MYC2_SOLLC
ID MYC2_SOLLC Reviewed; 689 AA.
AC A0A3Q7HRZ6; U5YT97;
DT 03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 10-APR-2019, sequence version 1.
DT 25-MAY-2022, entry version 17.
DE RecName: Full=Transcription factor MYC2 {ECO:0000303|PubMed:24483714};
DE Short=LeMYC2 {ECO:0000303|PubMed:24483714};
DE Short=SlMYC2 {ECO:0000303|PubMed:29528226};
DE AltName: Full=Basic helix-loop-helix protein 147 {ECO:0000303|PubMed:25612924};
DE AltName: Full=Transcription factor bHLH147 {ECO:0000303|PubMed:25612924};
DE AltName: Full=bHLH transcription factor bHLH147 {ECO:0000305};
GN Name=MYC2 {ECO:0000303|PubMed:24483714};
GN Synonyms=BHLH147 {ECO:0000303|PubMed:25612924},
GN JAMYC2 {ECO:0000303|PubMed:15231736};
GN OrderedLocusNames=Solyc08g076930 {ECO:0000305};
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX PubMed=24483714; DOI=10.1186/1471-2229-14-38;
RA Gupta N., Prasad V.B., Chattopadhyay S.;
RT "LeMYC2 acts as a negative regulator of blue light mediated
RT photomorphogenic growth, and promotes the growth of adult tomato plants.";
RL BMC Plant Biol. 14:38-38(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Heinz 1706;
RX PubMed=22660326; DOI=10.1038/nature11119;
RG Tomato Genome Consortium;
RT "The tomato genome sequence provides insights into fleshy fruit
RT evolution.";
RL Nature 485:635-641(2012).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=15231736; DOI=10.1101/gad.297704;
RA Boter M., Ruiz-Rivero O., Abdeen A., Prat S.;
RT "Conserved MYC transcription factors play a key role in jasmonate signaling
RT both in tomato and Arabidopsis.";
RL Genes Dev. 18:1577-1591(2004).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=25612924; DOI=10.1186/s12864-014-1209-2;
RA Sun H., Fan H.J., Ling H.Q.;
RT "Genome-wide identification and characterization of the bHLH gene family in
RT tomato.";
RL BMC Genomics 16:9-9(2015).
RN [5]
RP FUNCTION, INTERACTION WITH JAZ7, AND INDUCTION.
RX PubMed=28733419; DOI=10.1105/tpc.16.00953;
RA Du M., Zhao J., Tzeng D.T.W., Liu Y., Deng L., Yang T., Zhai Q., Wu F.,
RA Huang Z., Zhou M., Wang Q., Chen Q., Zhong S., Li C.B., Li C.;
RT "MYC2 orchestrates a hierarchical transcriptional cascade that regulates
RT jasmonate-mediated plant immunity in tomato.";
RL Plant Cell 29:1883-1906(2017).
RN [6]
RP FUNCTION, AND INDUCTION BY COLD STORAGE.
RX PubMed=29528226; DOI=10.1021/acs.jafc.8b00299;
RA Min D., Li F., Zhang X., Cui X., Shu P., Dong L., Ren C.;
RT "SlMYC2 involved in methyl jasmonate-induced tomato fruit chilling
RT tolerance.";
RL J. Agric. Food Chem. 66:3110-3117(2018).
RN [7]
RP FUNCTION, AND INDUCTION.
RX PubMed=30576511; DOI=10.1093/jxb/ery439;
RA Xu X., Fang P., Zhang H., Chi C., Song L., Xia X., Shi K., Zhou Y.,
RA Zhou J., Yu J.;
RT "Strigolactones positively regulate defense against root-knot nematodes in
RT tomato.";
RL J. Exp. Bot. 70:1325-1337(2019).
RN [8]
RP FUNCTION, AND INTERACTION WITH MED25; JAZ7 AND MTB1.
