MYB64_ARATH
ID MYB64_ARATH Reviewed; 423 AA.
AC Q9FY60; Q94CG3;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Transcription factor MYB64 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein 64 {ECO:0000303|PubMed:11597504};
DE Short=AtMYB64 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Protein UAS-TAGGED ROOT PATTERNING 10 {ECO:0000303|PubMed:23057675};
GN Name=MYB64 {ECO:0000303|PubMed:11597504};
GN Synonyms=URP10 {ECO:0000303|PubMed:23057675};
GN OrderedLocusNames=At5g11050 {ECO:0000312|Araport:AT5G11050};
GN ORFNames=T5K6.40 {ECO:0000312|EMBL:CAC03453.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L.-J., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP FUNCTION.
RX PubMed=23057675; DOI=10.1111/tpj.12049;
RA Waki T., Miyashima S., Nakanishi M., Ikeda Y., Hashimoto T., Nakajima K.;
RT "A GAL4-based targeted activation tagging system in Arabidopsis thaliana.";
RL Plant J. 73:357-367(2013).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=24068955; DOI=10.1371/journal.pgen.1003783;
RA Rabiger D.S., Drews G.N.;
RT "MYB64 and MYB119 are required for cellularization and differentiation
RT during female gametogenesis in Arabidopsis thaliana.";
RL PLoS Genet. 9:E1003783-E1003783(2013).
CC -!- FUNCTION: Transcription factor required for female gametophyte
CC fertility (PubMed:23057675, PubMed:24068955). Acts redundantly with
CC MYB119 to initiate the FG5 transition during female gametophyte
CC development. The FG5 transition represents the switch between free
CC nuclear divisions and cellularization-differentiation in female
CC gametophyte, and occurs during developmental stage FG5
CC (PubMed:24068955). {ECO:0000269|PubMed:23057675,
CC ECO:0000269|PubMed:24068955}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:24068955}.
CC -!- TISSUE SPECIFICITY: Expressed in ovary septum.
CC {ECO:0000269|PubMed:24068955}.
CC -!- DEVELOPMENTAL STAGE: During female gametogenesis, expressed in the
CC female gametophyte at stage FG4 (four-nucleate stage) in all four
CC nuclei. Expressed in the central cell in unfused polar nuclei at stage
CC FG5 and secondary nucleus at stage FG6. Expressed in the egg cell
CC nucleus during stages FG5 and FG6. Expressed very weakly in mature
CC female gametophytes (stage FG7). {ECO:0000269|PubMed:24068955}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but in the double mutant plants myb64 and myb119 the female
CC gametophytes fail to cellularize, resulting in enlarged coenocytes with
CC supernumerary nuclei. {ECO:0000269|PubMed:24068955}.
CC -!- MISCELLANEOUS: The gain-of-function mutant upd10-D (T-DNA tagging) is
CC infertile. {ECO:0000269|PubMed:23057675}.
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DR EMBL; AY032854; AAK52088.2; -; mRNA.
DR EMBL; AY519620; AAS10090.1; -; mRNA.
DR EMBL; AL391222; CAC03453.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91629.1; -; Genomic_DNA.
DR PIR; T51794; T51794.
DR RefSeq; NP_196666.1; NM_121143.2.
DR AlphaFoldDB; Q9FY60; -.
DR SMR; Q9FY60; -.
DR STRING; 3702.AT5G11050.1; -.
DR PaxDb; Q9FY60; -.
DR PRIDE; Q9FY60; -.
DR EnsemblPlants; AT5G11050.1; AT5G11050.1; AT5G11050.
DR GeneID; 830972; -.
DR Gramene; AT5G11050.1; AT5G11050.1; AT5G11050.
DR KEGG; ath:AT5G11050; -.
DR Araport; AT5G11050; -.
DR TAIR; locus:2184906; AT5G11050.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_034700_1_0_1; -.
DR InParanoid; Q9FY60; -.
DR OMA; KENMMFG; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q9FY60; -.
DR PRO; PR:Q9FY60; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FY60; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0051302; P:regulation of cell division; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR017884; SANT_dom.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..423
FT /note="Transcription factor MYB64"
FT /id="PRO_0000440859"
FT DOMAIN 100..155
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 156..206
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 128..151
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 179..202
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 193..230
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 215..230
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 287
FT /note="E -> G (in Ref. 2; AAK52088)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 423 AA; 48086 MW; 05AA2024C4FE6A8F CRC64;
MEEQKIQEKS LAHGAAPPLT AVERFLNGQK NEALCFKKQE RSIDRPIVKT TRAIEIRNEN
KENMMFGPRK EKNLAVIGEI VVKGAAKDYT CKDITKKQPY KNIIKGQWTA DEDRKLIKLV
MQHGERKWAV ISEKLEGRAG KQCRERWHNH LRPDIKKDSW SEEEERLLVE AHTRIGNKWA
EIAKLIQGRT ENSIKNHWNA TKRRQNSKRK HKRSKNADSN SDIDDLSPSA KRPRILEDYI
KNIENNDKNN GENIMTTSGN NVLSTSNYDQ FNSEDSTSSL LDDPYDEELV FLKNIFENHS
LENINLSQGT EITQSSSSGF MIENPKPKPN LYNNTFGTHL GAMVTEPANS SHLASDIYLS
DLLNGTASSS SSLTFLSSNN NEHAGENELL LPQANSTSER REMDLIEMLS GSTQGSNIWF
PLF
