ID   MYL4_PIG                Reviewed;         197 AA.
AC   F1RRT2;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2016, sequence version 3.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Myosin light chain 4 {ECO:0000250|UniProtKB:P12829};
DE   AltName: Full=Myosin essential light chain, atrial isoform {ECO:0000303|Ref.2};
GN   Name=MYL4;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823 {ECO:0000312|Proteomes:UP000008227};
RN   [1] {ECO:0000312|Proteomes:UP000008227}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Duroc;
RG   Porcine genome sequencing project;
RL   Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 2-192, METHYLATION AT PRO-2, MASS SPECTROMETRY, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RA   Gregorich Z.R., Cai W., Chen A.J., Peng Y., Ge Y.;
RT   "Distinct sequences and post-translational modifications in cardiac atrial
RT   and ventricular myosin light chains revealed by top-down mass
RT   spectrometry.";
RL   Submitted (JUL-2016) to UniProtKB.
CC   -!- FUNCTION: Regulatory light chain of myosin. Does not bind calcium.
CC       {ECO:0000305}.
CC   -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC       {ECO:0000305}.
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DR   EMBL; CU914417; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; XP_003131354.1; XM_003131306.2.
DR   RefSeq; XP_013845371.1; XM_013989917.1.
DR   AlphaFoldDB; F1RRT2; -.
DR   SMR; F1RRT2; -.
DR   STRING; 9823.ENSSSCP00000018340; -.
DR   PaxDb; F1RRT2; -.
DR   PeptideAtlas; F1RRT2; -.
DR   PRIDE; F1RRT2; -.
DR   Ensembl; ENSSSCT00000018843; ENSSSCP00000018340; ENSSSCG00000017307.
DR   Ensembl; ENSSSCT00005070782; ENSSSCP00005044122; ENSSSCG00005044015.
DR   Ensembl; ENSSSCT00015011014; ENSSSCP00015004331; ENSSSCG00015008360.
DR   Ensembl; ENSSSCT00025035691; ENSSSCP00025014896; ENSSSCG00025026384.
DR   Ensembl; ENSSSCT00030013327; ENSSSCP00030005992; ENSSSCG00030009718.
DR   Ensembl; ENSSSCT00035028070; ENSSSCP00035010797; ENSSSCG00035021514.
DR   Ensembl; ENSSSCT00040043948; ENSSSCP00040018459; ENSSSCG00040032634.
DR   Ensembl; ENSSSCT00045064805; ENSSSCP00045045811; ENSSSCG00045037542.
DR   Ensembl; ENSSSCT00050086525; ENSSSCP00050037132; ENSSSCG00050063538.
DR   Ensembl; ENSSSCT00055016792; ENSSSCP00055013250; ENSSSCG00055008591.
DR   Ensembl; ENSSSCT00060083219; ENSSSCP00060036070; ENSSSCG00060060985.
DR   Ensembl; ENSSSCT00070014122; ENSSSCP00070011649; ENSSSCG00070007340.
DR   GeneID; 100516998; -.
DR   KEGG; ssc:100516998; -.
DR   CTD; 4635; -.
DR   VGNC; VGNC:90516; MYL4.
DR   eggNOG; KOG0030; Eukaryota.
DR   GeneTree; ENSGT01030000234570; -.
DR   HOGENOM; CLU_061288_13_0_1; -.
DR   InParanoid; F1RRT2; -.
DR   OMA; PSRTMAP; -.
DR   OrthoDB; 1470794at2759; -.
DR   TreeFam; TF351553; -.
DR   Proteomes; UP000008227; Chromosome 12.
DR   Proteomes; UP000314985; Chromosome 12.
DR   Bgee; ENSSSCG00000017307; Expressed in blood and 14 other tissues.
DR   ExpressionAtlas; F1RRT2; differential.
DR   GO; GO:0031672; C:A band; IBA:GO_Central.
DR   GO; GO:0016460; C:myosin II complex; IBA:GO_Central.
DR   GO; GO:0051015; F:actin filament binding; IEA:Ensembl.
DR   GO; GO:0003785; F:actin monomer binding; IBA:GO_Central.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0060048; P:cardiac muscle contraction; IBA:GO_Central.
DR   GO; GO:0032781; P:positive regulation of ATP-dependent activity; IEA:Ensembl.
DR   GO; GO:0002026; P:regulation of the force of heart contraction; IEA:Ensembl.
DR   CDD; cd00051; EFh; 1.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR002048; EF_hand_dom.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS50222; EF_HAND_2; 2.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Methylation; Motor protein; Muscle protein;
KW   Myosin; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|Ref.2"
FT   CHAIN           2..197
FT                   /note="Myosin light chain 4"
FT                   /id="PRO_0000437967"
FT   DOMAIN          56..93
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          135..170
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   REGION          1..43
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        18..37
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         2
FT                   /note="N,N-dimethylproline"
FT                   /evidence="ECO:0000269|Ref.1"
SQ   SEQUENCE   197 AA;  21607 MW;  177146CF73DB6633 CRC64;
     MPPKKPEPKK EAAKAAPAPA PAPAPAPAPP PEPAKEPTFD PKSIKIDFTA DQIEEFKEAF
     SLFDRTPTGE MKITYGQCGD VLRALGQNPT NAEVLRVLGK PKPEEMNAKM LDFETFLPIL
     QHISRNKEQG TYEDFVEGLR VFDKESNGTV MGAELRHVLA TLGEKMTEAE VEQLLAGQED
     ANGCINYEAF VKHIMSG