RX PubMed=30610166; DOI=10.1105/tpc.18.00405;
RA Liu Y., Du M., Deng L., Shen J., Fang M., Chen Q., Lu Y., Wang Q., Li C.,
RA Zhai Q.;
RT "MYC2 regulates the termination of jasmonate signaling via an
RT autoregulatory negative feedback loop.";
RL Plant Cell 31:106-127(2019).
CC -!- FUNCTION: Transcriptional activator that binds to the G-box motif (5'-
CC AACGTG-3') found in the promoter of the jasmonate-induced gene LAPA1
CC (PubMed:15231736). Acts as negative regulator of blue light-mediated
CC photomorphogenesis and positively regulates root growth
CC (PubMed:24483714). Promotes growth in response to the phytohormones
CC abscisic acid (ABA) and jasmonate (JA) (PubMed:24483714). Binds to the
CC G-box motif (5'-CACGTG-3') of the RBCS-3A gene promoter
CC (PubMed:24483714). Acts downstream of the jasmonate (JA) receptor to
CC orchestrate JA-mediated activation of plant responses
CC (PubMed:28733419). Positively regulates both wound-responsive and
CC pathogen-responsive genes through MYC2-targeted transcription factors
CC (MTFs) involved in early response to JA (PubMed:28733419). With JA2L
CC forms a transcription module that regulates wounding-responsive genes
CC (PubMed:28733419). With ERF.C3 forms a transcription module that
CC regulates pathogen-responsive genes (PubMed:28733419). Plays a critical
CC role in orchestrating JA-mediated defense gene expression during
CC Botrytis cinerea infection (PubMed:28733419). Regulates negatively
CC defense responses to root-knot nematodes, potentially by mediating
CC crosstalk among the hormones strigolactones, abscisic acid (ABA) and
CC jasmonate (JA) (PubMed:30576511). Regulates the termination of JA-
CC mediated defense responses by specifically binding the G-box (5'-
CC CACATG-3') motifs in the promoters of MTB1, MTB2 and MTB3, which are
CC transcription factors that negatively regulates JA signaling
CC (PubMed:30610166). May be involved in JA-induced chilling tolerance,
CC possibly by ameliorating the antioxidant enzyme system of fruit and
CC increasing proline and lycopene levels (PubMed:29528226).
CC {ECO:0000269|PubMed:15231736, ECO:0000269|PubMed:24483714,
CC ECO:0000269|PubMed:28733419, ECO:0000269|PubMed:29528226,
CC ECO:0000269|PubMed:30576511, ECO:0000269|PubMed:30610166}.
CC -!- SUBUNIT: Interacts (via N-terminus) with MED25 (PubMed:30610166).
CC Interacts (via N-terminus) with JAZ7 (PubMed:28733419,
CC PubMed:30610166). MED25 and JAZ7 compete with each other to bind to
CC MYC2 (PubMed:30610166). Interacts (via N-terminus) with MTB1
CC (PubMed:30610166). MTB1 and MED25 compete with each other to bind to
CC MYC2 (PubMed:30610166). {ECO:0000269|PubMed:28733419,
CC ECO:0000269|PubMed:30610166}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in roots, stems, leaves,
CC flowers and fruits. {ECO:0000269|PubMed:15231736}.
CC -!- INDUCTION: Induced by methyl jasmonate (PubMed:15231736,
CC PubMed:28733419). Induced by wounding (PubMed:15231736). Induced by
CC infection with the fungal pathogen Botrytis cinerea (PubMed:28733419).
CC Induced in fruit by storage in cold (PubMed:29528226). Induced by
CC hydrogen peroxide and infection with root-knot nematodes
CC (PubMed:30576511). {ECO:0000269|PubMed:15231736,
CC ECO:0000269|PubMed:28733419, ECO:0000269|PubMed:29528226,
CC ECO:0000269|PubMed:30576511}.
