MYL6B_HUMAN
ID MYL6B_HUMAN Reviewed; 208 AA.
AC P14649;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 193.
DE RecName: Full=Myosin light chain 6B;
DE AltName: Full=Myosin light chain 1 slow-twitch muscle A isoform;
DE Short=MLC1sa;
DE AltName: Full=Smooth muscle and nonmuscle myosin light chain alkali 6B;
GN Name=MYL6B; Synonyms=MLC1SA;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Skeletal muscle;
RX PubMed=2304459; DOI=10.1128/mcb.10.3.1095-1104.1990;
RA Hailstones D.L., Gunning P.W.;
RT "Characterization of human myosin light chains 1sa and 3nm: implications
RT for isoform evolution and function.";
RL Mol. Cell. Biol. 10:1095-1104(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Skeletal muscle;
RX PubMed=2602161; DOI=10.1093/nar/17.24.10496;
RA Zimmermann K., Starzinski-Powitz A.;
RT "A novel isoform of myosin alkali light chain isolated from human muscle
RT cells.";
RL Nucleic Acids Res. 17:10496-10496(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Regulatory light chain of myosin. Does not bind calcium.
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC -!- INTERACTION:
CC P14649; Q15506: SPA17; NbExp=12; IntAct=EBI-358570, EBI-1377865;
CC P14649; Q02440: MYO5A; Xeno; NbExp=2; IntAct=EBI-358570, EBI-1040586;
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DR EMBL; X16434; CAA34457.1; -; mRNA.
DR EMBL; M31211; AAA36320.1; -; mRNA.
DR EMBL; BC012425; AAH12425.1; -; mRNA.
DR EMBL; BC014400; AAH14400.1; -; mRNA.
DR CCDS; CCDS8905.1; -.
DR PIR; A34757; MOHUSA.
DR RefSeq; NP_001186558.1; NM_001199629.1.
DR RefSeq; NP_002466.1; NM_002475.4.
DR PDB; 1OE9; X-ray; 2.05 A; B=59-208.
DR PDB; 1W7I; X-ray; 3.00 A; B=59-208.
DR PDB; 1W7J; X-ray; 2.00 A; B=59-208.
DR PDB; 7PLT; EM; 3.30 A; B=58-208.
DR PDB; 7PLU; EM; 3.20 A; B/E=58-208.
DR PDB; 7PLV; EM; 3.50 A; B=58-208.
DR PDB; 7PLW; EM; 3.50 A; B=58-208.
DR PDB; 7PLX; EM; 3.60 A; B=58-208.
DR PDB; 7PLY; EM; 3.20 A; B=58-208.
DR PDB; 7PLZ; EM; 3.20 A; B/E=58-208.
DR PDB; 7PM0; EM; 3.60 A; B=58-208.
DR PDB; 7PM1; EM; 3.50 A; B=58-208.
DR PDB; 7PM2; EM; 3.60 A; B=58-208.
DR PDB; 7PM5; EM; 3.10 A; B=58-208.
DR PDB; 7PM6; EM; 3.00 A; B/E=58-208.
DR PDB; 7PM7; EM; 3.50 A; B=58-208.
DR PDB; 7PM8; EM; 3.50 A; B=58-208.
DR PDB; 7PM9; EM; 3.70 A; B=58-208.
DR PDB; 7PMA; EM; 3.60 A; B=58-208.
DR PDB; 7PMB; EM; 3.60 A; B=58-208.
DR PDB; 7PMC; EM; 3.70 A; B=58-208.
DR PDB; 7PMD; EM; 2.90 A; B=58-208.
DR PDB; 7PME; EM; 2.90 A; B/E=58-208.
DR PDB; 7PMF; EM; 3.40 A; B=58-208.
DR PDB; 7PMG; EM; 3.30 A; B=58-208.
DR PDB; 7PMH; EM; 3.40 A; B=58-208.
DR PDB; 7PMI; EM; 3.30 A; B=58-208.
DR PDB; 7PMJ; EM; 3.40 A; B=58-208.
DR PDB; 7PML; EM; 3.30 A; B=58-208.
DR PDBsum; 1OE9; -.
DR PDBsum; 1W7I; -.
DR PDBsum; 1W7J; -.
DR PDBsum; 7PLT; -.
DR PDBsum; 7PLU; -.
DR PDBsum; 7PLV; -.
DR PDBsum; 7PLW; -.
DR PDBsum; 7PLX; -.
DR PDBsum; 7PLY; -.
DR PDBsum; 7PLZ; -.
DR PDBsum; 7PM0; -.
DR PDBsum; 7PM1; -.
DR PDBsum; 7PM2; -.
DR PDBsum; 7PM5; -.
DR PDBsum; 7PM6; -.
DR PDBsum; 7PM7; -.
DR PDBsum; 7PM8; -.
DR PDBsum; 7PM9; -.
DR PDBsum; 7PMA; -.
DR PDBsum; 7PMB; -.
DR PDBsum; 7PMC; -.
DR PDBsum; 7PMD; -.
DR PDBsum; 7PME; -.
DR PDBsum; 7PMF; -.
DR PDBsum; 7PMG; -.
DR PDBsum; 7PMH; -.
DR PDBsum; 7PMI; -.
DR PDBsum; 7PMJ; -.
DR PDBsum; 7PML; -.
DR AlphaFoldDB; P14649; -.
DR SMR; P14649; -.
DR BioGRID; 126617; 90.
DR IntAct; P14649; 32.
DR MINT; P14649; -.
DR STRING; 9606.ENSP00000450385; -.
DR DrugBank; DB08378; 4-[4-(2,5-DIOXO-PYRROLIDIN-1-YL)-PHENYLAMINO]-4-HYDROXY-BUTYRIC ACID.
