MYL4_MOUSE
ID MYL4_MOUSE Reviewed; 193 AA.
AC P09541;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Myosin light chain 4;
DE AltName: Full=MLC1EMB;
DE AltName: Full=Myosin light chain 1, atrial/fetal isoform;
DE Short=MLC1A;
GN Name=Myl4; Synonyms=Mlc1a, Myla;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=C3H/HeJ;
RX PubMed=2842339; DOI=10.1016/s0021-9258(18)37805-0;
RA Barton P.J.R., Robert B., Cohen A., Garner I., Sassoon D., Weydert A.,
RA Buckingham M.E.;
RT "Structure and sequence of the myosin alkali light chain gene expressed in
RT adult cardiac atria and fetal striated muscle.";
RL J. Biol. Chem. 263:12669-12676(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-41.
RC STRAIN=C3H/HeJ;
RX PubMed=3194193; DOI=10.1093/nar/16.21.10037;
RA Cohen A., Barton P.J.R., Robert B., Garner I., Alonso S., Buckingham M.E.;
RT "Promoter analysis of myosin alkali light chain genes expressed in mouse
RT striated muscle.";
RL Nucleic Acids Res. 16:10037-10052(1988).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Regulatory light chain of myosin. Does not bind calcium.
CC {ECO:0000305}.
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC -!- TISSUE SPECIFICITY: Expressed in atrial muscle and in fetal skeletal
CC and ventricular muscle.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M20772; AAA39721.1; -; Genomic_DNA.
DR EMBL; M31017; AAA39721.1; JOINED; Genomic_DNA.
DR EMBL; M20769; AAA39721.1; JOINED; Genomic_DNA.
DR EMBL; M20770; AAA39721.1; JOINED; Genomic_DNA.
DR EMBL; M20771; AAA39721.1; JOINED; Genomic_DNA.
DR EMBL; M19436; AAA39720.1; -; mRNA.
DR EMBL; X12971; CAA31414.1; -; Genomic_DNA.
DR CCDS; CCDS25535.1; -.
DR PIR; A31114; MOMS4E.
DR AlphaFoldDB; P09541; -.
DR SMR; P09541; -.
DR IntAct; P09541; 2.
DR MINT; P09541; -.
DR STRING; 10090.ENSMUSP00000018800; -.
DR iPTMnet; P09541; -.
DR PhosphoSitePlus; P09541; -.
DR MaxQB; P09541; -.
DR PaxDb; P09541; -.
DR PeptideAtlas; P09541; -.
DR PRIDE; P09541; -.
DR ProteomicsDB; 287531; -.
DR MGI; MGI:97267; Myl4.
DR eggNOG; KOG0030; Eukaryota.
DR InParanoid; P09541; -.
DR PhylomeDB; P09541; -.
DR Reactome; R-MMU-390522; Striated Muscle Contraction.
DR ChiTaRS; Myl4; mouse.
DR PRO; PR:P09541; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P09541; protein.
DR GO; GO:0031672; C:A band; ISO:MGI.
DR GO; GO:0016460; C:myosin II complex; IBA:GO_Central.
DR GO; GO:0051015; F:actin filament binding; ISO:MGI.
DR GO; GO:0003785; F:actin monomer binding; ISO:MGI.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0060048; P:cardiac muscle contraction; ISO:MGI.
DR GO; GO:0032781; P:positive regulation of ATP-dependent activity; ISO:MGI.
DR GO; GO:0002026; P:regulation of the force of heart contraction; ISO:MGI.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Methylation; Motor protein; Muscle protein; Myosin; Reference proteome;
KW Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:F1RRT2"
FT CHAIN 2..193
FT /note="Myosin light chain 4"
FT /id="PRO_0000198700"
FT DOMAIN 47..84
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 126..161
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N,N-dimethylproline"
FT /evidence="ECO:0000250|UniProtKB:F1RRT2"
SQ SEQUENCE 193 AA; 21159 MW; 10EC54C72F0E6C5E CRC64;
MPPKKPEPKK EAAKPAAAPA PAASAAPEPL KDSAFDPKSV KIDFSADQIE EFKEAFSLFD
RTPTGEMKIT YGQCGDVLRA LGQNPTNAEV LRVLGKPKPE EMSSKTLDFE MFLPILQHIS
RNKEQGTYED FVEGLRVFDK ESNGTVMGAE LRHVLATLGE KMSEAEVEQL LSGQEDANGC
INYEAFVKHI MSG
