MYH15_HUMAN
ID MYH15_HUMAN Reviewed; 1946 AA.
AC Q9Y2K3;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 5.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Myosin-15;
DE AltName: Full=Myosin heavy chain 15;
GN Name=MYH15; Synonyms=KIAA1000;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT TYR-504.
RC TISSUE=Brain;
RX PubMed=10231032; DOI=10.1093/dnares/6.1.63;
RA Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N.,
RA Tanaka A., Kotani H., Nomura N., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XIII. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 6:63-70(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [3]
RP IDENTIFICATION.
RX PubMed=11919279; DOI=10.1093/oxfordjournals.molbev.a004093;
RA Desjardins P.R., Burkman J.M., Shrager J.B., Allmond L.A., Stedman H.H.;
RT "Evolutionary implications of three novel members of the human sarcomeric
RT myosin heavy chain gene family.";
RL Mol. Biol. Evol. 19:375-393(2002).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
CC -!- FUNCTION: Muscle contraction. {ECO:0000250}.
CC -!- SUBUNIT: Muscle myosin is a hexameric protein that consists of 2 heavy
CC chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2
CC regulatory light chain subunits (MLC-2). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, myofibril. Note=Thick filaments of the
CC myofibrils.
CC -!- DOMAIN: The rodlike tail sequence is highly repetitive, showing cycles
CC of a 28-residue repeat pattern composed of 4 heptapeptides,
CC characteristic for alpha-helical coiled coils. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Myosin family. {ECO:0000305}.
CC -!- CAUTION: Represents a conventional myosin. This protein should not be
CC confused with the unconventional myosin-15 (MYO15). {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA76844.3; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB023217; BAA76844.3; ALT_INIT; mRNA.
DR EMBL; AC069499; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_055796.1; NM_014981.1.
DR RefSeq; XP_011510861.1; XM_011512559.2.
DR AlphaFoldDB; Q9Y2K3; -.
DR SMR; Q9Y2K3; -.
DR BioGRID; 116637; 12.
DR IntAct; Q9Y2K3; 5.
DR STRING; 9606.ENSP00000273353; -.
DR CarbonylDB; Q9Y2K3; -.
DR iPTMnet; Q9Y2K3; -.
DR PhosphoSitePlus; Q9Y2K3; -.
DR BioMuta; MYH15; -.
DR DMDM; 296439498; -.
DR jPOST; Q9Y2K3; -.
DR MassIVE; Q9Y2K3; -.
DR PaxDb; Q9Y2K3; -.
DR PeptideAtlas; Q9Y2K3; -.
DR PRIDE; Q9Y2K3; -.
DR ProteomicsDB; 85823; -.
DR Antibodypedia; 46526; 66 antibodies from 21 providers.
DR DNASU; 22989; -.
DR Ensembl; ENST00000273353.5; ENSP00000273353.4; ENSG00000144821.11.
DR GeneID; 22989; -.
DR KEGG; hsa:22989; -.
DR UCSC; uc003dxa.1; human.
DR CTD; 22989; -.
DR DisGeNET; 22989; -.
DR GeneCards; MYH15; -.
DR HGNC; HGNC:31073; MYH15.
DR HPA; ENSG00000144821; Tissue enhanced (brain, retina, tongue).
DR MIM; 609929; gene.
DR neXtProt; NX_Q9Y2K3; -.
DR PharmGKB; PA134958635; -.
DR VEuPathDB; HostDB:ENSG00000144821; -.
DR eggNOG; KOG0161; Eukaryota.
DR HOGENOM; CLU_000192_8_1_1; -.
DR InParanoid; Q9Y2K3; -.
DR OMA; RKQQCAI; -.
DR OrthoDB; 47111at2759; -.
DR PhylomeDB; Q9Y2K3; -.
DR TreeFam; TF314375; -.
DR PathwayCommons; Q9Y2K3; -.
DR SignaLink; Q9Y2K3; -.
DR BioGRID-ORCS; 22989; 10 hits in 1069 CRISPR screens.
