MYB61_ARATH
ID MYB61_ARATH Reviewed; 366 AA.
AC Q8VZQ2; Q9SAV9; Q9SBF9;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Transcription factor MYB61 {ECO:0000305};
DE AltName: Full=Myb-related protein 61 {ECO:0000305};
DE Short=AtMYB61 {ECO:0000303|PubMed:16005292};
GN Name=MYB61 {ECO:0000303|PubMed:9839469};
GN OrderedLocusNames=At1g09540 {ECO:0000312|Araport:AT1G09540};
GN ORFNames=F14J9.20 {ECO:0000312|EMBL:AAC33214.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 76-366.
RC STRAIN=cv. Columbia;
RX PubMed=9839469; DOI=10.1046/j.1365-313x.1998.00278.x;
RA Kranz H.D., Denekamp M., Greco R., Jin H.-L., Leyva A., Meissner R.C.,
RA Petroni K., Urzainqui A., Bevan M., Martin C., Smeekens S., Tonelli C.,
RA Paz-Ares J., Weisshaar B.;
RT "Towards functional characterisation of the members of the R2R3-MYB gene
RT family from Arabidopsis thaliana.";
RL Plant J. 16:263-276(1998).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=16005292; DOI=10.1016/j.cub.2005.06.041;
RA Liang Y.K., Dubos C., Dodd I.C., Holroyd G.H., Hetherington A.M.,
RA Campbell M.M.;
RT "AtMYB61, an R2R3-MYB transcription factor controlling stomatal aperture in
RT Arabidopsis thaliana.";
RL Curr. Biol. 15:1201-1206(2005).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19401413; DOI=10.1093/jxb/erp102;
RA Arsovski A.A., Villota M.M., Rowland O., Subramaniam R., Western T.L.;
RT "MUM ENHANCERS are important for seed coat mucilage production and mucilage
RT secretory cell differentiation in Arabidopsis thaliana.";
RL J. Exp. Bot. 60:2601-2612(2009).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=22708996; DOI=10.1111/j.1469-8137.2012.04201.x;
RA Romano J.M., Dubos C., Prouse M.B., Wilkins O., Hong H., Poole M.,
RA Kang K.Y., Li E., Douglas C.J., Western T.L., Mansfield S.D.,
RA Campbell M.M.;
RT "AtMYB61, an R2R3-MYB transcription factor, functions as a pleiotropic
RT regulator via a small gene network.";
RL New Phytol. 195:774-786(2012).
RN [9]
RP FUNCTION.
RX PubMed=23741471; DOI=10.1371/journal.pone.0065132;
RA Prouse M.B., Campbell M.M.;
RT "Interactions between the R2R3-MYB transcription factor, AtMYB61, and
RT target DNA binding sites.";
RL PLoS ONE 8:E65132-E65132(2013).
RN [10]
RP FUNCTION.
RX PubMed=28207974; DOI=10.1111/tpj.13509;
RA Matias-Hernandez L., Jiang W., Yang K., Tang K., Brodelius P.E., Pelaz S.;
RT "AaMYB1, and its orthologue AtMYB61, affect terpene metabolism and trichome
RT development in Artemisia annua and Arabidopsis thaliana.";
RL Plant J. 90:520-534(2017).
CC -!- FUNCTION: Transcription factor that coordinates a small network of
CC downstream target genes required for several aspects of plant growth
CC and development, such as xylem formation and xylem cell
CC differentiation, and lateral root formation (PubMed:22708996).
CC Regulates a specific set of target genes by binding DNA to the AC cis-
CC element 5'-ACCTAC-3' (PubMed:23741471). Functions as a transcriptional
CC regulator of stomatal closure. Plays a role the regulation of stomatal
CC pore size independently of abscisic acid (ABA) (PubMed:16005292).
CC Required for seed coat mucilage deposition during the development of
CC the seed coat epidermis (PubMed:19401413). Involved in the induction of
CC trichome initiation and branching by positively regulating GL1 and GL2.
