MYB75_ARATH
ID MYB75_ARATH Reviewed; 248 AA.
AC Q9FE25; Q38JL3; Q38JL4; Q38JL5; Q6R0B5; Q9ZTC7;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Transcription factor MYB75;
DE AltName: Full=Myb-related protein 75;
DE Short=AtMYB75;
DE AltName: Full=Production of anthocyanin pigment 1 protein;
DE AltName: Full=Suc-induced anthocyanin accumulation locus 1;
GN Name=MYB75; Synonyms=PAP1, SIAA1; OrderedLocusNames=At1g56650;
GN ORFNames=F25P12.92;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION BY SUCROSE;
RP UV LIGHT AND DROUGHT.
RC STRAIN=cv. Columbia;
RX PubMed=9839469; DOI=10.1046/j.1365-313x.1998.00278.x;
RA Kranz H.D., Denekamp M., Greco R., Jin H.-L., Leyva A., Meissner R.C.,
RA Petroni K., Urzainqui A., Bevan M., Martin C., Smeekens S., Tonelli C.,
RA Paz-Ares J., Weisshaar B.;
RT "Towards functional characterisation of the members of the R2R3-MYB gene
RT family from Arabidopsis thaliana.";
RL Plant J. 16:263-276(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=11148285; DOI=10.2307/3871236;
RA Borevitz J.O., Xia Y., Blount J., Dixon R.A., Lamb C.;
RT "Activation tagging identifies a conserved MYB regulator of phenylpropanoid
RT biosynthesis.";
RL Plant Cell 12:2383-2393(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND VARIANTS HIS-37; ASN-60;
RP THR-136; SER-147; 147-SER-GLY-148; ASN-160; ARG-167; THR-172; ASN-179;
RP MET-215 AND THR-220.
RC STRAIN=cv. C24, cv. Cvi-1, and cv. Landsberg erecta;
RX PubMed=16299184; DOI=10.1104/pp.105.066688;
RA Teng S., Keurentjes J., Bentsink L., Koornneef M., Smeekens S.;
RT "Sucrose-specific induction of anthocyanin biosynthesis in Arabidopsis
RT requires the MYB75/PAP1 gene.";
RL Plant Physiol. 139:1840-1852(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L.-J., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [9]
RP FUNCTION, AND INTERACTION WITH BHLH2.
RX PubMed=12917293; DOI=10.1242/dev.00681;
RA Zhang F., Gonzalez A., Zhao M., Payne C.T., Lloyd A.M.;
RT "A network of redundant bHLH proteins functions in all TTG1-dependent
RT pathways of Arabidopsis.";
RL Development 130:4859-4869(2003).
RN [10]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17147621; DOI=10.1111/j.1467-7652.2004.00094.x;
RA Matsui K., Tanaka H., Ohme-Takagi M.;
RT "Suppression of the biosynthesis of proanthocyanidin in Arabidopsis by a
RT chimeric PAP1 repressor.";
RL Plant Biotechnol. J. 2:487-493(2004).
RN [11]
RP FUNCTION, AND INTERACTION WITH BHLH12; BHLH1; BHLH2 AND BHLH42.
RX PubMed=15361138; DOI=10.1111/j.1365-313x.2004.02183.x;
RA Zimmermann I.M., Heim M.A., Weisshaar B., Uhrig J.F.;
RT "Comprehensive identification of Arabidopsis thaliana MYB transcription
RT factors interacting with R/B-like BHLH proteins.";
RL Plant J. 40:22-34(2004).
RN [12]
RP FUNCTION.
RX PubMed=15807784; DOI=10.1111/j.1365-313x.2005.02371.x;
RA Tohge T., Nishiyama Y., Hirai M.Y., Yano M., Nakajima J., Awazuhara M.,
RA Inoue E., Takahashi H., Goodenowe D.B., Kitayama M., Noji M., Yamazaki M.,
RA Saito K.;
RT "Functional genomics by integrated analysis of metabolome and transcriptome
RT of Arabidopsis plants over-expressing an MYB transcription factor.";
RL Plant J. 42:218-235(2005).
RN [13]
RP GENE FAMILY, AND INDUCTION BY JA AND NACL.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [14]
RP INDUCTION BY NITROGEN DEFICIENCY.
RX PubMed=17053893; DOI=10.1007/s00425-006-0414-x;
RA Lea U.S., Slimestad R., Smedvig P., Lillo C.;
RT "Nitrogen deficiency enhances expression of specific MYB and bHLH
RT transcription factors and accumulation of end products in the flavonoid
RT pathway.";
RL Planta 225:1245-1253(2007).
CC -!- FUNCTION: Transcription activator, when associated with BHLH12/MYC1,
CC EGL3, or GL3. Promotes the synthesis of. phenylpropanoid-derived
CC compounds such as anthocyanins and proanthocyanidin, probably together
CC with GL3 and BHLH2. Regulates the expression of CHS, DFRA, LDOX, and
CC BAN. {ECO:0000269|PubMed:11148285, ECO:0000269|PubMed:12917293,
CC ECO:0000269|PubMed:15361138, ECO:0000269|PubMed:15807784,
CC ECO:0000269|PubMed:16299184, ECO:0000269|PubMed:17147621}.
CC -!- SUBUNIT: Interacts with BHLH12/MYC1, BHLH1/GL3/MYC6, BHLH2/EGL3/MYC146,
CC and BHLH42/TT8. {ECO:0000269|PubMed:12917293,
CC ECO:0000269|PubMed:15361138}.
