MYBPP_MOUSE
ID MYBPP_MOUSE Reviewed; 932 AA.
AC Q5SUV2; Q80YS8; Q8R2Q9;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=MYCBP-associated protein;
DE AltName: Full=AMAM-1;
DE AltName: Full=AMY-1-binding protein 1;
DE Short=AMAP-1;
GN Name=Mycbpap {ECO:0000312|MGI:MGI:2388726}; Synonyms=Amap1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAH50808.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 195-932 (ISOFORM 1).
RC STRAIN=Czech II {ECO:0000312|EMBL:AAH27327.1};
RC TISSUE=Mammary tumor {ECO:0000312|EMBL:AAH27327.1}, and
RC Testis {ECO:0000312|EMBL:AAH50808.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=12151104; DOI=10.1016/s0167-4781(02)00411-6;
RA Yukitake H., Furusawa M., Taira T., Iguchi-Ariga S.M.M., Ariga H.;
RT "AMAP-1, a novel testis-specific AMY-1-binding protein, is differentially
RT expressed during the course of spermatogenesis.";
RL Biochim. Biophys. Acta 1577:126-132(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-559, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a role in spermatogenesis. May be involved in
CC synaptic processes (By similarity). {ECO:0000250|UniProtKB:Q69CM7,
CC ECO:0000250|UniProtKB:Q8TBZ2}.
CC -!- SUBUNIT: Interacts with MYCBP. {ECO:0000250|UniProtKB:Q8TBZ2}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q69CM7,
CC ECO:0000250|UniProtKB:Q8TBZ2}. Membrane {ECO:0000250|UniProtKB:Q69CM7,
CC ECO:0000250|UniProtKB:Q8TBZ2}. Note=Colocalizes with MYCBP in the
CC cytoplasm. {ECO:0000250|UniProtKB:Q69CM7,
CC ECO:0000250|UniProtKB:Q8TBZ2}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1 {ECO:0000269|PubMed:15489334};
CC IsoId=Q5SUV2-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|PubMed:15489334};
CC IsoId=Q5SUV2-2; Sequence=VSP_052544, VSP_052545;
CC -!- DEVELOPMENTAL STAGE: In testis, weakly expressed in 3-week old mice and
CC strongly expressed after expression of spermatids, especially after 10
CC weeks of age, during which time MYCBP/AMY-1 is also expressed.
CC {ECO:0000269|PubMed:12151104}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH27327.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL645809; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC027327; AAH27327.1; ALT_INIT; mRNA.
DR EMBL; BC050808; AAH50808.1; -; mRNA.
DR CCDS; CCDS48887.1; -. [Q5SUV2-1]
DR RefSeq; NP_733771.2; NM_170671.2. [Q5SUV2-1]
DR AlphaFoldDB; Q5SUV2; -.
DR STRING; 10090.ENSMUSP00000091477; -.
DR iPTMnet; Q5SUV2; -.
DR PhosphoSitePlus; Q5SUV2; -.
DR MaxQB; Q5SUV2; -.
DR PaxDb; Q5SUV2; -.
DR PRIDE; Q5SUV2; -.
DR ProteomicsDB; 252626; -. [Q5SUV2-1]
DR ProteomicsDB; 252627; -. [Q5SUV2-2]
DR Antibodypedia; 45319; 52 antibodies from 14 providers.
DR DNASU; 104601; -.
DR Ensembl; ENSMUST00000040692; ENSMUSP00000047579; ENSMUSG00000039110. [Q5SUV2-2]
DR Ensembl; ENSMUST00000093945; ENSMUSP00000091477; ENSMUSG00000039110. [Q5SUV2-1]
DR GeneID; 104601; -.
DR KEGG; mmu:104601; -.
DR UCSC; uc007kyw.2; mouse. [Q5SUV2-2]
DR UCSC; uc007kyx.2; mouse. [Q5SUV2-1]
DR CTD; 84073; -.
DR MGI; MGI:2388726; Mycbpap.
