MYCP5_MYCTU
ID MYCP5_MYCTU Reviewed; 585 AA.
AC O53945; F2GIW7; I6YBM3; Q7D7Y3;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Mycosin-5 {ECO:0000303|PubMed:10974545};
DE EC=3.4.21.- {ECO:0000250|UniProtKB:O05461};
DE AltName: Full=MycP5 protease {ECO:0000305};
DE Flags: Precursor;
GN Name=mycP5 {ECO:0000303|PubMed:10974545};
GN OrderedLocusNames=Rv1796 {ECO:0000312|EMBL:CCP44562.1};
GN ORFNames=LH57_09820 {ECO:0000312|EMBL:AIR14545.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA Monaco A., King S., Sohrabi A.;
RT "Phylogenetic analysis of Mycobacterial species using whole genome
RT sequences.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP INDUCTION.
RC STRAIN=H37Rv;
RX PubMed=10974545; DOI=10.1016/s0378-1119(00)00277-8;
RA Brown G.D., Dave J.A., Gey van Pittius N.C., Stevens L., Ehlers M.R.,
RA Beyers A.D.;
RT "The mycosins of Mycobacterium tuberculosis H37Rv: a family of subtilisin-
RT like serine proteases.";
RL Gene 254:147-155(2000).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Constitutively expressed during growth in culture.
CC {ECO:0000269|PubMed:10974545}.
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR EMBL; CP009480; AIR14545.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP44562.1; -; Genomic_DNA.
DR RefSeq; NP_216312.1; NC_000962.3.
DR RefSeq; WP_003408854.1; NZ_NVQJ01000037.1.
DR PDB; 7NP7; EM; 4.03 A; P1/P2/P3=1-585.
DR PDB; 7NPR; EM; 3.82 A; P1/P2/P3=1-585.
DR PDB; 7NPS; EM; 3.81 A; P1/P2/P3=1-585.
DR PDBsum; 7NP7; -.
DR PDBsum; 7NPR; -.
DR PDBsum; 7NPS; -.
DR AlphaFoldDB; O53945; -.
DR SMR; O53945; -.
DR STRING; 83332.Rv1796; -.
DR TCDB; 3.A.24.4.1; the type vii or esx protein secretion system (t7ss) family.
DR PaxDb; O53945; -.
DR PRIDE; O53945; -.
DR GeneID; 885879; -.
DR KEGG; mtu:Rv1796; -.
DR PATRIC; fig|83332.111.peg.2001; -.
DR TubercuList; Rv1796; -.
DR eggNOG; COG1404; Bacteria.
DR HOGENOM; CLU_011263_13_1_11; -.
DR OMA; NSYCTEV; -.
DR PhylomeDB; O53945; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0016485; P:protein processing; IBA:GO_Central.
DR Gene3D; 3.40.50.200; -; 2.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR023834; T7SS_pept_S8A_mycosin.
DR Pfam; PF00082; Peptidase_S8; 2.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR TIGRFAMs; TIGR03921; T7SS_mycosin; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Hydrolase; Membrane; Protease;
KW Reference proteome; Serine protease; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..39
FT /evidence="ECO:0000255"
FT CHAIN 40..585
FT /note="Mycosin-5"
FT /id="PRO_5007696963"
FT TRANSMEM 552..572
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 83..521
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT REGION 163..269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 164..224
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 251..267
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 109
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 141
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 466
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 585 AA; 60028 MW; 854975AC9B5BC4F1 CRC64;
MQRFGTGSSR SWCGRAGTAT IAAVLLASGA LTGLPPAYAI SPPTIDPGAL PPDGPPGPLA
PMKQNAYCTE VGVLPGTDFQ LQPKYMEMLN LNEAWQFGRG DGVKVAVIDT GVTPHPRLPR
LIPGGDYVMA GGDGLSDCDA HGTLVASMIA AVPANGAVPL PSVPRRPVTI PTTETPPPPQ
TVTLSPVPPQ TVTVIPAPPP EEGVPPGAPV PGPEPPPAPG PQPPAVDRGG GTVTVPSYSG
GRKIAPIDNP RNPHPSAPSP ALGPPPDAFS GIAPGVEIIS IRQSSQAFGL KDPYTGDEDP
QTAQKIDNVE TMARAIVHAA NMGASVINIS DVMCMSARNV IDQRALGAAV HYAAVDKDAV
IVAAAGDGSK KDCKQNPIFD PLQPDDPRAW NAVTTVVTPS WFHDYVLTVG AVDANGQPLS
KMSIAGPWVS ISAPGTDVVG LSPRDDGLIN AIDGPDNSLL VPAGTSFSAA IVSGVAALVR
AKFPELSAYQ IINRLIHTAR PPARGVDNQV GYGVVDPVAA LTWDVPKGPA EPPKQLSAPL
VVPQPPAPRD MVPIWVAAGG LAGALLIGGA VFGTATLMRR SRKQQ