CC -!- INTERACTION:
CC Q9FE25; Q8W2F1: BHLH12; NbExp=3; IntAct=EBI-1545177, EBI-1546379;
CC Q9FE25; Q9CAD0: BHLH2; NbExp=5; IntAct=EBI-1545177, EBI-533398;
CC Q9FE25; Q9FN69: GL3; NbExp=2; IntAct=EBI-1545177, EBI-533348;
CC Q9FE25; Q9FT81: TT8; NbExp=5; IntAct=EBI-1545177, EBI-395790;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in mature plant aerial part.
CC Detected ubiquitously, with higher levels in rosette leaves and
CC flowers. {ECO:0000269|PubMed:17147621, ECO:0000269|PubMed:9839469}.
CC -!- INDUCTION: By jasmonic acid (JA), NaCl, sucrose, UV light, nitrogen
CC deficiency and drought. {ECO:0000269|PubMed:16463103,
CC ECO:0000269|PubMed:17053893, ECO:0000269|PubMed:9839469}.
CC -!- MISCELLANEOUS: This protein has reduced anthocyanins induction activity
CC in cv C24 and cv cvi-1 backgrounds.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC83630.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF062908; AAC83630.1; ALT_FRAME; mRNA.
DR EMBL; AF325123; AAG42001.1; -; Genomic_DNA.
DR EMBL; DQ222404; ABB03877.1; -; Genomic_DNA.
DR EMBL; DQ222405; ABB03878.1; -; Genomic_DNA.
DR EMBL; DQ222406; ABB03879.1; -; Genomic_DNA.
DR EMBL; AY519563; AAS10033.1; -; mRNA.
DR EMBL; AC009323; AAG09100.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33419.1; -; Genomic_DNA.
DR EMBL; AK221639; BAD95284.1; -; mRNA.
DR PIR; B96608; B96608.
DR PIR; T51680; T51680.
DR RefSeq; NP_176057.1; NM_104541.4.
DR AlphaFoldDB; Q9FE25; -.
DR SMR; Q9FE25; -.
DR BioGRID; 27345; 64.
DR IntAct; Q9FE25; 54.
DR STRING; 3702.AT1G56650.1; -.
DR PaxDb; Q9FE25; -.
DR PRIDE; Q9FE25; -.
DR ProteomicsDB; 251230; -.
DR EnsemblPlants; AT1G56650.1; AT1G56650.1; AT1G56650.
DR GeneID; 842120; -.
DR Gramene; AT1G56650.1; AT1G56650.1; AT1G56650.
DR KEGG; ath:AT1G56650; -.
DR Araport; AT1G56650; -.
DR TAIR; locus:2027523; AT1G56650.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_6_4_1; -.
DR InParanoid; Q9FE25; -.
DR OMA; HEPCCKI; -.
DR OrthoDB; 1072169at2759; -.
DR PhylomeDB; Q9FE25; -.
DR PRO; PR:Q9FE25; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FE25; baseline and differential.
DR Genevisible; Q9FE25; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IMP:TAIR.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0031542; P:positive regulation of anthocyanin biosynthetic process; IMP:TAIR.
DR GO; GO:0031540; P:regulation of anthocyanin biosynthetic process; IMP:TAIR.
DR GO; GO:0019430; P:removal of superoxide radicals; IMP:TAIR.
DR GO; GO:0009733; P:response to auxin; IEP:TAIR.
DR GO; GO:0009723; P:response to ethylene; IEP:TAIR.
DR GO; GO:0002239; P:response to oomycetes; IEP:TAIR.
DR GO; GO:0009745; P:sucrose mediated signaling; IMP:TAIR.
DR CDD; cd00167; SANT; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..248
FT /note="Transcription factor MYB75"
FT /id="PRO_0000285730"
FT DOMAIN 5..57
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 58..112
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 33..57
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 85..108
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT VARIANT 37
FT /note="P -> H (in strain: cv. cvi-1)"
FT /evidence="ECO:0000269|PubMed:16299184"
FT VARIANT 60
FT /note="S -> N (in strain: cv. Landsberg erecta)"
FT /evidence="ECO:0000269|PubMed:16299184"
FT VARIANT 136
FT /note="N -> T (in strain: cv. C24 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:16299184"
FT VARIANT 147..148
FT /note="TV -> SG (in strain: cv. Landsberg erecta)"
FT VARIANT 147
FT /note="T -> S (in strain: cv. C24)"
FT /evidence="ECO:0000269|PubMed:16299184"
FT VARIANT 160
FT /note="K -> N (in strain: cv. cvi-1)"
FT /evidence="ECO:0000269|PubMed:16299184"
FT VARIANT 167
FT /note="C -> R (in strain: cv. C24)"
FT /evidence="ECO:0000269|PubMed:16299184"
FT VARIANT 172
FT /note="I -> T (in strain: cv. C24 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:16299184"
FT VARIANT 179
FT /note="S -> N (in strain: cv. C24 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:16299184"
FT VARIANT 215
FT /note="I -> M (in strain: cv. C24 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:16299184"
FT VARIANT 220
FT /note="A -> T (in strain: cv. C24 and cv. Landsberg
FT erecta)"
FT /evidence="ECO:0000269|PubMed:16299184"
FT CONFLICT 246..247
FT /note="KF -> EL (in Ref. 4; AAS10033)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 248 AA; 28470 MW; B86103A9AB5CF442 CRC64;
MEGSSKGLRK GAWTTEEDSL LRQCINKYGE GKWHQVPVRA GLNRCRKSCR LRWLNYLKPS
IKRGKLSSDE VDLLLRLHRL LGNRWSLIAG RLPGRTANDV KNYWNTHLSK KHEPCCKIKM
KKRDITPIPT TPALKNNVYK PRPRSFTVNN DCNHLNAPPK VDVNPPCLGL NINNVCDNSI
IYNKDKKKDQ LVNNLIDGDN MWLEKFLEES QEVDILVPEA TTTEKGDTLA FDVDQLWSLF
DGETVKFD