DR iPTMnet; P14649; -.
DR PhosphoSitePlus; P14649; -.
DR SwissPalm; P14649; -.
DR BioMuta; MYL6B; -.
DR DMDM; 127153; -.
DR EPD; P14649; -.
DR jPOST; P14649; -.
DR MassIVE; P14649; -.
DR MaxQB; P14649; -.
DR PaxDb; P14649; -.
DR PeptideAtlas; P14649; -.
DR PRIDE; P14649; -.
DR ProteomicsDB; 53069; -.
DR Antibodypedia; 27985; 191 antibodies from 26 providers.
DR DNASU; 140465; -.
DR Ensembl; ENST00000550443.5; ENSP00000446643.1; ENSG00000196465.10.
DR Ensembl; ENST00000553066.5; ENSP00000450385.1; ENSG00000196465.10.
DR GeneID; 140465; -.
DR KEGG; hsa:140465; -.
DR UCSC; uc001sjt.4; human.
DR CTD; 140465; -.
DR DisGeNET; 140465; -.
DR GeneCards; MYL6B; -.
DR HGNC; HGNC:29823; MYL6B.
DR HPA; ENSG00000196465; Tissue enhanced (skeletal muscle, tongue).
DR MIM; 609930; gene.
DR neXtProt; NX_P14649; -.
DR OpenTargets; ENSG00000196465; -.
DR PharmGKB; PA144596407; -.
DR VEuPathDB; HostDB:ENSG00000196465; -.
DR eggNOG; KOG0030; Eukaryota.
DR GeneTree; ENSGT01030000234570; -.
DR HOGENOM; CLU_061288_13_0_1; -.
DR InParanoid; P14649; -.
DR OMA; DLSKVVX; -.
DR OrthoDB; 1470794at2759; -.
DR PhylomeDB; P14649; -.
DR TreeFam; TF351553; -.
DR PathwayCommons; P14649; -.
DR Reactome; R-HSA-445355; Smooth Muscle Contraction.
DR SignaLink; P14649; -.
DR SIGNOR; P14649; -.
DR BioGRID-ORCS; 140465; 9 hits in 1076 CRISPR screens.
DR ChiTaRS; MYL6B; human.
DR EvolutionaryTrace; P14649; -.
DR GeneWiki; MYL6B; -.
DR GenomeRNAi; 140465; -.
DR Pharos; P14649; Tbio.
DR PRO; PR:P14649; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; P14649; protein.
DR Bgee; ENSG00000196465; Expressed in gluteal muscle and 200 other tissues.
DR ExpressionAtlas; P14649; baseline and differential.
DR Genevisible; P14649; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005859; C:muscle myosin complex; TAS:ProtInc.
DR GO; GO:0016459; C:myosin complex; TAS:HGNC-UCL.
DR GO; GO:0016460; C:myosin II complex; IBA:GO_Central.
DR GO; GO:0016461; C:unconventional myosin complex; TAS:BHF-UCL.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0003774; F:cytoskeletal motor activity; TAS:HGNC-UCL.
DR GO; GO:0008307; F:structural constituent of muscle; IBA:GO_Central.
DR GO; GO:0006936; P:muscle contraction; TAS:HGNC-UCL.
DR GO; GO:0030049; P:muscle filament sliding; TAS:HGNC-UCL.
DR GO; GO:0007519; P:skeletal muscle tissue development; TAS:HGNC-UCL.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Motor protein; Muscle protein; Myosin; Reference proteome;
KW Repeat.
FT CHAIN 1..208
FT /note="Myosin light chain 6B"
FT /id="PRO_0000198695"
FT DOMAIN 64..99
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 141..176
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 176..208
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 69..76
FT /evidence="ECO:0007829|PDB:1W7J"
FT STRAND 79..86
FT /evidence="ECO:0007829|PDB:1W7J"
FT HELIX 89..95
FT /evidence="ECO:0007829|PDB:1W7J"
FT HELIX 102..108
FT /evidence="ECO:0007829|PDB:1W7J"
FT HELIX 114..117
FT /evidence="ECO:0007829|PDB:1W7J"
FT STRAND 121..123
FT /evidence="ECO:0007829|PDB:1W7J"
FT HELIX 124..134
FT /evidence="ECO:0007829|PDB:1W7J"
FT HELIX 147..151
FT /evidence="ECO:0007829|PDB:1W7J"
FT STRAND 157..162
FT /evidence="ECO:0007829|PDB:1W7J"
FT HELIX 163..172
FT /evidence="ECO:0007829|PDB:1W7J"
FT STRAND 173..175
FT /evidence="ECO:0007829|PDB:1W7J"
FT HELIX 179..186
FT /evidence="ECO:0007829|PDB:1W7J"
FT TURN 187..189
FT /evidence="ECO:0007829|PDB:1OE9"
FT STRAND 194..197
FT /evidence="ECO:0007829|PDB:1W7J"
FT HELIX 198..204
FT /evidence="ECO:0007829|PDB:1W7J"
SQ SEQUENCE 208 AA; 22764 MW; 6F3DFAC6CCA9877E CRC64;
MPPKKDVPVK KPAGPSISKP AAKPAAAGAP PAKTKAEPAV PQAPQKTQEP PVDLSKVVIE
FNKDQLEEFK EAFELFDRVG DGKILYSQCG DVMRALGQNP TNAEVLKVLG NPKSDELKSR
RVDFETFLPM LQAVAKNRGQ GTYEDYLEGF RVFDKEGNGK VMGAELRHVL TTLGEKMTEE
EVETVLAGHE DSNGCINYEA FLKHILSV
