MYB98_ARATH
ID MYB98_ARATH Reviewed; 427 AA.
AC Q9S7L2; A0MF81; Q3HSE6;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Transcription factor MYB98 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein 98 {ECO:0000303|PubMed:11597504};
DE Short=AtMYB98 {ECO:0000303|PubMed:11597504};
GN Name=MYB98 {ECO:0000303|PubMed:11597504};
GN OrderedLocusNames=At4g18770 {ECO:0000312|Araport:AT4G18770};
GN ORFNames=F28A21.180 {ECO:0000312|EMBL:CAB37462.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=10743663; DOI=10.1046/j.1365-313x.2000.00666.x;
RA Kranz H., Scholz K., Weisshaar B.;
RT "c-MYB oncogene-like genes encoding three MYB repeats occur in all major
RT plant lineages.";
RL Plant J. 21:231-235(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=16214903; DOI=10.1105/tpc.105.034603;
RA Kasahara R.D., Portereiko M.F., Sandaklie-Nikolova L., Rabiger D.S.,
RA Drews G.N.;
RT "MYB98 is required for pollen tube guidance and synergid cell
RT differentiation in Arabidopsis.";
RL Plant Cell 17:2981-2992(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=17693534; DOI=10.1105/tpc.107.052076;
RA Punwani J.A., Rabiger D.S., Drews G.N.;
RT "MYB98 positively regulates a battery of synergid-expressed genes encoding
RT filiform apparatus localized proteins.";
RL Plant Cell 19:2557-2568(2007).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=17937500; DOI=10.1371/journal.pgen.0030171;
RA Jones-Rhoades M.W., Borevitz J.O., Preuss D.;
RT "Genome-wide expression profiling of the Arabidopsis female gametophyte
RT identifies families of small, secreted proteins.";
RL PLoS Genet. 3:1848-1861(2007).
RN [9]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=18410484; DOI=10.1111/j.1365-313x.2008.03514.x;
RA Punwani J.A., Rabiger D.S., Lloyd A., Drews G.N.;
RT "The MYB98 subcircuit of the synergid gene regulatory network includes
RT genes directly and indirectly regulated by MYB98.";
RL Plant J. 55:406-414(2008).
RN [10]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=23093426; DOI=10.1242/dev.081208;
RA Iwano M., Ngo Q.A., Entani T., Shiba H., Nagai T., Miyawaki A., Isogai A.,
RA Grossniklaus U., Takayama S.;
RT "Cytoplasmic Ca2+ changes dynamically during the interaction of the pollen
RT tube with synergid cells.";
RL Development 139:4202-4209(2012).
CC -!- FUNCTION: Transcription factor that binds to the motif 5'-GTAACNT-3' in
CC the promoter of target genes (e.g. DD11 and DD18) and promotes their
CC expression within synergid cells (e.g. in the filiform apparatus) in
CC ovules (PubMed:16214903, PubMed:17693534, PubMed:18410484,
CC PubMed:17937500). Required for the formation of the filiform apparatus
CC during synergid cell differentiation in the female gametophyte
CC (PubMed:16214903). Involved in pollen tube guidance to the micropyle
CC (PubMed:16214903, PubMed:17937500, PubMed:23093426).
CC {ECO:0000269|PubMed:16214903, ECO:0000269|PubMed:17693534,
CC ECO:0000269|PubMed:17937500, ECO:0000269|PubMed:18410484,
CC ECO:0000269|PubMed:23093426}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000255|PROSITE-ProRule:PRU00768, ECO:0000269|PubMed:17693534}.
CC Note=Localized to the nuclei of the synergid cells.
CC {ECO:0000269|PubMed:17693534}.
CC -!- TISSUE SPECIFICITY: Expressed at high levels in the synergid cells of
CC the female gametophyte, and at lower levels in the endosperm of young
CC seeds and the trichomes of young leaves and sepals.
CC {ECO:0000269|PubMed:16214903, ECO:0000269|PubMed:17693534}.
CC -!- DEVELOPMENTAL STAGE: Expressed in cellularized but not uncellularized
CC female gametophytes. {ECO:0000269|PubMed:16214903}.
