MYL6_MOUSE
ID MYL6_MOUSE Reviewed; 151 AA.
AC Q60605;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Myosin light polypeptide 6;
DE AltName: Full=17 kDa myosin light chain;
DE Short=LC17;
DE AltName: Full=Myosin light chain 3;
DE Short=MLC-3;
DE AltName: Full=Myosin light chain alkali 3;
DE Short=Myosin light chain A3;
DE AltName: Full=Smooth muscle and nonmuscle myosin light chain alkali 6;
GN Name=Myl6; Synonyms=Myln;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SMOOTH MUSCLE).
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PROTEIN SEQUENCE OF 2-21 AND 27-119 (ISOFORMS NON-MUSCLE/SMOOTH MUSCLE),
RP PROTEIN SEQUENCE OF 120-146 (ISOFORMS NON-MUSCLE AND SMOOTH MUSCLE),
RP CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT CYS-2, AND IDENTIFICATION
RP BY MASS SPECTROMETRY.
RC TISSUE=Embryonic fibroblast;
RA Sumpton D.P., Sandilands E., Frame M.C., Bienvenut W.V.;
RL Submitted (MAR-2008) to UniProtKB.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 11-151 (ISOFORM NON-MUSCLE).
RX PubMed=7804326;
RA Hailstones D.L., Gunning P.W.;
RT "Regulation of nonmuscle myosin light chain 3 gene expression in response
RT to exogenous MLC3nm mRNA.";
RL Cell. Mol. Biol. Res. 40:53-62(1994).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135 (ISOFORM SMOOTH MUSCLE),
RP AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57, PHOSPHORYLATION [LARGE
RP SCALE ANALYSIS] AT SER-135 (ISOFORM SMOOTH MUSCLE), AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-81, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
RN [7]
RP INTERACTION WITH SPATA6.
RX PubMed=25605924; DOI=10.1073/pnas.1424648112;
RA Yuan S., Stratton C.J., Bao J., Zheng H., Bhetwal B.P., Yanagimachi R.,
RA Yan W.;
RT "Spata6 is required for normal assembly of the sperm connecting piece and
RT tight head-tail conjunction.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:E430-E439(2015).
CC -!- FUNCTION: Regulatory light chain of myosin. Does not bind calcium.
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC Interacts with SPATA6 (PubMed:25605924). {ECO:0000269|PubMed:25605924}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Non-muscle; Synonyms=MLC3nm, LC17A, LC17-nm;
CC IsoId=Q60605-1; Sequence=Displayed;
CC Name=Smooth muscle; Synonyms=MLC3sm, LC17B, LC17-sm;
CC IsoId=Q60605-2; Sequence=VSP_009736;
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BC026760; AAH26760.1; -; mRNA.
DR EMBL; U04443; AAC52278.1; -; mRNA.
DR CCDS; CCDS48728.1; -. [Q60605-2]
DR CCDS; CCDS88112.1; -. [Q60605-1]
DR RefSeq; NP_001304147.1; NM_001317218.1. [Q60605-1]
DR RefSeq; NP_034990.1; NM_010860.4. [Q60605-2]
DR AlphaFoldDB; Q60605; -.
DR SMR; Q60605; -.
DR BioGRID; 201658; 18.
DR IntAct; Q60605; 5.
DR MINT; Q60605; -.
DR STRING; 10090.ENSMUSP00000128803; -.
DR iPTMnet; Q60605; -.
DR PhosphoSitePlus; Q60605; -.
DR SwissPalm; Q60605; -.
DR REPRODUCTION-2DPAGE; Q60605; -.
DR UCD-2DPAGE; Q60605; -.
DR CPTAC; non-CPTAC-3845; -.
DR EPD; Q60605; -.
DR jPOST; Q60605; -.
DR MaxQB; Q60605; -.
DR PaxDb; Q60605; -.
DR PeptideAtlas; Q60605; -.
DR PRIDE; Q60605; -.