DR ChiTaRS; MYH15; human.
DR GeneWiki; MYH15; -.
DR GenomeRNAi; 22989; -.
DR Pharos; Q9Y2K3; Tbio.
DR PRO; PR:Q9Y2K3; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q9Y2K3; protein.
DR Bgee; ENSG00000144821; Expressed in paraflocculus and 103 other tissues.
DR Genevisible; Q9Y2K3; HS.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0030016; C:myofibril; IEA:UniProtKB-SubCell.
DR GO; GO:0032982; C:myosin filament; IBA:GO_Central.
DR GO; GO:0016460; C:myosin II complex; IBA:GO_Central.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0000146; F:microfilament motor activity; IBA:GO_Central.
DR Gene3D; 1.20.5.370; -; 5.
DR Gene3D; 2.30.30.360; -; 1.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR001609; Myosin_head_motor_dom.
DR InterPro; IPR004009; Myosin_N.
DR InterPro; IPR008989; Myosin_S1_N.
DR InterPro; IPR002928; Myosin_tail.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014751; XRCC4-like_C.
DR Pfam; PF00063; Myosin_head; 1.
DR Pfam; PF02736; Myosin_N; 1.
DR Pfam; PF01576; Myosin_tail_1; 1.
DR PRINTS; PR00193; MYOSINHEAVY.
DR SMART; SM00242; MYSc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51456; MYOSIN_MOTOR; 1.
DR PROSITE; PS51844; SH3_LIKE; 1.
PE 1: Evidence at protein level;
KW Actin-binding; ATP-binding; Calmodulin-binding; Coiled coil; Cytoplasm;
KW Methylation; Motor protein; Muscle protein; Myosin; Nucleotide-binding;
KW Reference proteome; Thick filament.
FT CHAIN 1..1946
FT /note="Myosin-15"
FT /id="PRO_0000274233"
FT DOMAIN 49..99
FT /note="Myosin N-terminal SH3-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01190"
FT DOMAIN 103..790
FT /note="Myosin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00782"
FT DOMAIN 793..822
FT /note="IQ"
FT REGION 667..689
FT /note="Actin-binding"
FT /evidence="ECO:0000250"
FT REGION 769..783
FT /note="Actin-binding"
FT /evidence="ECO:0000250"
FT COILED 853..1946
FT /evidence="ECO:0000255"
FT BINDING 196..203
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT MOD_RES 147
FT /note="N6,N6,N6-trimethyllysine"
FT /evidence="ECO:0000255"
FT VARIANT 454
FT /note="R -> Q (in dbSNP:rs4299484)"
FT /id="VAR_030235"
FT VARIANT 504
FT /note="H -> Y (in dbSNP:rs9868484)"
FT /evidence="ECO:0000269|PubMed:10231032"
FT /id="VAR_030236"
FT VARIANT 949
FT /note="T -> I (in dbSNP:rs12638212)"
FT /id="VAR_030237"
FT VARIANT 1125