CC -!- DISRUPTION PHENOTYPE: Impaired in pollen tube attraction associated
CC with a reduced cytoplasmic calcium burst during pollen tube tips
CC growing (PubMed:17937500, PubMed:23093426). Altered expression of
CC several ovule-specific genes (PubMed:17937500).
CC {ECO:0000269|PubMed:17937500, ECO:0000269|PubMed:23093426}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28639.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF176003; AAD53108.1; -; mRNA.
DR EMBL; DQ198082; ABA42061.1; -; mRNA.
DR EMBL; AL035526; CAB37462.1; -; Genomic_DNA.
DR EMBL; AL161549; CAB78879.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84088.1; -; Genomic_DNA.
DR EMBL; DQ446849; ABE66076.1; -; mRNA.
DR EMBL; DQ653207; ABK28639.1; ALT_SEQ; mRNA.
DR PIR; T04869; T04869.
DR RefSeq; NP_193612.1; NM_117993.3.
DR AlphaFoldDB; Q9S7L2; -.
DR SMR; Q9S7L2; -.
DR BioGRID; 12904; 4.
DR STRING; 3702.AT4G18770.1; -.
DR PaxDb; Q9S7L2; -.
DR PRIDE; Q9S7L2; -.
DR EnsemblPlants; AT4G18770.1; AT4G18770.1; AT4G18770.
DR GeneID; 827611; -.
DR Gramene; AT4G18770.1; AT4G18770.1; AT4G18770.
DR KEGG; ath:AT4G18770; -.
DR Araport; AT4G18770; -.
DR TAIR; locus:2124162; AT4G18770.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_9_0_1; -.
DR InParanoid; Q9S7L2; -.
DR OMA; CSYQENM; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q9S7L2; -.
DR PRO; PR:Q9S7L2; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9S7L2; baseline and differential.
DR Genevisible; Q9S7L2; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0009553; P:embryo sac development; IMP:TAIR.
DR GO; GO:0010183; P:pollen tube guidance; IMP:UniProtKB.
DR GO; GO:0045691; P:regulation of embryo sac central cell differentiation; IMP:UniProtKB.
DR GO; GO:0045697; P:regulation of synergid differentiation; IDA:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..427
FT /note="Transcription factor MYB98"
FT /id="PRO_0000234360"
FT DOMAIN 212..267
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 268..318
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 240..263
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 291..314
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT MOTIF 195..202
FT /note="Nuclear localization signal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOTIF 361..368
FT /note="Nuclear localization signal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT CONFLICT 82
FT /note="N -> T (in Ref. 2; ABA42061)"
FT /evidence="ECO:0000305"
FT CONFLICT 96..97
FT /note="DF -> EL (in Ref. 2; ABA42061)"
FT /evidence="ECO:0000305"
FT CONFLICT 113
FT /note="V -> M (in Ref. 2; ABA42061)"
FT /evidence="ECO:0000305"
FT CONFLICT 122
FT /note="H -> HP (in Ref. 2; ABA42061)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="Missing (in Ref. 2; ABA42061)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 427 AA; 50116 MW; 77E03B27C7A45538 CRC64;
MENFVDENGF ASLNQNIFTR DQEHMKEEDF PFEVVDQSKP TSFLQDFHHL DHDHQFDHHH
HHGSSSSHPL LSVQTTSSCI NNAPFEHCSY QENMVDFYET KPNLMNHHHF QAVENSYFTR
NHHHHQEINL VDEHDDPMDL EQNNMMMMRM IPFDYPPTET FKPMNFVMPD EISCVSADND
CYRATSFNKT KPFLTRKLSS SSSSSSWKET KKSTLVKGQW TAEEDRVLIQ LVEKYGLRKW
SHIAQVLPGR IGKQCRERWH NHLRPDIKKE TWSEEEDRVL IEFHKEIGNK WAEIAKRLPG
RTENSIKNHW NATKRRQFSK RKCRSKYPRP SLLQDYIKSL NMGALMASSV PARGRRRESN
NKKKDVVVAV EEKKKEEEVY GQDRIVPECV FTDDFGFNEK LLEEGCSIDS LLDDIPQPDI
DAFVHGL
