RT "Arabidopsis R2R3-MYB transcription factor AtMYB60 functions as a
RT transcriptional repressor of anthocyanin biosynthesis in lettuce (Lactuca
RT sativa).";
RL Plant Cell Rep. 27:985-994(2008).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Qu L.-J., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, REPRESSION BY DROUGHT;
RP ABSCISIC ACID AND BLUE LIGHT, AND INDUCTION BY WHITE LIGHT.
RX PubMed=16005291; DOI=10.1016/j.cub.2005.05.048;
RA Cominelli E., Galbiati M., Vavasseur A., Conti L., Sala T., Vuylsteke M.,
RA Leonhardt N., Dellaporta S.L., Tonelli C.;
RT "A guard-cell-specific MYB transcription factor regulates stomatal
RT movements and plant drought tolerance.";
RL Curr. Biol. 15:1196-1200(2005).
RN [10]
RP INDUCTION BY JASMONIC ACID AND SALICYLIC ACID, GENE FAMILY, AND
RP NOMENCLATURE.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [11]
RP TISSUE SPECIFICITY.
RX PubMed=18036199; DOI=10.1111/j.1365-313x.2007.03371.x;
RA Galbiati M., Simoni L., Pavesi G., Cominelli E., Francia P., Vavasseur A.,
RA Nelson T., Bevan M., Tonelli C.;
RT "Gene trap lines identify Arabidopsis genes expressed in stomatal guard
RT cells.";
RL Plant J. 53:750-762(2008).
RN [12]
RP TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=22088138; DOI=10.1186/1471-2229-11-162;
RA Cominelli E., Galbiati M., Albertini A., Fornara F., Conti L., Coupland G.,
RA Tonelli C.;
RT "DOF-binding sites additively contribute to guard cell-specificity of
RT AtMYB60 promoter.";
RL BMC Plant Biol. 11:162-162(2011).
RN [13]
RP FUNCTION, ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, AND INDUCTION BY
RP ABSCISIC ACID; SUCROSE AND AUXIN.
RC STRAIN=cv. Columbia;
RX PubMed=21637967; DOI=10.1007/s11103-011-9796-7;
RA Oh J.E., Kwon Y., Kim J.H., Noh H., Hong S.-W., Lee H.;
RT "A dual role for MYB60 in stomatal regulation and root growth of
RT Arabidopsis thaliana under drought stress.";
RL Plant Mol. Biol. 77:91-103(2011).
RN [14]
RP INDUCTION BY SCAP1.
RX PubMed=23453954; DOI=10.1016/j.cub.2013.02.001;
RA Negi J., Moriwaki K., Konishi M., Yokoyama R., Nakano T., Kusumi K.,
RA Hashimoto-Sugimoto M., Schroeder J.I., Nishitani K., Yanagisawa S., Iba K.;
RT "A Dof transcription factor, SCAP1, is essential for the development of
RT functional stomata in Arabidopsis.";
RL Curr. Biol. 23:479-484(2013).
RN [15]
RP TISSUE SPECIFICITY, REPRESSION BY ABSCISIC ACID, AND BIOTECHNOLOGY.
RC STRAIN=cv. Columbia;
RX PubMed=23828545; DOI=10.1093/jxb/ert180;
RA Rusconi F., Simeoni F., Francia P., Cominelli E., Conti L., Riboni M.,
RA Simoni L., Martin C.R., Tonelli C., Galbiati M.;
RT "The Arabidopsis thaliana MYB60 promoter provides a tool for the spatio-
RT temporal control of gene expression in stomatal guard cells.";
RL J. Exp. Bot. 64:3361-3371(2013).
CC -!- FUNCTION: Transcription factor involved in the regulation of gene (e.g.
CC drought-regulated and flavonoid biosynthetic genes) expression and
CC stomatal movements leading to negative regulation of responses to
CC drought and responses to other physiological stimuli (e.g. light)
CC (PubMed:16005291, PubMed:21637967). Promotes guard cell deflation in
CC response to water deficit. Triggers root growth upon osmotic stress
CC (e.g. mannitol containing medium) (PubMed:21637967).
