MYL1_MOUSE
ID MYL1_MOUSE Reviewed; 188 AA.
AC P05977; P05978;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Myosin light chain 1/3, skeletal muscle isoform;
DE Short=MLC1/MLC3;
DE Short=MLC1F/MLC3F;
DE AltName: Full=Myosin light chain alkali 1/2;
DE Short=Myosin light chain A1/A2;
GN Name=Myl1; Synonyms=Mylf;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING.
RX PubMed=6091905; DOI=10.1016/0092-8674(84)90198-3;
RA Robert B., Daubas P., Akimenko M.-A., Cohen A., Garner I., Guenet J.-L.,
RA Buckingham M.E.;
RT "A single locus in the mouse encodes both myosin light chains 1 and 3, a
RT second locus corresponds to a related pseudogene.";
RL Cell 39:129-140(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38.
RX PubMed=3194193; DOI=10.1093/nar/16.21.10037;
RA Cohen A., Barton P.J.R., Robert B., Garner I., Alonso S., Buckingham M.E.;
RT "Promoter analysis of myosin alkali light chain genes expressed in mouse
RT striated muscle.";
RL Nucleic Acids Res. 16:10037-10052(1988).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Non-regulatory myosin light chain required for proper
CC formation and/or maintenance of myofibers, and thus appropriate muscle
CC function. {ECO:0000250|UniProtKB:P05976}.
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains. Does
CC not bind calcium. {ECO:0000250|UniProtKB:P05976}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=MLC1;
CC IsoId=P05977-1; Sequence=Displayed;
CC Name=MLC3;
CC IsoId=P05977-2, P05978-1;
CC Sequence=VSP_038687;
CC -!- PTM: Isoform MLC3 is acetylated at position 2. {ECO:0000250}.
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DR EMBL; K02241; AAA39718.1; -; Genomic_DNA.
DR EMBL; K02237; AAA39718.1; JOINED; Genomic_DNA.
DR EMBL; K02238; AAA39718.1; JOINED; Genomic_DNA.
DR EMBL; K02239; AAA39718.1; JOINED; Genomic_DNA.
DR EMBL; K02240; AAA39718.1; JOINED; Genomic_DNA.
DR EMBL; K02241; AAA39719.1; -; Genomic_DNA.
DR EMBL; K02238; AAA39719.1; JOINED; Genomic_DNA.
DR EMBL; K02239; AAA39719.1; JOINED; Genomic_DNA.
DR EMBL; K02240; AAA39719.1; JOINED; Genomic_DNA.
DR EMBL; X12973; CAA31416.1; -; Genomic_DNA.
DR CCDS; CCDS15024.1; -. [P05977-1]
DR CCDS; CCDS48284.1; -. [P05977-2]
DR PIR; A23253; A23253.
DR PIR; B23253; B23253.
DR RefSeq; NP_001106858.1; NM_001113387.1. [P05977-2]
DR RefSeq; NP_067260.1; NM_021285.3. [P05977-1]
DR RefSeq; XP_006495826.1; XM_006495763.1. [P05977-1]
DR PDB; 7NEP; EM; 10.20 A; N/O=40-187.
DR PDB; 7QIO; EM; 9.00 A; N/O=1-188.
DR PDBsum; 7NEP; -.
DR PDBsum; 7QIO; -.
DR AlphaFoldDB; P05977; -.
DR SMR; P05977; -.
DR BioGRID; 201656; 5.
DR IntAct; P05977; 4.
DR MINT; P05977; -.
DR STRING; 10090.ENSMUSP00000027151; -.
DR iPTMnet; P05977; -.
DR PhosphoSitePlus; P05977; -.
DR SWISS-2DPAGE; P05977; -.
DR UCD-2DPAGE; P05977; -.
DR PaxDb; P05977; -.
DR PeptideAtlas; P05977; -.
DR PRIDE; P05977; -.
DR ProteomicsDB; 287529; -. [P05977-1]
DR ProteomicsDB; 287530; -. [P05977-2]
DR Antibodypedia; 20019; 221 antibodies from 26 providers.
DR DNASU; 17901; -.
DR Ensembl; ENSMUST00000027151; ENSMUSP00000027151; ENSMUSG00000061816. [P05977-1]
DR Ensembl; ENSMUST00000119429; ENSMUSP00000112861; ENSMUSG00000061816. [P05977-2]
DR GeneID; 17901; -.
DR KEGG; mmu:17901; -.
DR UCSC; uc007bit.2; mouse. [P05977-1]
DR CTD; 4632; -.
DR MGI; MGI:97269; Myl1.
DR VEuPathDB; HostDB:ENSMUSG00000061816; -.
DR eggNOG; KOG0030; Eukaryota.
DR GeneTree; ENSGT01030000234570; -.
DR HOGENOM; CLU_061288_13_0_1; -.
DR InParanoid; P05977; -.
DR OMA; VAKHDDK; -.
DR PhylomeDB; P05977; -.
DR TreeFam; TF351553; -.
DR Reactome; R-MMU-390522; Striated Muscle Contraction.
DR BioGRID-ORCS; 17901; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Myl1; mouse.
DR PRO; PR:P05977; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P05977; protein.
DR Bgee; ENSMUSG00000061816; Expressed in quadriceps femoris and 83 other tissues.
DR ExpressionAtlas; P05977; baseline and differential.
DR Genevisible; P05977; MM.
DR GO; GO:0043292; C:contractile fiber; ISO:MGI.
DR GO; GO:0030016; C:myofibril; ISO:MGI.
DR GO; GO:0016460; C:myosin II complex; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0008307; F:structural constituent of muscle; ISS:UniProtKB.
DR GO; GO:0060048; P:cardiac muscle contraction; ISO:MGI.
DR GO; GO:0006936; P:muscle contraction; ISO:MGI.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; Methylation;
KW Motor protein; Muscle protein; Myosin; Phosphoprotein; Reference proteome;
KW Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P02602"
FT CHAIN 2..188
FT /note="Myosin light chain 1/3, skeletal muscle isoform"
FT /id="PRO_0000198682"
FT DOMAIN 44..79
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 121..156
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 156..188
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N,N,N-trimethylalanine"
FT /evidence="ECO:0000250|UniProtKB:P02602"
FT MOD_RES 65
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P02600"
FT MOD_RES 67
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P02600"
FT MOD_RES 81
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P02600"
FT MOD_RES 93
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P02600"
FT VAR_SEQ 1..47
FT /note="MAPKKDVKKPAAAPAPAPAPAPAPAKPKEEKIDLSAIKIEFSKEQQE -> M
FT SFSADQIA (in isoform MLC3)"
FT /evidence="ECO:0000305"
FT /id="VSP_038687"
FT INIT_MET P05977-2:1
FT /note="Removed"
FT /evidence="ECO:0000305"
FT MOD_RES P05977-2:2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000305"
SQ SEQUENCE 188 AA; 20595 MW; 84C580DA22E25217 CRC64;
MAPKKDVKKP AAAPAPAPAP APAPAKPKEE KIDLSAIKIE FSKEQQEDFK EAFLLFDRTG
ECKITLSQVG DVLRALGTNP TNAEVKKVLG NPSNEEMNAK KIEFEQFLPM MQAISNNKDQ
GGYEDFVEGL RVFDKEGNGT VMGAELRHVL ATLGEKMKEE EVEALLAGQE DSNGCINYEA
FVKHIMSV
