MYB65_ARATH
ID MYB65_ARATH Reviewed; 553 AA.
AC Q9FR97; O65898; Q7DLH4;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Transcription factor MYB65 {ECO:0000303|PubMed:9839469};
DE AltName: Full=Myb-related protein 65 {ECO:0000303|PubMed:9839469};
DE Short=AtMYB65 {ECO:0000303|PubMed:9839469};
GN Name=MYB65 {ECO:0000303|PubMed:9839469};
GN OrderedLocusNames=At3g11440 {ECO:0000312|Araport:AT3G11440};
GN ORFNames=F24K9.11 {ECO:0000312|EMBL:AAG51434.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=11743113; DOI=10.1104/pp.010442;
RA Gocal G.F.W., Sheldon C.C., Gubler F., Moritz T., Bagnall D.J.,
RA MacMillan C.P., Li S.F., Parish R.W., Dennis E.S., Weigel D., King R.W.;
RT "GAMYB-like genes, flowering, and gibberellin signaling in Arabidopsis.";
RL Plant Physiol. 127:1682-1693(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: A genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE OF 84-300, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=9839469; DOI=10.1046/j.1365-313x.1998.00278.x;
RA Kranz H.D., Denekamp M., Greco R., Jin H.-L., Leyva A., Meissner R.C.,
RA Petroni K., Urzainqui A., Bevan M., Martin C., Smeekens S., Tonelli C.,
RA Paz-Ares J., Weisshaar B.;
RT "Towards functional characterisation of the members of the R2R3-MYB gene
RT family from Arabidopsis thaliana.";
RL Plant J. 16:263-276(1998).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 84-128.
RC STRAIN=cv. Landsberg erecta;
RA Romero I., Fuertes A., Benito M.J., Malpica J., Leyva A., Paz-Ares J.;
RT "One hundred R2R3-MYB genes in the genome of Arabidopsis thaliana.";
RL Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [9]
RP REPRESSION BY MICRORNA159.
RX PubMed=15226253; DOI=10.1242/dev.01206;
RA Achard P., Herr A., Baulcombe D.C., Harberd N.P.;
RT "Modulation of floral development by a gibberellin-regulated microRNA.";
RL Development 131:3357-3365(2004).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND REGULATION BY MIR159.
RC STRAIN=cv. Columbia;
RX PubMed=15722475; DOI=10.1105/tpc.104.027920;
RA Millar A.A., Gubler F.;
RT "The Arabidopsis GAMYB-like genes, MYB33 and MYB65, are microRNA-regulated
RT genes that redundantly facilitate anther development.";
RL Plant Cell 17:705-721(2005).
RN [11]
RP INDUCTION BY ETHYLENE AND SALICYLIC ACID.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [12]
RP REPRESSION BY MICRORNA159.
RX PubMed=17916625; DOI=10.1073/pnas.0707653104;
RA Allen R.S., Li J., Stahle M.I., Dubroue A., Gubler F., Millar A.A.;
RT "Genetic analysis reveals functional redundancy and the major target genes
RT of the Arabidopsis miR159 family.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:16371-16376(2007).
RN [13]
RP FUNCTION, DISRUPTION PHENOTYPE, AND REPRESSION BY MICRORNA159.
RC STRAIN=cv. Columbia;
RX PubMed=20699403; DOI=10.1104/pp.110.160630;
RA Alonso-Peral M.M., Li J., Li Y., Allen R.S., Schnippenkoetter W., Ohms S.,
RA White R.G., Millar A.A.;
RT "The microRNA159-regulated GAMYB-like genes inhibit growth and promote
RT programmed cell death in Arabidopsis.";
RL Plant Physiol. 154:757-771(2010).
RN [14]
RP TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=24278028; DOI=10.1371/journal.pgen.1003933;
RA Liang Y., Tan Z.-M., Zhu L., Niu Q.-K., Zhou J.-J., Li M., Chen L.-Q.,
RA Zhang X.-Q., Ye D.;
RT "MYB97, MYB101 and MYB120 function as male factors that control pollen
RT tube-synergid interaction in Arabidopsis thaliana fertilization.";
RL PLoS Genet. 9:E1003933-E1003933(2013).
