MYL3_BOVIN
ID MYL3_BOVIN Reviewed; 199 AA.
AC P85100;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Myosin light chain 3 {ECO:0000305};
DE AltName: Full=Myosin light chain 1, slow-twitch muscle B/ventricular isoform {ECO:0000250|UniProtKB:P08590};
DE Short=MLC1SB {ECO:0000250|UniProtKB:P08590};
GN Name=MYL3 {ECO:0000250|UniProtKB:P08590};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford;
RX PubMed=19390049; DOI=10.1126/science.1169588;
RG The bovine genome sequencing and analysis consortium;
RT "The genome sequence of taurine cattle: a window to ruminant biology and
RT evolution.";
RL Science 324:522-528(2009).
RN [2] {ECO:0000305}
RP METHYLATION AT ALA-2.
RX PubMed=3979397; DOI=10.1111/j.1432-1033.1985.tb08809.x;
RA Henry G.D., Trayer I.P., Brewer S., Levine B.A.;
RT "The widespread distribution of alpha-N-trimethylalanine as the N-terminal
RT amino acid of light chains from vertebrate striated muscle myosins.";
RL Eur. J. Biochem. 148:75-82(1985).
CC -!- FUNCTION: Regulatory light chain of myosin. Does not bind calcium.
CC {ECO:0000305}.
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC -!- PTM: N-terminus is methylated by METTL11A/NTM1. {ECO:0000250}.
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DR EMBL; AAFC03056301; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001069969.2; NM_001076501.2.
DR PDB; 5N69; X-ray; 2.45 A; G/H=1-199.
DR PDB; 6FSA; X-ray; 2.33 A; D/H=1-199.
DR PDBsum; 5N69; -.
DR PDBsum; 6FSA; -.
DR AlphaFoldDB; P85100; -.
DR SMR; P85100; -.
DR STRING; 9913.ENSBTAP00000011047; -.
DR iPTMnet; P85100; -.
DR PaxDb; P85100; -.
DR PRIDE; P85100; -.
DR GeneID; 618352; -.
DR KEGG; bta:618352; -.
DR CTD; 4634; -.
DR eggNOG; KOG0030; Eukaryota.
DR HOGENOM; CLU_061288_13_0_1; -.
DR InParanoid; P85100; -.
DR OrthoDB; 1470794at2759; -.
DR TreeFam; TF351553; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0031672; C:A band; IBA:GO_Central.
DR GO; GO:0016460; C:myosin II complex; IBA:GO_Central.
DR GO; GO:0003785; F:actin monomer binding; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0060048; P:cardiac muscle contraction; IBA:GO_Central.
DR GO; GO:0055010; P:ventricular cardiac muscle tissue morphogenesis; IBA:GO_Central.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Methylation; Motor protein; Muscle protein; Myosin;
KW Phosphoprotein; Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P09542"
FT CHAIN 2..199
FT /note="Myosin light chain 3"
FT /id="PRO_0000283743"
FT DOMAIN 53..90
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 132..167
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 167..199
FT /note="EF-hand 3"
FT /evidence="ECO:0000305"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..37
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N,N,N-trimethylalanine"
FT /evidence="ECO:0000269|PubMed:3979397"
FT MOD_RES 92
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P16409"
FT MOD_RES 131
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P09542"
FT MOD_RES 133
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P16409"
FT MOD_RES 134
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:P16409"
FT MOD_RES 183
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P09542"
FT HELIX 43..45
FT /evidence="ECO:0007829|PDB:5N69"
FT HELIX 52..64
FT /evidence="ECO:0007829|PDB:6FSA"
FT STRAND 73..76
FT /evidence="ECO:0007829|PDB:5N69"
FT TURN 77..79
FT /evidence="ECO:0007829|PDB:6FSA"
FT HELIX 80..86
FT /evidence="ECO:0007829|PDB:6FSA"
FT HELIX 93..99
FT /evidence="ECO:0007829|PDB:6FSA"
FT TURN 105..109
FT /evidence="ECO:0007829|PDB:6FSA"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:5N69"
FT HELIX 115..126
FT /evidence="ECO:0007829|PDB:6FSA"
FT HELIX 134..142
FT /evidence="ECO:0007829|PDB:6FSA"
FT STRAND 146..150
FT /evidence="ECO:0007829|PDB:6FSA"
FT HELIX 154..163
FT /evidence="ECO:0007829|PDB:6FSA"
FT STRAND 164..166
FT /evidence="ECO:0007829|PDB:6FSA"
FT HELIX 170..176
FT /evidence="ECO:0007829|PDB:6FSA"
FT TURN 177..179
FT /evidence="ECO:0007829|PDB:6FSA"
FT HELIX 191..196
FT /evidence="ECO:0007829|PDB:6FSA"
SQ SEQUENCE 199 AA; 21939 MW; BC05C19146FD964C CRC64;
MAPKKPDPKK DEAKAGAKAA AAPAPAPAPP PAPEPSKEPE FDPSKIKIEF TPEQIEEFKE
AFTLFDRTPK CEMKITYGQC GDVLRALGQN PTQAEVLRVL GKPKQEELNS KMMDFDTFLP
MLQHISKNKD TGTYEDFVEG LRVFDKEGNG TVMGAELRHV LATLGEKLTE DEVEKLMAGQ
EDSNGCINYE AFVKHIMAG
