MYL3_RAT
ID MYL3_RAT Reviewed; 200 AA.
AC P16409;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Myosin light chain 3 {ECO:0000305};
DE AltName: Full=Myosin alkali light chain 1, ventricular {ECO:0000305|PubMed:2798124};
DE Short=MLClV {ECO:0000303|PubMed:2798124};
DE AltName: Full=Myosin light chain 1, slow-twitch muscle B/ventricular isoform {ECO:0000250|UniProtKB:P08590};
DE Short=MLC1SB {ECO:0000250|UniProtKB:P08590};
DE AltName: Full=Ventricular myosin light chain 1 {ECO:0000303|PubMed:2717409};
DE Short=rVMLC1 {ECO:0000303|PubMed:2717409};
GN Name=Myl3 {ECO:0000312|RGD:3142}; Synonyms=Mlc1v {ECO:0000312|RGD:3142};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Heart ventricle;
RX PubMed=2717409; DOI=10.1093/nar/17.7.2753;
RA McNally E., Buttrick P., Leinwand L.;
RT "Ventricular myosin light chain 1 is developmentally regulated and does not
RT change in hypertension.";
RL Nucleic Acids Res. 17:2753-2767(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=Wistar; TISSUE=Heart ventricle;
RX PubMed=2798124; DOI=10.1093/nar/17.19.7723;
RA Periasamy M., Wodgaonkar R., Kumar C., Martin B.J., Siddiqui M.A.Q.;
RT "Characterization of a rat myosin alkali light chain gene expressed in
RT ventricular and slow twitch skeletal muscles.";
RL Nucleic Acids Res. 17:7723-7734(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-93; THR-134; TYR-135 AND
RP SER-184, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Regulatory light chain of myosin. Does not bind calcium.
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC -!- TISSUE SPECIFICITY: Expressed only in ventricular and slow twitch
CC skeletal muscle tissues. {ECO:0000269|PubMed:2798124}.
CC -!- PTM: N-terminus is methylated by METTL11A/NTM1. {ECO:0000250}.
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DR EMBL; X14812; CAA32917.1; -; mRNA.
DR EMBL; X16325; CAA34388.1; -; Genomic_DNA.
DR EMBL; X16326; CAA34388.1; JOINED; Genomic_DNA.
DR EMBL; X16327; CAA34388.1; JOINED; Genomic_DNA.
DR EMBL; X16329; CAA34388.1; JOINED; Genomic_DNA.
DR EMBL; X16330; CAA34388.1; JOINED; Genomic_DNA.
DR EMBL; BC081832; AAH81832.1; -; mRNA.
DR PIR; S09573; MORT3V.
DR RefSeq; NP_036738.1; NM_012606.2.
DR RefSeq; XP_006243979.3; XM_006243917.3.
DR AlphaFoldDB; P16409; -.
DR SMR; P16409; -.
DR BioGRID; 246728; 2.
DR IntAct; P16409; 1.
DR MINT; P16409; -.
DR STRING; 10116.ENSRNOP00000028458; -.
DR iPTMnet; P16409; -.
DR PhosphoSitePlus; P16409; -.
DR jPOST; P16409; -.
DR PaxDb; P16409; -.
DR PRIDE; P16409; -.
DR Ensembl; ENSRNOT00000028458; ENSRNOP00000028458; ENSRNOG00000020955.
DR GeneID; 24585; -.
DR KEGG; rno:24585; -.
DR CTD; 4634; -.
DR RGD; 3142; Myl3.
DR eggNOG; KOG0030; Eukaryota.
DR GeneTree; ENSGT01030000234570; -.
DR HOGENOM; CLU_061288_13_0_1; -.
DR InParanoid; P16409; -.
DR OMA; YDRTPKC; -.
DR OrthoDB; 1470794at2759; -.
DR PhylomeDB; P16409; -.
DR TreeFam; TF351553; -.
DR Reactome; R-RNO-390522; Striated Muscle Contraction.
DR PRO; PR:P16409; -.
DR Proteomes; UP000002494; Chromosome 8.
DR Bgee; ENSRNOG00000020955; Expressed in heart and 17 other tissues.
DR Genevisible; P16409; RN.
DR GO; GO:0031672; C:A band; ISO:RGD.
DR GO; GO:0031674; C:I band; ISO:RGD.
DR GO; GO:0016459; C:myosin complex; IDA:RGD.
DR GO; GO:0016460; C:myosin II complex; IBA:GO_Central.
DR GO; GO:0003785; F:actin monomer binding; ISO:RGD.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0003774; F:cytoskeletal motor activity; IDA:RGD.
DR GO; GO:0060048; P:cardiac muscle contraction; IDA:RGD.
DR GO; GO:0006936; P:muscle contraction; TAS:RGD.
DR GO; GO:0006942; P:regulation of striated muscle contraction; ISO:RGD.
DR GO; GO:0002026; P:regulation of the force of heart contraction; ISO:RGD.
DR GO; GO:0007519; P:skeletal muscle tissue development; IEP:RGD.
DR GO; GO:0055010; P:ventricular cardiac muscle tissue morphogenesis; ISO:RGD.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW Methylation; Motor protein; Muscle protein; Myosin; Phosphoprotein;
KW Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P09542"
FT CHAIN 2..200
FT /note="Myosin light chain 3"
FT /id="PRO_0000198698"
FT DOMAIN 54..91
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 133..168
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 168..200
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N,N,N-trimethylalanine"
FT /evidence="ECO:0000250|UniProtKB:P09542"
FT MOD_RES 93
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 132
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P09542"
FT MOD_RES 134
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 135
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 184
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 200 AA; 22156 MW; 2B8C832A9D364253 CRC64;
MAPKKPEPKK DDAKTAAPKA APAPAAAPAA APEPERPKEA EFDASKIKIE FTPEQIEEFK
EAFQLFDRTP KGEMKITYGQ CGDVLRALGQ NPTQAEVLRV LGKPKQEELN SKMMDFETFL
PMLQHISKNK DTGTYEDFVE GLRVFDKEGN GTVMGAELRH VLATLGERLT EDEVEKLMAG
QEDSNGCINY EAFVKHIMAS