CC -!- FUNCTION: Transcriptional activator of alpha-amylase expression that
CC binds to 5'-CAACTGTC-3' motif in target gene promoter
CC (PubMed:11743113). In vegetative tissues, inhibits growth by reducing
CC cell proliferation. Promotes the expression of aleurone-related genes
CC (e.g. CP1, CP, GASA1, BXL1 and BXL2) in seeds. Together with MYB33 and
CC MYB101, promotes the programmed cell death (PCD) the vacuolation of
CC protein storage vacuoles (PSVs) in the aleurone layers during seed
CC germination (PubMed:20699403). Together with MYB33, facilitates anther
CC and tapetum development (PubMed:15722475).
CC {ECO:0000269|PubMed:11743113, ECO:0000269|PubMed:15722475,
CC ECO:0000269|PubMed:20699403}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in roots (e.g. root tips), stems,
CC pollen, shoot apices, flowers and floral shoot tips, and, to a lower
CC extent, in leaves and siliques. {ECO:0000269|PubMed:11743113,
CC ECO:0000269|PubMed:15722475, ECO:0000269|PubMed:24278028}.
CC -!- DEVELOPMENTAL STAGE: In germinating seeds, present in the root tip and
CC in a linear array of up to 20 to 30 cells above the root tip. Strongly
CC expressed in the inflorescence apex, and, to some extent, in the
CC inflorescence stem, the vascular tissue, and the vascular tissue in
CC leaf primordia (PubMed:11743113). In flowers, expressed in sepals,
CC style, receptacle, anther filaments, and connective but not in anthers
CC themselves (PubMed:15722475). {ECO:0000269|PubMed:11743113,
CC ECO:0000269|PubMed:15722475}.
CC -!- INDUCTION: Repressed by microRNA159 (miR159a and miR159b) in vegetative
CC tissues (PubMed:20699403, PubMed:17916625, PubMed:15226253). Specific
CC expression in floral organs and in the shoot apices is regulated via
CC miR159-mediated degradation (PubMed:15722475). Slightly induced by
CC ethylene and salicylic acid (PubMed:16463103).
CC {ECO:0000269|PubMed:15226253, ECO:0000269|PubMed:15722475,
CC ECO:0000269|PubMed:16463103, ECO:0000269|PubMed:17916625,
CC ECO:0000269|PubMed:20699403}.
CC -!- DISRUPTION PHENOTYPE: Reduced expression levels of aleurone-related
CC genes (e.g. CP1, CP, GASA1, BXL1 and BXL2) in seeds. The triple mutant
CC myb33 myb65 myb101 has a male sterility and exhibits slower protein
CC storage vacuoles (PSVs) vacuolation rate in aleurone layers upon seed
CC germination (PubMed:20699403). The myb33 myb65 double mutant is
CC defective in anther development, with a tapetum undergoing hypertrophy
CC at the pollen mother cell stage, resulting in premeiotic abortion of
CC pollen development and male sterility. This sterility is conditional,
CC fertility being increased both under higher light or lower temperature
CC conditions (PubMed:15722475). {ECO:0000269|PubMed:15722475,
CC ECO:0000269|PubMed:20699403}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC83621.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF048840; AAG43496.1; -; Genomic_DNA.
DR EMBL; AY519585; AAS10055.1; -; mRNA.
DR EMBL; AC008153; AAG51434.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75047.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM65045.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM65047.1; -; Genomic_DNA.
DR EMBL; BT003940; AAO41985.1; -; mRNA.
DR EMBL; BT005120; AAO50653.1; -; mRNA.
DR EMBL; AF062899; AAC83621.1; ALT_FRAME; mRNA.
DR EMBL; Z95778; CAB09210.1; -; mRNA.