DR ProteomicsDB; 287532; -. [Q60605-1]
DR ProteomicsDB; 287533; -. [Q60605-2]
DR TopDownProteomics; Q60605-2; -. [Q60605-2]
DR Antibodypedia; 27997; 350 antibodies from 29 providers.
DR DNASU; 17904; -.
DR Ensembl; ENSMUST00000164181; ENSMUSP00000128803; ENSMUSG00000090841. [Q60605-2]
DR Ensembl; ENSMUST00000218127; ENSMUSP00000151693; ENSMUSG00000090841. [Q60605-1]
DR GeneID; 17904; -.
DR KEGG; mmu:17904; -.
DR UCSC; uc007hnb.1; mouse. [Q60605-1]
DR CTD; 4637; -.
DR MGI; MGI:109318; Myl6.
DR VEuPathDB; HostDB:ENSMUSG00000090841; -.
DR eggNOG; KOG0030; Eukaryota.
DR GeneTree; ENSGT01030000234570; -.
DR HOGENOM; CLU_061288_13_0_1; -.
DR InParanoid; Q60605; -.
DR OMA; PANEHRN; -.
DR PhylomeDB; Q60605; -.
DR TreeFam; TF351553; -.
DR Reactome; R-MMU-445355; Smooth Muscle Contraction.
DR Reactome; R-MMU-5627123; RHO GTPases activate PAKs.
DR BioGRID-ORCS; 17904; 4 hits in 73 CRISPR screens.
DR ChiTaRS; Myl6; mouse.
DR PRO; PR:Q60605; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q60605; protein.
DR Bgee; ENSMUSG00000090841; Expressed in granulocyte and 236 other tissues.
DR ExpressionAtlas; Q60605; baseline and differential.
DR Genevisible; Q60605; MM.
DR GO; GO:0005903; C:brush border; ISO:MGI.
DR GO; GO:0016459; C:myosin complex; TAS:HGNC-UCL.
DR GO; GO:0016460; C:myosin II complex; IBA:GO_Central.
DR GO; GO:0016461; C:unconventional myosin complex; IDA:UniProtKB.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0003774; F:cytoskeletal motor activity; TAS:HGNC-UCL.
DR GO; GO:0000146; F:microfilament motor activity; ISS:HGNC-UCL.
DR GO; GO:0008307; F:structural constituent of muscle; ISS:HGNC-UCL.
DR GO; GO:0006936; P:muscle contraction; TAS:HGNC-UCL.
DR GO; GO:0030049; P:muscle filament sliding; TAS:HGNC-UCL.
DR GO; GO:0007519; P:skeletal muscle tissue development; TAS:HGNC-UCL.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Direct protein sequencing;
KW Motor protein; Muscle protein; Myosin; Phosphoprotein; Reference proteome;
KW Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|Ref.2"
FT CHAIN 2..151
FT /note="Myosin light polypeptide 6"
FT /id="PRO_0000198691"
FT DOMAIN 7..42
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 84..119
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 119..151
FT /note="EF-hand 3"
FT /evidence="ECO:0000305"
FT MOD_RES 2
FT /note="N-acetylcysteine"
FT /evidence="ECO:0000269|Ref.2"
FT MOD_RES 57
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 81
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT VAR_SEQ 143..151
FT /note="AFVRHILSG -> ELVRMVLNG (in isoform Smooth muscle)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_009736"
FT CONFLICT 54
FT /note="N -> T (in Ref. 3; AAC52278)"
FT /evidence="ECO:0000305"
FT CONFLICT 82
FT /note="D -> A (in Ref. 3; AAC52278)"
FT /evidence="ECO:0000305"
FT MOD_RES Q60605-2:135
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
SQ SEQUENCE 151 AA; 16930 MW; A2B7B4F41179523D CRC64;
MCDFTEDQTA EFKEAFQLFD RTGDGKILYS QCGDVMRALG QNPTNAEVLK VLGNPKSDEM
NVKVLDFEHF LPMLQTVAKN KDQGTYEDYV EGLRVFDKEG NGTVMGAEIR HVLVTLGEKM
TEEEVEMLVA GHEDSNGCIN YEAFVRHILS G