CC {ECO:0000269|PubMed:16005291, ECO:0000269|PubMed:21637967}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Nucleus {ECO:0000255|PROSITE-
CC ProRule:PRU00625, ECO:0000269|PubMed:21637967}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Nucleus
CC {ECO:0000269|PubMed:21637967}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=MYB60.1 {ECO:0000303|PubMed:21637967};
CC IsoId=Q8GYP5-1; Sequence=Displayed;
CC Name=2; Synonyms=MYB60.2 {ECO:0000303|PubMed:21637967};
CC IsoId=Q8GYP5-2; Sequence=VSP_060033;
CC -!- TISSUE SPECIFICITY: Specifically expressed in guard cells
CC (PubMed:16005291, PubMed:18036199, PubMed:22088138, PubMed:23828545).
CC Present in seedlings, leaves, stems and flowers (PubMed:16005291,
CC PubMed:22088138). {ECO:0000269|PubMed:16005291,
CC ECO:0000269|PubMed:18036199, ECO:0000269|PubMed:22088138,
CC ECO:0000269|PubMed:23828545}.
CC -!- INDUCTION: Induced by jasmonic acid (JA) and salicylic acid (SA)
CC (PubMed:16463103). Triggered by auxin (IAA) in roots (PubMed:9839469,
CC PubMed:21637967). Stimulated by light, UV-light and cold
CC (PubMed:9839469). According to PubMed:16005291 and PubMed:23828545,
CC rapidly repressed by abscisic acid (ABA) in an ABI1-dependent manner.
CC But in contrast, according to PubMed:21637967, transiently induced by
CC ABA in seedlings (PubMed:16005291, PubMed:21637967, PubMed:23828545).
CC Rapidly repressed by drought. Activated by white light, but repressed
CC by blue light and darkness (PubMed:16005291). Transiently induced by
CC salt (NaCl) in seedlings. Induced by sucrose (PubMed:21637967).
CC Positively regulated by SCAP1 (PubMed:23453954).
CC {ECO:0000269|PubMed:16005291, ECO:0000269|PubMed:16463103,
CC ECO:0000269|PubMed:21637967, ECO:0000269|PubMed:23453954,
CC ECO:0000269|PubMed:23828545, ECO:0000269|PubMed:9839469}.
CC -!- DISRUPTION PHENOTYPE: Constitutive reduction of stomatal opening and
CC decreased wilting under water stress conditions resulting in abnormally
CC turgid and green leaves as well as an enhanced tolerance to drought,
CC and associated with an altered expression of some genes (e.g. ERD10,
CC ERD13, NHL3, SYP122, VPEgamma, ERF and ZAT genes) mainly involved in
CC response to stress. Normal stomatal closure in the dark and upon
CC abscisic acid (ABA) exposure. {ECO:0000269|PubMed:16005291}.
CC -!- BIOTECHNOLOGY: Mediates the inhibition of anthocyanin (e.g. cyanidin
CC and delphinidin) biosynthesis when expressed in lettuce L.sativa and
CC could thus be used for the development of new varieties of lettuce.
CC {ECO:0000269|PubMed:18317777}.
CC -!- BIOTECHNOLOGY: Promoter triggering guard cells-specific expression can
CC be used in tobacco (N.tabacum) and tomato (S.lycopersicum) to monitor
CC stomata genetic engineering. {ECO:0000269|PubMed:23828545}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF99772.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF062895; AAC83617.1; -; mRNA.
DR EMBL; DQ767951; ABG76203.1; -; mRNA.
DR EMBL; AC003981; AAF99772.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28350.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28351.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM61164.1; -; Genomic_DNA.
DR EMBL; AK117469; BAC42133.1; -; mRNA.
DR EMBL; AY519551; AAS10021.1; -; mRNA.
DR EMBL; BT005074; AAO50607.1; -; mRNA.
DR PIR; T00737; T00737.
DR PIR; T51667; T51667.
DR RefSeq; NP_001318958.1; NM_001331790.1. [Q8GYP5-2]
DR RefSeq; NP_172358.1; NM_100755.3. [Q8GYP5-1]
DR RefSeq; NP_973795.1; NM_202066.1. [Q8GYP5-2]
DR AlphaFoldDB; Q8GYP5; -.
DR SMR; Q8GYP5; -.
DR IntAct; Q8GYP5; 1.
DR STRING; 3702.AT1G08810.1; -.
DR PaxDb; Q8GYP5; -.
DR PRIDE; Q8GYP5; -.
DR ProteomicsDB; 178947; -.