DR EMBL; Z95788; CAB09220.1; -; mRNA.
DR PIR; T51671; T51671.
DR PIR; T52125; T52125.
DR RefSeq; NP_001327042.1; NM_001337927.1.
DR RefSeq; NP_001327044.1; NM_001337926.1.
DR RefSeq; NP_187751.1; NM_111977.5.
DR AlphaFoldDB; Q9FR97; -.
DR SMR; Q9FR97; -.
DR IntAct; Q9FR97; 18.
DR STRING; 3702.AT3G11440.1; -.
DR PaxDb; Q9FR97; -.
DR PRIDE; Q9FR97; -.
DR EnsemblPlants; AT3G11440.1; AT3G11440.1; AT3G11440.
DR EnsemblPlants; AT3G11440.3; AT3G11440.3; AT3G11440.
DR EnsemblPlants; AT3G11440.4; AT3G11440.4; AT3G11440.
DR GeneID; 820317; -.
DR Gramene; AT3G11440.1; AT3G11440.1; AT3G11440.
DR Gramene; AT3G11440.3; AT3G11440.3; AT3G11440.
DR Gramene; AT3G11440.4; AT3G11440.4; AT3G11440.
DR KEGG; ath:AT3G11440; -.
DR Araport; AT3G11440; -.
DR TAIR; locus:2080697; AT3G11440.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_023548_1_1_1; -.
DR InParanoid; Q9FR97; -.
DR OMA; SIFSECI; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q9FR97; -.
DR PRO; PR:Q9FR97; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9FR97; baseline and differential.
DR GO; GO:0005634; C:nucleus; TAS:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0048653; P:anther development; IMP:UniProtKB.
DR GO; GO:0048655; P:anther wall tapetum morphogenesis; IMP:UniProtKB.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; TAS:TAIR.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:UniProtKB.
DR GO; GO:0045926; P:negative regulation of growth; IMP:TAIR.
DR GO; GO:0048235; P:pollen sperm cell differentiation; IGI:TAIR.
DR GO; GO:0043068; P:positive regulation of programmed cell death; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:1990019; P:protein storage vacuole organization; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR016310; GAMYB-like.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR PIRSF; PIRSF001693; Transcription_factor_GAMYB; 1.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; Differentiation; DNA-binding; Flowering;
KW Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..553
FT /note="Transcription factor MYB65"
FT /id="PRO_0000439243"
FT DOMAIN 38..90
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 91..145
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 66..90
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 118..141
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..34
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 553 AA; 61365 MW; D2ADC3D7EE1C39AF CRC64;
MSYTTATADS DDGMHSSIHN ESPAPDSISN GCRSRGKRSV LKKGPWTSTE DGILIDYVKK
HGEGNWNAVQ KHTSLARCGK SCRLRWANHL RPNLKKGAFS QEEEQLIVEM HAKMGNKWAQ
MAEHLPGRTD NEIKNYWNTR IKRRQRAGLP LYPPEIYVDD LHWSEEYTKS NIIRVDRRRR
HQDFLQLGNS KDNVLFDDLN FAASLLPAAS DLSDLVACNM LGTGASSSRY ESYMPPILPS
PKQIWESGSR FPMCSSNIKH EFQSPEHFQN TAVQKNPRSC SISPCDVDHH PYENQHSSHM
MMVPDSHTVT YGMHPTSKPL FGAVKLELPS FQYSETSAFD QWKTTPSPPH SDLLDSVDAY
IQSPPPSQVE ESDCFSSCDT GLLDMLLHEA KIKTSAKHSL LMSSPQKSFS STTCTTNVTQ
NVPRGSENLI KSGEYEDSQK YLGRSEITSP SQLSAGGFSS AFAGNVVKTE ELDQVWEPKR
VDITRPDVLL ASSWLDQGCY GIVSDTSSMS DALALLGGDD IGNSYVTVGS SSGQAPRGVG
SYGWTNMPPV WSL
