MYB77_ARATH
ID MYB77_ARATH Reviewed; 301 AA.
AC Q9SN12;
DT 20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=Transcription factor MYB77 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein 77 {ECO:0000303|PubMed:11597504};
DE Short=AtMYB77 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein R2 {ECO:0000303|PubMed:9678577};
DE Short=AtMYBR2 {ECO:0000303|PubMed:9678577};
GN Name=MYB77 {ECO:0000303|PubMed:11597504};
GN Synonyms=MYBR2 {ECO:0000303|PubMed:9678577};
GN OrderedLocusNames=At3g50060 {ECO:0000312|Araport:AT3G50060};
GN ORFNames=F3A4.140 {ECO:0000312|EMBL:CAB62114.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L.-J., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=9678577; DOI=10.1023/a:1006011002499;
RA Kirik V., Koelle K., Misera S., Baeumlein H.;
RT "Two novel MYB homologues with changed expression in late embryogenesis-
RT defective Arabidopsis mutants.";
RL Plant Mol. Biol. 37:819-827(1998).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [7]
RP INDUCTION.
RX PubMed=15173595; DOI=10.1073/pnas.0401707101;
RA Shin R., Schachtman D.P.;
RT "Hydrogen peroxide mediates plant root cell response to nutrient
RT deprivation.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:8827-8832(2004).
RN [8]
RP FUNCTION, INTERACTION WITH ARF1; ARF7 AND IAA19, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, INDUCTION BY AUXIN, AND DISRUPTION PHENOTYPE.
RX PubMed=17675404; DOI=10.1105/tpc.107.050963;
RA Shin R., Burch A.Y., Huppert K.A., Tiwari S.B., Murphy A.S.,
RA Guilfoyle T.J., Schachtman D.P.;
RT "The Arabidopsis transcription factor MYB77 modulates auxin signal
RT transduction.";
RL Plant Cell 19:2440-2453(2007).
RN [9]
RP FUNCTION, AND INTERACTION WITH PYL8.
RX PubMed=24894996; DOI=10.1126/scisignal.2005051;
RA Zhao Y., Xing L., Wang X., Hou Y.J., Gao J., Wang P., Duan C.G., Zhu X.,
RA Zhu J.K.;
RT "The ABA receptor PYL8 promotes lateral root growth by enhancing MYB77-
RT dependent transcription of auxin-responsive genes.";
RL Sci. Signal. 7:RA53-RA53(2014).
CC -!- FUNCTION: Transcription factor involved in auxin response. Functions in
CC auxin signal transduction and modulates lateral root growth. Interacts
CC with ARF response factors to promote auxin-responsive gene expression
CC (PubMed:17675404). In response to auxin, binds sequence-specific motifs
CC in the promoter of the auxin-responsive gene IAA19, and activates IAA19
CC transcription. The IAA19 transcription activation by MYB77 is enhanced
CC by direct interaction between MYB77 and PYL8 (PubMed:24894996).
CC {ECO:0000269|PubMed:17675404, ECO:0000269|PubMed:24894996}.
CC -!- SUBUNIT: Interacts with ARF1, ARF7 and IAA19 (PubMed:17675404).
CC Interacts with PYL8 (PubMed:24894996). {ECO:0000269|PubMed:17675404,
CC ECO:0000269|PubMed:24894996}.