DR ProteomicsDB; 191906; -. [Q8GYP5-1]
DR EnsemblPlants; AT1G08810.1; AT1G08810.1; AT1G08810. [Q8GYP5-1]
DR EnsemblPlants; AT1G08810.2; AT1G08810.2; AT1G08810. [Q8GYP5-2]
DR EnsemblPlants; AT1G08810.3; AT1G08810.3; AT1G08810. [Q8GYP5-2]
DR GeneID; 837403; -.
DR Gramene; AT1G08810.1; AT1G08810.1; AT1G08810. [Q8GYP5-1]
DR Gramene; AT1G08810.2; AT1G08810.2; AT1G08810. [Q8GYP5-2]
DR Gramene; AT1G08810.3; AT1G08810.3; AT1G08810. [Q8GYP5-2]
DR KEGG; ath:AT1G08810; -.
DR Araport; AT1G08810; -.
DR TAIR; locus:2025565; AT1G08810.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_6_0_1; -.
DR InParanoid; Q8GYP5; -.
DR OMA; NGDHHED; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q8GYP5; -.
DR PRO; PR:Q8GYP5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GYP5; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0080148; P:negative regulation of response to water deprivation; IMP:UniProtKB.
DR GO; GO:0009737; P:response to abscisic acid; IMP:TAIR.
DR GO; GO:0009646; P:response to absence of light; IEP:UniProtKB.
DR GO; GO:0009733; P:response to auxin; IEP:UniProtKB.
DR GO; GO:0009637; P:response to blue light; IEP:UniProtKB.
DR GO; GO:0009409; P:response to cold; IEP:UniProtKB.
DR GO; GO:0009416; P:response to light stimulus; IEP:UniProtKB.
DR GO; GO:1902074; P:response to salt; IEP:UniProtKB.
DR GO; GO:0009744; P:response to sucrose; IEP:UniProtKB.
DR GO; GO:0009411; P:response to UV; IEP:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IEP:TAIR.
DR GO; GO:0010118; P:stomatal movement; IMP:TAIR.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome; Repeat;
KW S-nitrosylation; Transcription; Transcription regulation.
FT CHAIN 1..280
FT /note="Transcription factor MYB60"
FT /id="PRO_0000446251"
FT DOMAIN 9..65
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 66..116
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 37..61
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 89..112
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 118..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 204..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 128..149
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 208..247
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 49
FT /note="S-nitrosocysteine"
FT /evidence="ECO:0000250|UniProtKB:Q9SCU7"
FT MOD_RES 53
FT /note="S-nitrosocysteine"
FT /evidence="ECO:0000250|UniProtKB:Q9SCU7"
FT VAR_SEQ 1..88
FT /note="MGRPPCCDKIGIKKGPWTPEEDIILVSYIQEHGPGNWRSVPTNTGLLRCSKS
FT CRLRWTNYLRPGIKRGNFTPHEEGMIIHLQALLGNK -> MVVVR (in isoform
FT 2)"
FT /id="VSP_060033"
FT CONFLICT 2
FT /note="G -> P (in Ref. 2; ABG76203)"
FT /evidence="ECO:0000305"
FT CONFLICT 21
FT /note="E -> D (in Ref. 2; ABG76203)"
FT /evidence="ECO:0000305"
FT CONFLICT 158
FT /note="L -> V (in Ref. 1; AAC83617)"
FT /evidence="ECO:0000305"
FT CONFLICT 186
FT /note="F -> L (in Ref. 2; ABG76203)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="W -> R (in Ref. 1; AAC83617)"
FT /evidence="ECO:0000305"
FT CONFLICT 267
FT /note="M -> V (in Ref. 2; ABG76203)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 280 AA; 32017 MW; FE01ABD2241F16FA CRC64;
MGRPPCCDKI GIKKGPWTPE EDIILVSYIQ EHGPGNWRSV PTNTGLLRCS KSCRLRWTNY
LRPGIKRGNF TPHEEGMIIH LQALLGNKWA SIASYLPQRT DNDIKNYWNT HLKKKLNKSD
SDERSRSENI ALQTSSTRNT INHRSTYASS TENISRLLEG WMRASPKSST STTFLEHKMQ
NRTNNFIDHH SDQFPYEQLQ GSWEEGHSKG INGDDDQGIK NSENNNGDDV HHEDGDHEDD
DDHNATPPLT FIEKWLLEET STTGGQMEEM SHLMELSNML
