MYB97_ARATH
ID MYB97_ARATH Reviewed; 389 AA.
AC Q9S773; A0MFA0;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Transcription factor MYB97 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein 97 {ECO:0000303|PubMed:11597504};
DE Short=AtMYB97 {ECO:0000303|PubMed:11597504};
GN Name=MYB97 {ECO:0000303|PubMed:11597504};
GN OrderedLocusNames=At4g26930 {ECO:0000312|Araport:AT4G26930};
GN ORFNames=F10M23.270 {ECO:0000312|EMBL:CAB36539.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=14500793; DOI=10.1104/pp.103.028241;
RA Becker J.D., Boavida L.C., Carneiro J., Haury M., Feijo J.A.;
RT "Transcriptional profiling of Arabidopsis tissues reveals the unique
RT characteristics of the pollen transcriptome.";
RL Plant Physiol. 133:713-725(2003).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=23791732; DOI=10.1016/j.cub.2013.05.021;
RA Leydon A.R., Beale K.M., Woroniecka K., Castner E., Chen J., Horgan C.,
RA Palanivelu R., Johnson M.A.;
RT "Three MYB transcription factors control pollen tube differentiation
RT required for sperm release.";
RL Curr. Biol. 23:1209-1214(2013).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=24278028; DOI=10.1371/journal.pgen.1003933;
RA Liang Y., Tan Z.-M., Zhu L., Niu Q.-K., Zhou J.-J., Li M., Chen L.-Q.,
RA Zhang X.-Q., Ye D.;
RT "MYB97, MYB101 and MYB120 function as male factors that control pollen
RT tube-synergid interaction in Arabidopsis thaliana fertilization.";
RL PLoS Genet. 9:E1003933-E1003933(2013).
CC -!- FUNCTION: Transcription activator (PubMed:24278028). Binds to 5'-
CC CAACTGTC-3' and/or 5'-TAACAAA-3' motif in target gene promoter to
CC promote their expression (By similarity). Together with MYB101 and
CC MYB120, functions as a male factor that controls pollen tube-synergid
CC interaction in fertilization. Required for pollen tube growth arrest
CC and sperm cell release in the female gametophyte, probably via the
CC regulation of pollen tube-specific gene expression (PubMed:24278028,
CC PubMed:23791732). {ECO:0000250|UniProtKB:O80883,
CC ECO:0000269|PubMed:23791732, ECO:0000269|PubMed:24278028}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:23791732, ECO:0000269|PubMed:24278028}.
CC Note=Detected in the vegetative nucleus of pollen tubes.
CC {ECO:0000269|PubMed:23791732}.
CC -!- TISSUE SPECIFICITY: Accumulates in pollen grains and pollen tube
CC (PubMed:14500793, PubMed:23791732, PubMed:24278028). Mostly expressed
CC in mature pollen grains, and, to a lower extent, in inflorescences and
CC siliques (PubMed:24278028). {ECO:0000269|PubMed:14500793,
CC ECO:0000269|PubMed:23791732, ECO:0000269|PubMed:24278028}.
CC -!- DEVELOPMENTAL STAGE: Accumulates in the pollen tube nucleus during
CC pollen tube growth through the pistil. {ECO:0000269|PubMed:23791732}.
CC -!- DISRUPTION PHENOTYPE: The triple mutant myb97 myb101 myb120 is impaired
CC in pollen tube growth arrest and subsequent sperm cell release in the
CC female gametophyte thus leading to a drastically reduced fertility.
CC Altered pollen tube-specific gene expression.
CC {ECO:0000269|PubMed:23791732, ECO:0000269|PubMed:24278028}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28651.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF176002; AAD53107.1; -; mRNA.
DR EMBL; AL035440; CAB36539.1; -; Genomic_DNA.
DR EMBL; AL161566; CAB79548.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85271.1; -; Genomic_DNA.
DR EMBL; DQ653226; ABK28651.1; ALT_SEQ; mRNA.
DR PIR; T04816; T04816.
DR RefSeq; NP_194423.1; NM_118827.2.
DR AlphaFoldDB; Q9S773; -.
DR SMR; Q9S773; -.
DR STRING; 3702.AT4G26930.1; -.
DR PaxDb; Q9S773; -.
DR PRIDE; Q9S773; -.
DR EnsemblPlants; AT4G26930.1; AT4G26930.1; AT4G26930.
DR GeneID; 828800; -.
DR Gramene; AT4G26930.1; AT4G26930.1; AT4G26930.
DR KEGG; ath:AT4G26930; -.
DR Araport; AT4G26930; -.
DR TAIR; locus:2116447; AT4G26930.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_023548_3_1_1; -.
DR InParanoid; Q9S773; -.
DR OMA; KKTWLAR; -.
DR OrthoDB; 1499244at2759; -.
DR PRO; PR:Q9S773; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9S773; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0048235; P:pollen sperm cell differentiation; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0080092; P:regulation of pollen tube growth; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..389
FT /note="Transcription factor MYB97"
FT /id="PRO_0000439244"
FT DOMAIN 16..68
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 69..123
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 44..68
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 96..119
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 131..159
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 389 AA; 43578 MW; C613335C619878CD CRC64;
MIVYGGGASE DGEGGGVVLK KGPWTVAEDE TLAAYVREYG EGNWNSVQKK TWLARCGKSC
RLRWANHLRP NLRKGSFTPE EERLIIQLHS QLGNKWARMA AQLPGRTDNE IKNYWNTRLK
RFQRQGLPLY PPEYSQNNHQ QQMYPQQPSS PLPSQTPASS FTFPLLQPPS LCPKRCYNTA
FSPKASYISS PTNFLVSSPT FLHTHSSLSS YQSTNPVYSM KHELSSNQIP YSASLGVYQV
SKFSDNGDCN QNLNTGLHTN TCQLLEDLME EAEALADSFR APKRRQIMAA LEDNNNNNNF
FSGGFGHRVS SNSLCSLQGL TPKEDESLQM NTMQDEDITK LLDWGSESEE ISNGQSSVIT
TENNLVLDDH QFAFLFPVDD DTNNLPGIC