CC -!- INTERACTION:
CC Q9SN12; Q8L7G0: ARF1; NbExp=2; IntAct=EBI-2324225, EBI-2324259;
CC Q9SN12; P93022: ARF7; NbExp=3; IntAct=EBI-2324225, EBI-632284;
CC Q9SN12; O24409: IAA19; NbExp=3; IntAct=EBI-2324225, EBI-632257;
CC Q9SN12; Q8H1R0: PYL10; NbExp=3; IntAct=EBI-2324225, EBI-2363213;
CC Q9SN12; Q9FJ50: PYL11; NbExp=3; IntAct=EBI-2324225, EBI-2363233;
CC Q9SN12; Q9FLB1: PYL5; NbExp=3; IntAct=EBI-2324225, EBI-2363181;
CC Q9SN12; Q8S8E3: PYL6; NbExp=3; IntAct=EBI-2324225, EBI-2363192;
CC Q9SN12; Q9FGM1: PYL8; NbExp=4; IntAct=EBI-2324225, EBI-2429535;
CC Q9SN12; Q84MC7: PYL9; NbExp=8; IntAct=EBI-2324225, EBI-2349513;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:17675404}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems and flowers. Expressed in
CC dry seeds (PubMed:9678577). Expressed in root vasculature, root tips
CC and lateral root (PubMed:17675404). {ECO:0000269|PubMed:17675404,
CC ECO:0000269|PubMed:9678577}.
CC -!- DEVELOPMENTAL STAGE: Expressed in leaves from the third leaf to rosette
CC leaves from six-week old plants. Expression follows a development-
CC dependent gradient in successive leaves. {ECO:0000269|PubMed:9678577}.
CC -!- INDUCTION: Induced by auxin (PubMed:17675404). Down-regulated by
CC potassium deprivation (PubMed:15173595). {ECO:0000269|PubMed:15173595,
CC ECO:0000269|PubMed:17675404}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|PubMed:17675404}.
CC -!- MISCELLANEOUS: Plants overexpressing MYB77 exhibit stunted roots and
CC shoots. {ECO:0000269|PubMed:17675404}.
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DR EMBL; AY519598; AAS10068.1; -; mRNA.
DR EMBL; AL132978; CAB62114.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78622.1; -; Genomic_DNA.
DR EMBL; AF424588; AAL11582.1; -; mRNA.
DR EMBL; AY124828; AAM70537.1; -; mRNA.
DR PIR; T45859; T45859.
DR RefSeq; NP_190575.1; NM_114866.4.
DR AlphaFoldDB; Q9SN12; -.
DR SMR; Q9SN12; -.
DR IntAct; Q9SN12; 20.
DR STRING; 3702.AT3G50060.1; -.
DR PaxDb; Q9SN12; -.
DR PRIDE; Q9SN12; -.
DR ProteomicsDB; 251029; -.
DR EnsemblPlants; AT3G50060.1; AT3G50060.1; AT3G50060.
DR GeneID; 824168; -.
DR Gramene; AT3G50060.1; AT3G50060.1; AT3G50060.
DR KEGG; ath:AT3G50060; -.
DR Araport; AT3G50060; -.
DR TAIR; locus:2083113; AT3G50060.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_14_0_1; -.
DR InParanoid; Q9SN12; -.
DR OMA; YMAEMQK; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q9SN12; -.
DR PRO; PR:Q9SN12; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SN12; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0048527; P:lateral root development; IMP:TAIR.
DR GO; GO:0010929; P:positive regulation of auxin mediated signaling pathway; IDA:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..301
FT /note="Transcription factor MYB77"
FT /id="PRO_0000442698"
FT DOMAIN 1..56
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 58..107
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 29..52
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 80..103
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 145..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 153..167
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 301 AA; 33111 MW; 9CCD5863E9D06DEC CRC64;
MADRVKGPWS QEEDEQLRRM VEKYGPRNWS AISKSIPGRS GKSCRLRWCN QLSPEVEHRP
FSPEEDETIV TARAQFGNKW ATIARLLNGR TDNAVKNHWN STLKRKCSGG VAVTTVTETE
EDQDRPKKRR SVSFDSAFAP VDTGLYMSPE SPNGIDVSDS STIPSPSSPV AQLFKPMPIS
GGFTVVPQPL PVEMSSSSED PPTSLSLSLP GAENTSSSHN NNNNALMFPR FESQMKINVE
ERGEGRRGEF MTVVQEMIKA EVRSYMAEMQ KTSGGFVVGG LYESGGNGGF RDCGVITPKV
E