DR VEuPathDB; HostDB:ENSMUSG00000039110; -.
DR eggNOG; ENOG502QT8X; Eukaryota.
DR GeneTree; ENSGT00640000091565; -.
DR HOGENOM; CLU_014509_0_0_1; -.
DR InParanoid; Q5SUV2; -.
DR OrthoDB; 650849at2759; -.
DR PhylomeDB; Q5SUV2; -.
DR TreeFam; TF329788; -.
DR BioGRID-ORCS; 104601; 6 hits in 72 CRISPR screens.
DR PRO; PR:Q5SUV2; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5SUV2; protein.
DR Bgee; ENSMUSG00000039110; Expressed in spermatid and 150 other tissues.
DR ExpressionAtlas; Q5SUV2; baseline and differential.
DR Genevisible; Q5SUV2; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007268; P:chemical synaptic transmission; ISS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032707; MYCBPAP.
DR Pfam; PF14646; MYCBPAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Differentiation;
KW Membrane; Phosphoprotein; Reference proteome; Spermatogenesis.
FT CHAIN 1..932
FT /note="MYCBP-associated protein"
FT /id="PRO_0000302878"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 165..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 789..886
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..23
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 807..871
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 559
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 560
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q69CM7"
FT MOD_RES 566
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q69CM7"
FT VAR_SEQ 1..518
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_052544"
FT VAR_SEQ 519..534
FT /note="AISLTQDIFMDERKLL -> MTSLTTLHHKQLPGEC (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_052545"
FT CONFLICT 700
FT /note="I -> V (in Ref. 2; AAH27327)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 932 AA; 106452 MW; 6CDCF0A4D72E8A5C CRC64;
MMKKITERQS PLKLLEKKRA KAPEQPTPPI QEEPEPVSNV LQGDDILALA IKKEDLRKQH
VPQFEETGEK PVVTQKFIIR KLKPKDSSKR VYHLVAHPAT PDAATKPLDY SGPHDSFLSS
GQILPHQILG SLQDFKRIAV ARGNTQLAKL IHIQPCLMTL ISAKEEPKPK PPKEEERPSP
WAPPPQHNFL KNWRRHIALR KKQQEALSKH LKKPASELLM HTGESYRKIQ EEREVIDRAL
PTQHDGKATS WFWSPLEYLG DEMTGLLMTK KKTQRGLVEP ITHIRKPLSI QVETGLPAQK
DAWYRYTWDR SLFLIYRRKE LQSIMAELDF SQQDIDGLEV VGHGKPFSSV TVEEHLPPEK
IQKSSSEDTV FLDSLTNLSD MVPMPILGPS LLFCGKPACW VRGSNPEDKK NIGIGVRLTF
ETLEGERTSS ELTVVNNGTV AIWYNWRRRP HQDFFQDLKQ NKTQRFYFNN REGVILPGET
KHFTFFFKSL NAGIFRESWE FGTHPTLLGG AVLQVTLHAI SLTQDIFMDE RKLLETKLAA
HEAITIAQSV LQDLLRGIST PERTPSPVDA YLTEEDLFNY RNPRLHYQHQ VVQNLHQLWQ
QYRKAKATQK ETPSLRTPVP LLLVEKASGS ISPRNLVSEY SQLSPHQEMD TARKTRDFFL
SLKSSIGKKS VARKSIMEEL LVEEGPDRET TQRPWALKSI SPPKWNLCLE DFRQAVMTFP
EELQREDALI QLNKAAMELC QEQKPLQSDL LYQMCLQLWR DVIDSLVSQS LWLRNLLGLP
EKETVYLDLP DEQGQKSPPV TESKVTSGKA GKEDRRGGAQ EKKQLGTKDK DDKRGSKTPG
KEDRPNSKKL KPKDDKKVVK SASRDRLLSE DPPPDSTAPS QEPIDPLVME KYTQRLHAEV
YALLDNLVTD VMVLADELSS TKNVEESLRF CS
