MYCP3_MYCTU
ID MYCP3_MYCTU Reviewed; 461 AA.
AC O53695; F2GM95; I6WY21; L0T376;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Mycosin-3 {ECO:0000303|PubMed:10974545};
DE EC=3.4.21.- {ECO:0000250|UniProtKB:O05461};
DE AltName: Full=MycP3 protease {ECO:0000305};
DE Flags: Precursor;
GN Name=mycP3 {ECO:0000303|PubMed:10974545};
GN OrderedLocusNames=Rv0291 {ECO:0000312|EMBL:CCP43021.1};
GN ORFNames=LH57_01590 {ECO:0000312|EMBL:AIR13014.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA Monaco A., King S., Sohrabi A.;
RT "Phylogenetic analysis of Mycobacterial species using whole genome
RT sequences.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=H37Rv;
RX PubMed=10974545; DOI=10.1016/s0378-1119(00)00277-8;
RA Brown G.D., Dave J.A., Gey van Pittius N.C., Stevens L., Ehlers M.R.,
RA Beyers A.D.;
RT "The mycosins of Mycobacterium tuberculosis H37Rv: a family of subtilisin-
RT like serine proteases.";
RL Gene 254:147-155(2000).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:10974545};
CC Single-pass membrane protein {ECO:0000255}. Note=Cell wall-associated.
CC {ECO:0000269|PubMed:10974545}.
CC -!- INDUCTION: Constitutively expressed during growth in culture.
CC {ECO:0000269|PubMed:10974545}.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; CP009480; AIR13014.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP43021.1; -; Genomic_DNA.
DR RefSeq; NP_214805.1; NC_000962.3.
DR RefSeq; WP_003401528.1; NZ_NVQJ01000026.1.
DR AlphaFoldDB; O53695; -.
DR SMR; O53695; -.
DR STRING; 83332.Rv0291; -.
DR MEROPS; S08.131; -.
DR PaxDb; O53695; -.
DR PRIDE; O53695; -.
DR DNASU; 886615; -.
DR GeneID; 45424265; -.
DR GeneID; 886615; -.
DR KEGG; mtu:Rv0291; -.
DR PATRIC; fig|83332.111.peg.327; -.
DR TubercuList; Rv0291; -.
DR eggNOG; COG1404; Bacteria.
DR HOGENOM; CLU_011263_13_1_11; -.
DR OMA; GPVQPME; -.
DR PhylomeDB; O53695; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005576; C:extracellular region; HDA:MTBBASE.
DR GO; GO:0005887; C:integral component of plasma membrane; HDA:MTBBASE.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR GO; GO:0005886; C:plasma membrane; IDA:MTBBASE.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0016485; P:protein processing; IBA:GO_Central.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR023834; T7SS_pept_S8A_mycosin.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR TIGRFAMs; TIGR03921; T7SS_mycosin; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Hydrolase; Membrane; Protease; Reference proteome;
KW Serine protease; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..461
FT /note="Mycosin-3"
FT /id="PRO_5007697062"
FT TRANSMEM 432..452
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 64..397
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 95
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 126
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 342
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 461 AA; 46127 MW; 7380B819B9356C2D CRC64;
MIRAAFACLA ATVVVAGWWT PPAWAIGPPV VDAAAQPPSG DPGPVAPMEQ RGACSVSGVI
PGTDPGVPTP SQTMLNLPAA WQFSRGEGQL VAIIDTGVQP GPRLPNVDAG GDFVESTDGL
TDCDGHGTLV AGIVAGQPGN DGFSGVAPAA RLLSIRAMST KFSPRTSGGD PQLAQATLDV
AVLAGAIVHA ADLGAKVINV STITCLPADR MVDQAALGAA IRYAAVDKDA VIVAAAGNTG
ASGSVSASCD SNPLTDLSRP DDPRNWAGVT SVSIPSWWQP YVLSVASLTS AGQPSKFSMP
GPWVGIAAPG ENIASVSNSG DGALANGLPD AHQKLVALSG TSYAAGYVSG VAALVRSRYP
GLNATEVVRR LTATAHRGAR ESSNIVGAGN LDAVAALTWQ LPAEPGGGAA PAKPVADPPV
PAPKDTTPRN VAFAGAAALS VLVGLTAATV AIARRRREPT E
