MYCL_HUMAN
ID MYCL_HUMAN Reviewed; 364 AA.
AC P12524; A2A2C9; B4DJH2; Q14897; Q5QPL0; Q5QPL1; Q9NUE9;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 196.
DE RecName: Full=Protein L-Myc;
DE AltName: Full=Class E basic helix-loop-helix protein 38;
DE Short=bHLHe38;
DE AltName: Full=Protein L-Myc-1;
DE AltName: Full=V-myc myelocytomatosis viral oncogene homolog;
GN Name=MYCL; Synonyms=BHLHE38, LMYC, MYCL1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT SER-362.
RC TISSUE=Placenta;
RX PubMed=3322939; DOI=10.1101/gad.1.10.1311;
RA DePinho R.A., Hatton K.S., Tesfaye A., Yancopoulos G.D., Alt F.W.;
RT "The human myc gene family: structure and activity of L-myc and an L-myc
RT pseudogene.";
RL Genes Dev. 1:1311-1326(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, AND VARIANT
RP SER-362.
RC TISSUE=Placenta;
RX PubMed=2827002; DOI=10.1128/mcb.8.1.186-195.1988;
RA Kaye F., Battey J., Nau M., Brooks B., Seifter E., de Greve J., Birrer M.,
RA Sausville E., Minna J.;
RT "Structure and expression of the human L-myc gene reveal a complex pattern
RT of alternative mRNA processing.";
RL Mol. Cell. Biol. 8:186-195(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND VARIANT SER-362.
RC TISSUE=Thalamus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT SER-362.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PROTEIN SEQUENCE OF 165-179 (ISOFORM 1), AND SUBCELLULAR LOCATION.
RX PubMed=2548855; DOI=10.1002/j.1460-2075.1989.tb03573.x;
RA Ikegaki N., Minna J., Kennett R.H.;
RT "The human L-myc gene is expressed as two forms of protein in small cell
RT lung carcinoma cell lines: detection by monoclonal antibodies specific to
RT two myc homology box sequences.";
RL EMBO J. 8:1793-1799(1989).
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Binds DNA as a heterodimer with MAX.
CC -!- INTERACTION:
CC P12524-2; Q8NEC5: CATSPER1; NbExp=3; IntAct=EBI-18936665, EBI-744545;
CC P12524-2; O75909-2: CCNK; NbExp=3; IntAct=EBI-18936665, EBI-12010594;
CC P12524-2; Q7L8S5: OTUD6A; NbExp=3; IntAct=EBI-18936665, EBI-11960139;
CC P12524-2; P54646: PRKAA2; NbExp=3; IntAct=EBI-18936665, EBI-1383852;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:2548855}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=P12524-1; Sequence=Displayed;
CC Name=2; Synonyms=Short;
CC IsoId=P12524-2; Sequence=VSP_053766, VSP_015539, VSP_015540;
CC Name=3;
CC IsoId=P12524-3; Sequence=VSP_053766;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH11864.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA30248.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X07262; CAA30248.1; ALT_SEQ; Genomic_DNA.
DR EMBL; X07263; CAA30249.1; -; Genomic_DNA.
DR EMBL; M19720; AAA59879.1; -; Genomic_DNA.
DR EMBL; M19720; AAA59878.1; -; Genomic_DNA.
DR EMBL; AK296078; BAG58834.1; -; mRNA.
DR EMBL; AL033527; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471059; EAX07251.1; -; Genomic_DNA.
DR EMBL; BC011864; AAH11864.1; ALT_INIT; mRNA.
DR CCDS; CCDS30682.1; -. [P12524-1]
DR CCDS; CCDS44117.2; -. [P12524-2]
DR CCDS; CCDS53300.1; -. [P12524-3]
DR PIR; A27675; TVHUML.
DR RefSeq; NP_001028253.1; NM_001033081.2. [P12524-1]
DR RefSeq; NP_001028254.2; NM_001033082.2. [P12524-3]
DR RefSeq; NP_005367.2; NM_005376.4. [P12524-2]
DR AlphaFoldDB; P12524; -.
DR SMR; P12524; -.
DR BioGRID; 110695; 46.
DR IntAct; P12524; 44.
DR STRING; 9606.ENSP00000380494; -.
DR iPTMnet; P12524; -.
DR PhosphoSitePlus; P12524; -.
DR BioMuta; MYCL; -.
DR DMDM; 311033401; -.
DR MassIVE; P12524; -.
DR PaxDb; P12524; -.
DR PeptideAtlas; P12524; -.
DR PRIDE; P12524; -.
DR Antibodypedia; 17920; 144 antibodies from 27 providers.
DR DNASU; 4610; -.