CC Required for gibberellin (GA) biosynthesis and degradation by
CC positively affecting the expression of the enzymes that convert GA9
CC into the bioactive GA4, as well as the enzymes involved in the
CC degradation of GA4 (PubMed:28207974). {ECO:0000269|PubMed:16005292,
CC ECO:0000269|PubMed:19401413, ECO:0000269|PubMed:22708996,
CC ECO:0000269|PubMed:23741471, ECO:0000269|PubMed:28207974}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:16005292}.
CC -!- TISSUE SPECIFICITY: Expressed specifically in guard cells
CC (PubMed:16005292). Expressed in sink tissues, such as xylem, roots and
CC developing seeds (PubMed:22708996). {ECO:0000269|PubMed:16005292,
CC ECO:0000269|PubMed:22708996}.
CC -!- DISRUPTION PHENOTYPE: Increased stomatal pore opening
CC (PubMed:16005292). Reduced amount of seed mucilage (PubMed:19401413).
CC Early germination, slow growth, delayed flowering and senescence
CC (PubMed:22708996). {ECO:0000269|PubMed:16005292,
CC ECO:0000269|PubMed:19401413, ECO:0000269|PubMed:22708996}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC33214.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY519552; AAS10022.1; -; mRNA.
DR EMBL; AC003970; AAC33214.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28459.1; -; Genomic_DNA.
DR EMBL; AY063939; AAL36295.1; -; mRNA.
DR EMBL; AY096523; AAM20173.1; -; mRNA.
DR EMBL; AF062896; AAC83618.1; -; mRNA.
DR PIR; A86229; A86229.
DR PIR; T51668; T51668.
DR RefSeq; NP_172425.2; NM_100825.5.
DR AlphaFoldDB; Q8VZQ2; -.
DR SMR; Q8VZQ2; -.
DR STRING; 3702.AT1G09540.1; -.
DR iPTMnet; Q8VZQ2; -.
DR PaxDb; Q8VZQ2; -.
DR PRIDE; Q8VZQ2; -.
DR ProteomicsDB; 248915; -.
DR EnsemblPlants; AT1G09540.1; AT1G09540.1; AT1G09540.
DR GeneID; 837480; -.
DR Gramene; AT1G09540.1; AT1G09540.1; AT1G09540.
DR KEGG; ath:AT1G09540; -.
DR Araport; AT1G09540; -.
DR TAIR; locus:2012375; AT1G09540.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_10_1_1; -.
DR InParanoid; Q8VZQ2; -.
DR OMA; LAAPNWE; -.
DR OrthoDB; 1211917at2759; -.
DR PhylomeDB; Q8VZQ2; -.
DR PRO; PR:Q8VZQ2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8VZQ2; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0010087; P:phloem or xylem histogenesis; IMP:TAIR.
DR GO; GO:0010119; P:regulation of stomatal movement; IMP:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR GO; GO:0010214; P:seed coat development; IMP:TAIR.
DR GO; GO:0010089; P:xylem development; IMP:TAIR.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation.
FT CHAIN 1..366
FT /note="Transcription factor MYB61"
FT /id="PRO_0000439653"
FT DOMAIN 9..61
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 62..116
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 37..61
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 89..112
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 115..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 115..147
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 148..164
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 231
FT /note="P -> H (in Ref. 5; AAC83618)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 366 AA; 40891 MW; CFB4773458EB5C62 CRC64;
MGRHSCCYKQ KLRKGLWSPE EDEKLLTHIT NHGHGCWSSV PKLAGLQRCG KSCRLRWINY
LRPDLKRGAF SPEEENLIVE LHAVLGNRWS QIASRLPGRT DNEIKNLWNS SIKKKLKQRG
IDPNTHKPIS EVESFSDKDK PTTSNNKRSG NDHKSPSSSS ATNQDFFLER PSDLSDYFGF
QKLNFNSNLG LSVTTDSSLC SMIPPQFSPG NMVGSVLQTP VCVKPSISLP PDNNSSSPIS
GGDHVKLAAP NWEFQTNNNN TSNFFDNGGF SWSIPNSSTS SSQVKPNHNF EEIKWSEYLN
TPFFIGSTVQ SQTSQPIYIK SETDYLANVS NMTDPWSQNE NLGTTETSDV FSKDLQRMAV
SFGQSL
