MYB80_ARATH
ID MYB80_ARATH Reviewed; 320 AA.
AC Q9XHV0;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Transcription factor MYB80 {ECO:0000305};
DE AltName: Full=MYB-related protein 103 {ECO:0000303|PubMed:10353220};
DE Short=AtMYB103 {ECO:0000303|PubMed:10353220};
DE AltName: Full=Myb-related protein 80 {ECO:0000305};
DE Short=AtMYB80 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Protein MALE STERILE 188 {ECO:0000303|PubMed:17727613};
GN Name=MYB80 {ECO:0000303|PubMed:11597504};
GN Synonyms=MS188 {ECO:0000303|PubMed:17727613},
GN MYB103 {ECO:0000303|PubMed:10353220};
GN OrderedLocusNames=At5g56110 {ECO:0000312|Araport:AT5G56110};
GN ORFNames=MDA7.17 {ECO:0000312|EMBL:BAB09293.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10353220; DOI=10.1093/oxfordjournals.pcp.a029548;
RA Li S.F., Higginson T., Parish R.W.;
RT "A novel MYB-related gene from Arabidopsis thaliana expressed in developing
RT anthers.";
RL Plant Cell Physiol. 40:343-347(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12848824; DOI=10.1046/j.1365-313x.2003.01791.x;
RA Higginson T., Li S.F., Parish R.W.;
RT "AtMYB103 regulates tapetum and trichome development in Arabidopsis
RT thaliana.";
RL Plant J. 35:177-192(2003).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP PRO-41.
RX PubMed=17727613; DOI=10.1111/j.1365-313x.2007.03254.x;
RA Zhang Z.B., Zhu J., Gao J.F., Wang C., Li H., Li H., Zhang H.Q., Zhang S.,
RA Wang D.M., Wang Q.X., Huang H., Xia H.J., Yang Z.N.;
RT "Transcription factor AtMYB103 is required for anther development by
RT regulating tapetum development, callose dissolution and exine formation in
RT Arabidopsis.";
RL Plant J. 52:528-538(2007).
RN [9]
RP DEVELOPMENTAL STAGE.
RX PubMed=21957980; DOI=10.1111/j.1744-7909.2011.01078.x;
RA Zhu J., Lou Y., Xu X., Yang Z.N.;
RT "A genetic pathway for tapetum development and function in Arabidopsis.";
RL J. Integr. Plant Biol. 53:892-900(2011).
RN [10]
RP FUNCTION.
RX PubMed=21673079; DOI=10.1105/tpc.110.082651;
RA Phan H.A., Iacuone S., Li S.F., Parish R.W.;
RT "The MYB80 transcription factor is required for pollen development and the
RT regulation of tapetal programmed cell death in Arabidopsis thaliana.";
RL Plant Cell 23:2209-2224(2011).
CC -!- FUNCTION: Transcription factor that binds to the DNA sequence 5'-
CC CCAACC-3'. Regulates directly PME5, UND and GLOX1 (PubMed:21673079).
CC Essential for tapetum development in anthers and microsporogenesis
CC (PubMed:12848824, PubMed:21673079). Regulates the timing of tapetal
CC programmed cell death (PCD) which is critical for pollen development.
CC May act through the activation of UND, encoding an A1 aspartic protease
CC (PubMed:21673079). Required for anther development by regulating
CC tapetum development, callose dissolution and exine formation. Acts
CC upstream of A6 and FAR2/MS2, two genes required for pollen exine
CC formation (PubMed:17727613). Negatively regulates trichome
CC endoreduplication and trichome branching (PubMed:12848824).
CC {ECO:0000269|PubMed:12848824, ECO:0000269|PubMed:17727613,
CC ECO:0000269|PubMed:21673079}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:17727613}.
CC -!- TISSUE SPECIFICITY: Expressed in the tapetum and middle layer of
CC developing anthers (PubMed:10353220). Expressed in trichomes
CC (PubMed:12848824). {ECO:0000269|PubMed:10353220,
CC ECO:0000269|PubMed:12848824}.
CC -!- DEVELOPMENTAL STAGE: During anther development, expressed from stage 4
CC to stage 6 in microsporocytes and tapetal cells. At the tetrad stage,
CC expressed predominantly in tapetal cells. During microgametogenesis,
CC expression decreases radically in the tapetum and microspores.
CC {ECO:0000269|PubMed:21957980}.
CC -!- DISRUPTION PHENOTYPE: Male sterility due to distorted pollen grains
CC lacking reticulate exine on their surface.
CC {ECO:0000269|PubMed:17727613}.
CC -!- MISCELLANEOUS: Plants silencing MYB80 have the majority of pollen
CC grains distorted in shape with reduced or no cytoplasmic content,
CC display early tapetal degeneration with large opaque bodies in the
CC tapetal cytoplasm, and trichomes on cauline and rosette leaves contain
CC increased nuclear DNA content and produces additional branches.
CC {ECO:0000269|PubMed:12848824}.
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DR EMBL; AF048839; AAD40692.1; -; Genomic_DNA.
DR EMBL; AY519639; AAS10109.1; -; mRNA.
DR EMBL; AB011476; BAB09293.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96722.1; -; Genomic_DNA.
DR EMBL; AB493793; BAH30631.1; -; mRNA.
DR RefSeq; NP_200422.1; NM_124993.1.
DR AlphaFoldDB; Q9XHV0; -.
DR SMR; Q9XHV0; -.
DR STRING; 3702.AT5G56110.1; -.
DR PaxDb; Q9XHV0; -.
DR PRIDE; Q9XHV0; -.
DR EnsemblPlants; AT5G56110.1; AT5G56110.1; AT5G56110.
DR GeneID; 835710; -.
DR Gramene; AT5G56110.1; AT5G56110.1; AT5G56110.
DR KEGG; ath:AT5G56110; -.
DR Araport; AT5G56110; -.
DR TAIR; locus:2161820; AT5G56110.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_17_2_1; -.
DR InParanoid; Q9XHV0; -.
DR OMA; GNDERNE; -.
DR OrthoDB; 1499244at2759; -.
DR PRO; PR:Q9XHV0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9XHV0; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003682; F:chromatin binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR GO; GO:0048658; P:anther wall tapetum development; IMP:TAIR.
DR GO; GO:0009555; P:pollen development; IGI:TAIR.
DR GO; GO:0010090; P:trichome morphogenesis; IMP:TAIR.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation.
FT CHAIN 1..320
FT /note="Transcription factor MYB80"
FT /id="PRO_0000436452"
FT DOMAIN 9..65
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 66..116
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 37..61
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 89..112
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 257..283
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 257..281
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 41
FT /note="P->L: In ms188-2; male sterility."
FT /evidence="ECO:0000269|PubMed:17727613"
SQ SEQUENCE 320 AA; 35983 MW; A6333EE5D22269CF CRC64;
MGRIPCCEKE NVKRGQWTPE EDNKLASYIA QHGTRNWRLI PKNAGLQRCG KSCRLRWTNY
LRPDLKHGQF SEAEEHIIVK FHSVLGNRWS LIAAQLPGRT DNDVKNYWNT KLKKKLSGMG
IDPVTHKPFS HLMAEITTTL NPPQVSHLAE AALGCFKDEM LHLLTKKRVD LNQINFSNHN
PNPNNFHEIA DNEAGKIKMD GLDHGNGIMK LWDMGNGFSY GSSSSSFGNE ERNDGSASPA
VAAWRGHGGI RTAVAETAAA EEEERRKLKG EVVDQEEIGS EGGRGDGMTM MRNHHHHQHV
FNVDNVLWDL QADDLINHMV
