ID   A14_VAR67               Reviewed;          90 AA.
AC   P0DSQ3; P33839;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   23-FEB-2022, entry version 6.
DE   RecName: Full=Virion membrane protein A14;
GN   ORFNames=A14L, A15L;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Envelope protein which is a major component of the mature
CC       virion (MV) membrane. Essential for membrane biogenesis. Is required,
CC       together with A17, to form bona fide crescents, which can progress to
CC       form the immature virion (IV) membrane. A14 and A17 form a lattice that
CC       is stabilized by disulfide bonds and serves as an anchor within the
CC       viral membrane to which several other proteins important in virion
CC       structure and morphogenesis attach (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer; disulfide-linked. Interacts with A17.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}. Note=Component of the mature virion
CC       (MV) membrane. {ECO:0000250}.
CC   -!- PTM: Phosphorylated by viral F10 kinase, phosphorylation state is
CC       regulated by H1 phosphatase. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A14 family. {ECO:0000305}.
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DR   EMBL; X69198; CAA49059.1; -; Genomic_DNA.
DR   PIR; F36849; F36849.
DR   RefSeq; NP_042162.1; NC_001611.1.
DR   SMR; P0DSQ3; -.
DR   GeneID; 1486489; -.
DR   KEGG; vg:1486489; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008785; Poxvirus_A14.
DR   Pfam; PF05767; Pox_A14; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Membrane; Phosphoprotein; Reference proteome;
KW   Transmembrane; Transmembrane helix; Viral envelope protein; Virion.
FT   CHAIN           1..90
FT                   /note="Virion membrane protein A14"
FT                   /id="PRO_0000099243"
FT   TOPO_DOM        1..10
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        32..44
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        66..90
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         85
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250"
FT   DISULFID        71
FT                   /note="Interchain"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   90 AA;  9978 MW;  8F65DA9561678168 CRC64;
     MDMMLMIGNY FSGVLIAGII LLILSCIFAF IDFSKSTSPT RTWKVLSIMS FILGIIITVG
     MLIYSMWGKH CAPHRVSGVI HTNHSDISVN