MYL10_MOUSE
ID MYL10_MOUSE Reviewed; 202 AA.
AC Q62082; Q62080;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Myosin regulatory light chain 10;
DE AltName: Full=Myosin light chain 2, lymphocyte-specific;
DE AltName: Full=Precursor lymphocyte-specific regulatory light chain;
GN Name=Myl10; Synonyms=Mylc2pl, Plrlc;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), ALTERNATIVE SPLICING, TISSUE
RP SPECIFICITY, AND INDUCTION.
RC TISSUE=Pre-B cell;
RX PubMed=1628631; DOI=10.1002/j.1460-2075.1992.tb05341.x;
RA Oltz E.M., Yancopoulos G.D., Morrow M.A., Rolink A., Lee G., Wong F.,
RA Kaplan K., Gillis S., Melchers F., Alt F.W.;
RT "A novel regulatory myosin light chain gene distinguishes pre-B cell
RT subsets and is IL-7 inducible.";
RL EMBO J. 11:2759-2767(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Comment=Additional isoforms seem to exist.;
CC Name=1;
CC IsoId=Q62082-1; Sequence=Displayed;
CC Name=2; Synonyms=PLRLC-C;
CC IsoId=Q62082-2; Sequence=VSP_031874;
CC Name=3;
CC IsoId=Q62082-3; Sequence=VSP_031873;
CC -!- TISSUE SPECIFICITY: Specifically expressed in precursor B- and T-
CC lymphocytes. {ECO:0000269|PubMed:1628631}.
CC -!- INDUCTION: Up-regulated by interleukin-7. {ECO:0000269|PubMed:1628631}.
CC -!- MISCELLANEOUS: This chain binds calcium.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA46794.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA46796.1; Type=Erroneous translation; Evidence={ECO:0000305};
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DR EMBL; X65979; CAA46794.1; ALT_INIT; mRNA.
DR EMBL; X65981; CAA46796.1; ALT_SEQ; mRNA.
DR EMBL; BC125528; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS80434.1; -. [Q62082-3]
DR PIR; S20990; S20990.
DR PIR; S20992; S20992.
DR RefSeq; NP_001078856.1; NM_001085387.2. [Q62082-3]
DR RefSeq; NP_001289950.1; NM_001303021.1. [Q62082-3]
DR AlphaFoldDB; Q62082; -.
DR SMR; Q62082; -.
DR BioGRID; 208548; 1.
DR STRING; 10090.ENSMUSP00000005611; -.
DR iPTMnet; Q62082; -.
DR PhosphoSitePlus; Q62082; -.
DR MaxQB; Q62082; -.
DR PaxDb; Q62082; -.
DR PeptideAtlas; Q62082; -.
DR PRIDE; Q62082; -.
DR ProteomicsDB; 286116; -. [Q62082-1]
DR ProteomicsDB; 286117; -. [Q62082-2]
DR ProteomicsDB; 286118; -. [Q62082-3]
DR Antibodypedia; 16757; 34 antibodies from 12 providers.
DR DNASU; 59310; -.
DR Ensembl; ENSMUST00000196436; ENSMUSP00000142495; ENSMUSG00000005474. [Q62082-3]
DR Ensembl; ENSMUST00000197186; ENSMUSP00000143165; ENSMUSG00000005474. [Q62082-3]
DR GeneID; 59310; -.
DR KEGG; mmu:59310; -.
DR UCSC; uc009aau.1; mouse. [Q62082-1]
DR UCSC; uc012eet.1; mouse. [Q62082-2]
DR CTD; 93408; -.
DR MGI; MGI:1891705; Myl10.
DR VEuPathDB; HostDB:ENSMUSG00000005474; -.
DR eggNOG; KOG0031; Eukaryota.
DR GeneTree; ENSGT00940000161811; -.
DR InParanoid; Q62082; -.
DR OrthoDB; 1421213at2759; -.
DR PhylomeDB; Q62082; -.
DR Reactome; R-MMU-445355; Smooth Muscle Contraction.
DR BioGRID-ORCS; 59310; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Myl10; mouse.
DR PRO; PR:Q62082; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q62082; protein.
DR Bgee; ENSMUSG00000005474; Expressed in seminiferous tubule of testis and 42 other tissues.
DR ExpressionAtlas; Q62082; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF00036; EF-hand_1; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calcium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..202
FT /note="Myosin regulatory light chain 10"
FT /id="PRO_0000322131"
FT DOMAIN 60..95
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 130..165
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 166..201
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 73
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 75
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 77
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 84
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT VAR_SEQ 1..71
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:1628631"
FT /id="VSP_031873"
FT VAR_SEQ 129..202
FT /note="TDPEETILHAFKVFDTEGKGFVKADFIKEKLMTQADRFSEEEVKQMFAAFPP
FT DVCGNLDYRNLCYVITHGEEKD -> SHCAELFYESSRPQSRGPLWGQGREWLAEARTQ
FT KRLYCTPSKCLTLKEKALSRLTSLRKSL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:1628631"
FT /id="VSP_031874"
SQ SEQUENCE 202 AA; 22511 MW; 97C976AD9E1A90BD CRC64;
MGQSSLDHGV QGPVAGTGDF GPLKEATATG FISCAQAPRR ARKRVEGTAS SNVFSMFDQS
QIQEFKEAFT IMDQNRDGFI DKEDLRDTFA ALGRINVKNE ELEAMVKEAP GPINFTVFLT
MFGEKLKGTD PEETILHAFK VFDTEGKGFV KADFIKEKLM TQADRFSEEE VKQMFAAFPP
DVCGNLDYRN LCYVITHGEE KD