DR Ensembl; ENST00000372815.1; ENSP00000361902.1; ENSG00000116990.11. [P12524-2]
DR Ensembl; ENST00000372816.3; ENSP00000361903.2; ENSG00000116990.11. [P12524-1]
DR Ensembl; ENST00000397332.2; ENSP00000380494.2; ENSG00000116990.11. [P12524-3]
DR GeneID; 4610; -.
DR KEGG; hsa:4610; -.
DR MANE-Select; ENST00000372816.3; ENSP00000361903.2; NM_001033081.3; NP_001028253.1.
DR UCSC; uc001cer.3; human. [P12524-1]
DR CTD; 4610; -.
DR DisGeNET; 4610; -.
DR GeneCards; MYCL; -.
DR HGNC; HGNC:7555; MYCL.
DR HPA; ENSG00000116990; Tissue enhanced (pancreas, skin).
DR MIM; 164850; gene.
DR neXtProt; NX_P12524; -.
DR OpenTargets; ENSG00000116990; -.
DR PharmGKB; PA31355; -.
DR VEuPathDB; HostDB:ENSG00000116990; -.
DR eggNOG; ENOG502QWSU; Eukaryota.
DR GeneTree; ENSGT00940000158613; -.
DR HOGENOM; CLU_052560_0_0_1; -.
DR InParanoid; P12524; -.
DR OMA; CHPRPVS; -.
DR OrthoDB; 877891at2759; -.
DR PhylomeDB; P12524; -.
DR TreeFam; TF106001; -.
DR PathwayCommons; P12524; -.
DR SignaLink; P12524; -.
DR SIGNOR; P12524; -.
DR BioGRID-ORCS; 4610; 13 hits in 1091 CRISPR screens.
DR ChiTaRS; MYCL; human.
DR GeneWiki; MYCL1; -.
DR GenomeRNAi; 4610; -.
DR Pharos; P12524; Tbio.
DR PRO; PR:P12524; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; P12524; protein.
DR Bgee; ENSG00000116990; Expressed in monocyte and 139 other tissues.
DR ExpressionAtlas; P12524; baseline and differential.
DR Genevisible; P12524; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005694; C:chromosome; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0003677; F:DNA binding; TAS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0045607; P:regulation of inner ear auditory receptor cell differentiation; IEA:Ensembl.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR002418; Tscrpt_reg_Myc.
DR InterPro; IPR012682; Tscrpt_reg_Myc_N.
DR Pfam; PF00010; HLH; 1.
DR Pfam; PF01056; Myc_N; 2.
DR PIRSF; PIRSF001705; Myc_protein; 1.
DR PRINTS; PR00044; LEUZIPPRMYC.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Direct protein sequencing; DNA-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..364
FT /note="Protein L-Myc"
FT /id="PRO_0000127333"
FT DOMAIN 281..333
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 41..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 111..172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 219..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 333..361
FT /note="Leucine-zipper"
FT COMPBIAS 41..63
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..257
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1
FT /note="M -> MCVCAGCRAAPSRRGAGPLQVAGGWSEGADM (in isoform 2
FT and isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_053766"
FT VAR_SEQ 166..206
FT /note="ENEEIDVVTVEKRQSLGIRKPVTITVRADPLDPCMKHFHIS -> GKDLPEP
FT SKRGPPHGWPKLCPCLRSGIGSSQALGPSPPLFG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_015539"
FT VAR_SEQ 207..364
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_015540"
FT VARIANT 362
FT /note="T -> S (in dbSNP:rs3134614)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:2827002, ECO:0000269|PubMed:3322939,
FT ECO:0000269|Ref.5"
FT /id="VAR_027802"
SQ SEQUENCE 364 AA; 40327 MW; 58F8A30A1C2ED6D4 CRC64;
MDYDSYQHYF YDYDCGEDFY RSTAPSEDIW KKFELVPSPP TSPPWGLGPG AGDPAPGIGP
PEPWPGGCTG DEAESRGHSK GWGRNYASII RRDCMWSGFS ARERLERAVS DRLAPGAPRG
NPPKASAAPD CTPSLEAGNP APAAPCPLGE PKTQACSGSE SPSDSENEEI DVVTVEKRQS
LGIRKPVTIT VRADPLDPCM KHFHISIHQQ QHNYAARFPP ESCSQEEASE RGPQEEVLER
DAAGEKEDEE DEEIVSPPPV ESEAAQSCHP KPVSSDTEDV TKRKNHNFLE RKRRNDLRSR
FLALRDQVPT LASCSKAPKV VILSKALEYL QALVGAEKRM ATEKRQLRCR QQQLQKRIAY
LTGY
