MYL6_CHICK
ID MYL6_CHICK Reviewed; 151 AA.
AC P02607; P08296;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Myosin light polypeptide 6;
DE AltName: Full=G2 catalytic;
DE AltName: Full=LC17-GI;
DE AltName: Full=LC17-NM;
DE AltName: Full=Myosin light chain alkali smooth-muscle/non-muscle isoforms;
GN Name=MYL6;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP PROTEIN SEQUENCE (ISOFORM 1).
RX PubMed=7319048; DOI=10.1016/0014-5793(81)80789-2;
RA Matsuda G., Maita T., Kato Y., Chen J., Umegane T.;
RT "Amino acid sequences of the cardiac L-2A, L-2B and gizzard 17 000-Mr light
RT chains of chicken muscle myosin.";
RL FEBS Lett. 135:232-236(1981).
RN [2]
RP PROTEIN SEQUENCE (ISOFORM 1), AND ACETYLATION AT CYS-2.
RX PubMed=6870825; DOI=10.1042/bj2110267;
RA Grand R.J., Perry S.V.;
RT "Preparation of the alkali and P light chains of chicken gizzard myosin.
RT Amino acid sequence of the alkali light chain.";
RL Biochem. J. 211:267-272(1983).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Fibroblast, and Gizzard;
RX PubMed=3038890; DOI=10.1016/s0021-9258(18)61006-3;
RA Nabeshima Y., Nabeshima Y., Nonomura Y., Fujii-Kuriyama Y.;
RT "Nonmuscle and smooth muscle myosin light chain mRNAs are generated from a
RT single gene by the tissue-specific alternative RNA splicing.";
RL J. Biol. Chem. 262:10608-10612(1987).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) (ISOFORM 1).
RX PubMed=9741621; DOI=10.1016/s0092-8674(00)81598-6;
RA Dominguez R., Freyzon Y., Trybus K.M., Cohen C.;
RT "Crystal structure of a vertebrate smooth muscle myosin motor domain and
RT its complex with the essential light chain: visualization of the pre-power
RT stroke state.";
RL Cell 94:559-571(1998).
CC -!- FUNCTION: Regulatory light chain of myosin. Does not bind calcium.
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC -!- INTERACTION:
CC P02607; P10587: MYH11; NbExp=2; IntAct=EBI-1027073, EBI-1027098;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=Smooth muscle, MLC3sm;
CC IsoId=P02607-1; Sequence=Displayed;
CC Name=2; Synonyms=Non-muscle, MLC3nm;
CC IsoId=P02607-2, P08296-1;
CC Sequence=VSP_017063;
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DR EMBL; M15645; AAA48978.1; -; mRNA.
DR EMBL; M15646; AAA48979.1; -; mRNA.
DR PIR; A28488; MOCHG2.
DR PIR; B28488; MOCH6N.
DR PDB; 1BR1; X-ray; 3.50 A; B/D/F/H=2-151.
DR PDB; 1BR4; X-ray; 3.62 A; B/D/F/H=2-151.
DR PDB; 1I84; EM; 20.00 A; T/W=2-151.
DR PDB; 3DTP; EM; 20.00 A; C/D=2-151.
DR PDB; 3J04; EM; -; C/F=4-151.
DR PDB; 7MF3; EM; 3.40 A; C/F=2-151.
DR PDBsum; 1BR1; -.
DR PDBsum; 1BR4; -.
DR PDBsum; 1I84; -.
DR PDBsum; 3DTP; -.
DR PDBsum; 3J04; -.
DR PDBsum; 7MF3; -.
DR AlphaFoldDB; P02607; -.
DR SMR; P02607; -.
DR IntAct; P02607; 2.
DR iPTMnet; P02607; -.
DR VEuPathDB; HostDB:geneid_100996929; -.
DR InParanoid; P02607; -.
DR OrthoDB; 1470794at2759; -.
DR PhylomeDB; P02607; -.
DR EvolutionaryTrace; P02607; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0042641; C:actomyosin; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005859; C:muscle myosin complex; IMP:CAFA.
DR GO; GO:0016460; C:myosin II complex; IDA:UniProtKB.
DR GO; GO:0097513; C:myosin II filament; IDA:UniProtKB.
DR GO; GO:0043531; F:ADP binding; IMP:CAFA.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0000287; F:magnesium ion binding; IMP:CAFA.
DR GO; GO:0000146; F:microfilament motor activity; ISS:HGNC-UCL.
DR GO; GO:0032036; F:myosin heavy chain binding; IPI:CAFA.
DR GO; GO:0045159; F:myosin II binding; IPI:CAFA.
DR GO; GO:0008307; F:structural constituent of muscle; IMP:CAFA.
DR GO; GO:0031032; P:actomyosin structure organization; IDA:UniProtKB.
DR GO; GO:0030239; P:myofibril assembly; IMP:CAFA.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; Direct protein sequencing;
KW Motor protein; Muscle protein; Myosin; Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:7319048"
FT CHAIN 2..151
FT /note="Myosin light polypeptide 6"
FT /id="PRO_0000198694"
FT DOMAIN 7..42
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 84..119
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 119..151
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 2
FT /note="N-acetylcysteine"
FT /evidence="ECO:0000269|PubMed:6870825"
FT VAR_SEQ 143..151
FT /note="ELVRMVLSG -> AFVRHILSG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:3038890"
FT /id="VSP_017063"
FT CONFLICT 66
FT /note="K -> N (in Ref. 1; AA sequence and 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 101
FT /note="N -> D (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 6..18
FT /evidence="ECO:0007829|PDB:7MF3"
FT STRAND 21..25
FT /evidence="ECO:0007829|PDB:1BR1"
FT HELIX 29..38
FT /evidence="ECO:0007829|PDB:7MF3"
FT HELIX 47..52
FT /evidence="ECO:0007829|PDB:7MF3"
FT HELIX 57..60
FT /evidence="ECO:0007829|PDB:1BR1"
FT HELIX 67..78
FT /evidence="ECO:0007829|PDB:7MF3"
FT HELIX 86..96
FT /evidence="ECO:0007829|PDB:7MF3"
FT STRAND 98..101
FT /evidence="ECO:0007829|PDB:7MF3"
FT HELIX 106..114
FT /evidence="ECO:0007829|PDB:7MF3"
FT STRAND 116..118
FT /evidence="ECO:0007829|PDB:1BR1"
FT HELIX 123..127
FT /evidence="ECO:0007829|PDB:7MF3"
FT STRAND 130..132
FT /evidence="ECO:0007829|PDB:7MF3"
FT STRAND 135..137
FT /evidence="ECO:0007829|PDB:7MF3"
FT HELIX 142..148
FT /evidence="ECO:0007829|PDB:7MF3"
SQ SEQUENCE 151 AA; 16987 MW; 40A6916EC1E90C41 CRC64;
MCDFSEEQTA EFKEAFQLFD RTGDGKILYS QCGDVMRALG QNPTNAEVMK VLGNPKSDEM
NLKTLKFEQF LPMMQTIAKN KDQGCFEDYV EGLRVFDKEG NGTVMGAEIR HVLVTLGEKM
TEEEVEQLVA GHEDSNGCIN YEELVRMVLS G
