MYO1A_MOUSE
ID MYO1A_MOUSE Reviewed; 1043 AA.
AC O88329; B2RW65;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Unconventional myosin-Ia;
DE AltName: Full=Brush border myosin I;
DE Short=BBM-I;
DE Short=BBMI;
DE AltName: Full=Myosin I heavy chain;
DE Short=MIHC;
GN Name=Myo1a; Synonyms=Bbmi, Myhl;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-909.
RX PubMed=9933937;
RX DOI=10.1002/(sici)1097-010x(19990215)283:3<242::aid-jez3>3.0.co;2-f;
RA Skowron J.F., Mooseker M.S.;
RT "Cloning and characterization of mouse brush border myosin-I in adult and
RT embryonic intestine.";
RL J. Exp. Zool. 283:242-257(1999).
CC -!- FUNCTION: Involved in directing the movement of organelles along actin
CC filaments. {ECO:0000305}.
CC -!- PTM: Phosphorylated by ALPK1. {ECO:0000250|UniProtKB:Q9UBC5}.
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Myosin family. {ECO:0000305}.
CC -!- CAUTION: Represents an unconventional myosin. This protein should not
CC be confused with the conventional myosin-1 (MYH1). {ECO:0000305}.
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DR EMBL; AC160970; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC147605; AAI47606.1; -; mRNA.
DR EMBL; BC147612; AAI47613.1; -; mRNA.
DR EMBL; AF009960; AAC28397.1; -; mRNA.
DR CCDS; CCDS36083.1; -.
DR RefSeq; NP_001074688.1; NM_001081219.2.
DR RefSeq; XP_006513922.1; XM_006513859.3.
DR RefSeq; XP_006513923.1; XM_006513860.3.
DR AlphaFoldDB; O88329; -.
DR SMR; O88329; -.
DR BioGRID; 240656; 1.
DR IntAct; O88329; 1.
DR STRING; 10090.ENSMUSP00000078540; -.
DR iPTMnet; O88329; -.
DR PhosphoSitePlus; O88329; -.
DR jPOST; O88329; -.
DR MaxQB; O88329; -.
DR PaxDb; O88329; -.
DR PeptideAtlas; O88329; -.
DR PRIDE; O88329; -.
DR ProteomicsDB; 293604; -.
DR Antibodypedia; 28435; 98 antibodies from 24 providers.
DR Ensembl; ENSMUST00000079590; ENSMUSP00000078540; ENSMUSG00000025401.
DR GeneID; 432516; -.
DR KEGG; mmu:432516; -.
DR UCSC; uc007hkh.2; mouse.
DR CTD; 4640; -.
DR MGI; MGI:107732; Myo1a.
DR VEuPathDB; HostDB:ENSMUSG00000025401; -.
DR eggNOG; KOG0164; Eukaryota.
DR GeneTree; ENSGT00940000160660; -.
DR HOGENOM; CLU_000192_7_7_1; -.
DR InParanoid; O88329; -.
DR OMA; IKPNEYQ; -.
DR OrthoDB; 122881at2759; -.
DR PhylomeDB; O88329; -.
DR TreeFam; TF312960; -.
DR BioGRID-ORCS; 432516; 4 hits in 76 CRISPR screens.
DR PRO; PR:O88329; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; O88329; protein.
DR Bgee; ENSMUSG00000025401; Expressed in small intestine Peyer's patch and 61 other tissues.
DR Genevisible; O88329; MM.
DR GO; GO:0015629; C:actin cytoskeleton; ISO:MGI.
DR GO; GO:0016324; C:apical plasma membrane; IDA:MGI.
DR GO; GO:0009925; C:basal plasma membrane; IDA:MGI.
DR GO; GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
DR GO; GO:0005903; C:brush border; IDA:UniProtKB.
DR GO; GO:0031252; C:cell leading edge; ISO:MGI.
DR GO; GO:0030864; C:cortical actin cytoskeleton; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0031941; C:filamentous actin; ISS:UniProtKB.
DR GO; GO:0030426; C:growth cone; ISO:MGI.
DR GO; GO:0016328; C:lateral plasma membrane; IDA:MGI.
DR GO; GO:0045121; C:membrane raft; ISO:MGI.
DR GO; GO:0005902; C:microvillus; IDA:MGI.
DR GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR GO; GO:0043005; C:neuron projection; ISO:MGI.
DR GO; GO:0043025; C:neuronal cell body; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0044853; C:plasma membrane raft; IDA:UniProtKB.
DR GO; GO:0051015; F:actin filament binding; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0000146; F:microfilament motor activity; IBA:GO_Central.