FT /note="T -> A (in dbSNP:rs3900940)"
FT /id="VAR_030238"
FT VARIANT 1467
FT /note="D -> N (in dbSNP:rs1078456)"
FT /id="VAR_046376"
SQ SEQUENCE 1946 AA; 224619 MW; 6CCE1836B64FC9C9 CRC64;
MVESCLLTFR AFFWWIALIK MDLSDLGEAA AFLRRSEAEL LLLQATALDG KKKCWIPDGE
NAYIEAEVKG SEDDGTVIVE TADGESLSIK EDKIQQMNPP EFEMIEDMAM LTHLNEASVL
HTLKRRYGQW MIYTYSGLFC VTINPYKWLP VYQKEVMAAY KGKRRSEAPP HIFAVANNAF
QDMLHNRENQ SILFTGESGA GKTVNSKHII QYFATIAAMI ESRKKQGALE DQIMQANTIL
EAFGNAKTLR NDNSSRFGKF IRMHFGARGM LSSVDIDIYL LEKSRVIFQQ AGERNYHIFY
QILSGQKELH DLLLVSANPS DFHFCSCGAV TVESLDDAEE LLATEQAMDI LGFLPDEKYG
CYKLTGAIMH FGNMKFKQKP REEQLEADGT ENADKAAFLM GINSSELVKC LIHPRIKVGN
EYVTRGQTIE QVTCAVGALS KSMYERMFKW LVARINRALD AKLSRQFFIG ILDITGFEIL
EYNSLEQLCI NFTNEKLQQF FNWHMFVLEQ EEYKKESIEW VSIGFGLDLQ ACIDLIEKPM
GILSILEEEC MFPKATDLTF KTKLFDNHFG KSVHLQKPKP DKKKFEAHFE LVHYAGVVPY
NISGWLEKNK DLLNETVVAV FQKSSNRLLA SLFENYMSTD SAIPFGEKKR KKGASFQTVA
SLHKENLNKL MTNLKSTAPH FVRCINPNVN KIPGILDPYL VLQQLRCNGV LEGTRICREG
FPNRLQYADF KQRYCILNPR TFPKSKFVSS RKAAEELLGS LEIDHTQYRF GITKVFFKAG
FLGQLEAIRD ERLSKVFTLF QARAQGKLMR IKFQKILEER DALILIQWNI RAFMAVKNWP
WMRLFFKIKP LVKSSEVGEE VAGLKEECAQ LQKALEKSEF QREELKAKQV SLTQEKNDLI
LQLQAEQETL ANVEEQCEWL IKSKIQLEAR VKELSERVEE EEEINSELTA RGRKLEDECF
ELKKEIDDLE TMLVKSEKEK RTTEHKVKNL TEEVEFLNED ISKLNRAAKV VQEAHQQTLD
DLHMEEEKLS SLSKANLKLE QQVDELEGAL EQERKARMNC ERELHKLEGN LKLNRESMEN
LESSQRHLAE ELRKKELELS QMNSKVENEK GLVAQLQKTV KELQTQIKDL KEKLEAERTT
RAKMERERAD LTQDLADLNE RLEEVGGSSL AQLEITKKQE TKFQKLHRDM EEATLHFETT
SASLKKRHAD SLAELEGQVE NLQQVKQKLE KDKSDLQLEV DDLLTRVEQM TRAKANAEKL
CTLYEERLHE ATAKLDKVTQ LANDLAAQKT KLWSESGEFL RRLEEKEALI NQLSREKSNF
TRQIEDLRGQ LEKETKSQSA LAHALQKAQR DCDLLREQYE EEQEVKAELH RTLSKVNAEM
VQWRMKYENN VIQRTEDLED AKKELAIRLQ EAAEAMGVAN ARNASLERAR HQLQLELGDA
LSDLGKVRSA AARLDQKQLQ SGKALADWKQ KHEESQALLD ASQKEVQALS TELLKLKNTY
EESIVGQETL RRENKNLQEE ISNLTNQVRE GTKNLTEMEK VKKLIEEEKT EVQVTLEETE
GALERNESKI LHFQLELLEA KAELERKLSE KDEEIENFRR KQQCTIDSLQ SSLDSEAKSR
IEVTRLKKKM EEDLNEMELQ LSCANRQVSE ATKSLGQLQI QIKDLQMQLD DSTQLNSDLK
EQVAVAERRN SLLQSELEDL RSLQEQTERG RRLSEEELLE ATERINLFYT QNTSLLSQKK
KLEADVARMQ KEAEEVVQEC QNAEEKAKKA AIEAANLSEE LKKKQDTIAH LERTRENMEQ
TITDLQKRLA EAEQMALMGS RKQIQKLESR VRELEGELEG EIRRSAEAQR GARRLERCIK
ELTYQAEEDK KNLSRMQTQM DKLQLKVQNY KQQVEVAETQ ANQYLSKYKK QQHELNEVKE
RAEVAESQVN KLKIKAREFG KKVQEE