CC -!- MISCELLANEOUS: Plants over-expressing MYC2 exhibit increased number of
CC branches with reduced internode distance (PubMed:24483714). Plants
CC silencing MYC2 exhibit increased susceptibility to infection by the
CC fungal pathogen Botrytis cinerea (PubMed:28733419). Plants silencing
CC MYC2 show decreased susceptibility to infection by root-knot nematodes
CC (PubMed:30576511). {ECO:0000269|PubMed:24483714,
CC ECO:0000269|PubMed:28733419, ECO:0000269|PubMed:30576511}.
CC -!- CAUTION: Contains a degenerate basic motif not likely to bind DNA.
CC {ECO:0000255|PROSITE-ProRule:PRU00981}.
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DR EMBL; KF428776; AGZ94899.1; -; mRNA.
DR EMBL; CM001071; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001311412.1; NM_001324483.1.
DR AlphaFoldDB; A0A3Q7HRZ6; -.
DR SMR; A0A3Q7HRZ6; -.
DR STRING; 4081.Solyc08g076930.1.1; -.
DR EnsemblPlants; Solyc08g076930.1.1; Solyc08g076930.1.1.1; Solyc08g076930.1.
DR GeneID; 544165; -.
DR Gramene; Solyc08g076930.1.1; Solyc08g076930.1.1.1; Solyc08g076930.1.
DR KEGG; sly:544165; -.
DR OMA; AYGNENN; -.
DR Proteomes; UP000004994; Chromosome 8.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR045084; AIB/MYC-like.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR025610; MYC/MYB_N.
DR PANTHER; PTHR11514; PTHR11514; 1.
DR Pfam; PF14215; bHLH-MYC_N; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Growth regulation;
KW Jasmonic acid signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..689
FT /note="Transcription factor MYC2"
FT /id="PRO_0000447547"
FT DOMAIN 509..558
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 94..172
FT /note="JAZ-interaction domain"
FT /evidence="ECO:0000305|PubMed:30610166"
FT REGION 316..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 420..519
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 509..522
FT /note="Basic motif; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 523..558
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 563..602
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 442..460
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 469..519
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 563..585
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 45
FT /note="V -> A (in Ref. 1; AGZ94899)"
FT /evidence="ECO:0000305"
FT CONFLICT 365
FT /note="F -> L (in Ref. 1; AGZ94899)"
FT /evidence="ECO:0000305"
FT CONFLICT 671
FT /note="H -> N (in Ref. 1; AGZ94899)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 689 AA; 75041 MW; 9E2BE015FDFA1C93 CRC64;
MTEYSLPTMN LWNNSTSDDN VSMMEAFMSS DLSFWATNNS TSAAVVGVNS NLPHASSNTP
SVFAPSSSTS ASTLSAAATV DASKSMPFFN QETLQQRLQA LIDGARETWT YAIFWQSSVV
DFSSPSVLGW GDGYYKGEED KAKRKLSVSS PAYIAEQEHR KKVLRELNSL ISGAPPGTDD
AVDEEVTDTE WFFLISMTQS FVNGSGLPGQ ALYSSSPIWV AGTEKLAASH CERVRQAQGF
GLQTIVCIPS ANGVVELGST ELIVQSSDLM NKVRVLFNFS NDLGSGSWAV QPESDPSALW
LTDPSSSGME VRESLNTVQT NSVPSSNSNK QIAYGNENNH PSGNGQSCYN QQQQKNPPQQ
QTQGFFTREL NFSEFGFDGS SNRNGNSSVS CKPESGEILN FGDSTKKSAS SANVNLFTGQ
SQFGAGEENN NKNKKRSATS RGSNEEGMLS FVSGTVLPSS GMKSGGGGGE DSEHSDLEAS
VVKEADSSRV VEPEKRPRKR GRKPANGREE PLNHVEAERQ RREKLNQRFY ALRAVVPNVS
KMDKASLLGD AISYINELKS KLQNTESDKE DLKSQIEDLK KESRRPGPPP PPNQDLKMSS
HTGGKIVDVD IDVKIIGWDA MIRIQCNKKN HPAARLMAAL MELDLDVHHA SVSVVNDLMI
QQATVKMGSR HYTEEQLRVA LTSKIAETH