DR GO; GO:0007015; P:actin filament organization; IBA:GO_Central.
DR GO; GO:0030030; P:cell projection organization; IMP:MGI.
DR GO; GO:0030033; P:microvillus assembly; IMP:MGI.
DR GO; GO:0032880; P:regulation of protein localization; ISO:MGI.
DR GO; GO:0007605; P:sensory perception of sound; ISS:UniProtKB.
DR GO; GO:0051648; P:vesicle localization; ISS:UniProtKB.
DR GO; GO:0030050; P:vesicle transport along actin filament; IBA:GO_Central.
DR CDD; cd01378; MYSc_Myo1; 1.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR001609; Myosin_head_motor_dom.
DR InterPro; IPR010926; Myosin_TH1.
DR InterPro; IPR036072; MYSc_Myo1.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00612; IQ; 2.
DR Pfam; PF00063; Myosin_head; 1.
DR Pfam; PF06017; Myosin_TH1; 1.
DR PRINTS; PR00193; MYOSINHEAVY.
DR SMART; SM00015; IQ; 3.
DR SMART; SM00242; MYSc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50096; IQ; 2.
DR PROSITE; PS51456; MYOSIN_MOTOR; 1.
DR PROSITE; PS51757; TH1; 1.
PE 2: Evidence at transcript level;
KW Actin-binding; ATP-binding; Calmodulin-binding; Motor protein; Myosin;
KW Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..1043
FT /note="Unconventional myosin-Ia"
FT /id="PRO_0000123439"
FT DOMAIN 8..694
FT /note="Myosin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00782"
FT DOMAIN 697..719
FT /note="IQ 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT DOMAIN 720..742
FT /note="IQ 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT DOMAIN 743..772
FT /note="IQ 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT DOMAIN 858..1042
FT /note="TH1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01093"
FT REGION 571..593
FT /note="Actin-binding"
FT /evidence="ECO:0000255"
FT BINDING 101..108
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT CONFLICT 397
FT /note="I -> L (in Ref. 3; AAC28397)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1043 AA; 118695 MW; D66000A9E6FFF17E CRC64;
MPLLEGPVGV EDLILLEPLD EESLIKNLQL RYENKEIYTY IGNVVISMNP YEQLPIYGPE
FIAKYRDYTF YELKPHIYAL ANVAYQSLKD RDRDQCILIT GESGAGKTEA SKLVMSYVAA
VCGKGEQVNS VKEQLLQSNP VLEAFGNAKT IRNNNSSRFG KYMDIEFDFK GSPLGGVITN
YLLEKSRVVK QLKGERNFHI FYQLLAGADA QLLKALKLEE DTSVYGYLNG EVSKVNGMDD
ASNFRAVQHA MSVIGFSEEE IRQVLEVTAL VLKLGNVKLT DEFQANGIPA SGICDGKGIQ
EIGEMMGLNS TELERALCSR TMETGKEKVV TVLNVTQAQY ARDALAKNIY SRLFDWIVKR
INESIKVGTG EKKKVMGVLD IYGFEILEDN SFEQFVINYC NERLQQVFIE LTLKEEQEEY
KREGIPWTKV EYFDNGIICN LIEHSQRGIL AMLDEECLRP GVVSDSTFLA KLNQLFSKHS
HYESKVSQNA QRQYDRTMGL SCFRISHYAG KVTYNVTGFI DKNNDLLFRD LSQTMWKAQH
PLLKSLFPEG NPKEASLKRP PTAGTQFKNS VAVLMKNLYS KNPNYIRCIK PNDQQQKGRF
TSEMVMVQAR YLGLLENVRV RRAGYAFRQG YKPFLERYRL LSRSTWPRWN GDDREGVEKV
LGSLTLSSEE LAYGKTKIFI RSPKTLFYLE EQRRLRLQQL ATLIQKVYRG WRCRTHYQQM
RKSQILISAW FRGNKQKKHY GKIRSSVLLI QAFVRGWRAR KNYRKYFRSG AALTLANFIY
QSMAQKFLLN LKKNLPSTKV LDNTWPAAPY RCFNTANQEL QRLFYQWKCK KFRDQLSPKQ
VQTLREKLCA SELFKGKKAS YPQSVPIPFR GDYIGLQGNP KLQRLKGREE GPVLVADTVK
KVNRGNGKTS ARILLLTKGH VILTDAKKSQ AQIVIGLEDV AGVSVSSLQD GLFSLHLSEM
SSAVSKGDIL LVSDHVVELL TKMYQAVLDA TQRQLSVTVT EKFSVRFKEG SVAVKVIQGP
EGGGNRKLIC KKKGSNAMEV TVR
