MYO1_YEAST
ID MYO1_YEAST Reviewed; 1928 AA.
AC P08964; D3DKX0;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 3.
DT 03-AUG-2022, entry version 207.
DE RecName: Full=Myosin-1;
DE AltName: Full=Type II myosin;
GN Name=MYO1; OrderedLocusNames=YHR023W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=2263482; DOI=10.1093/nar/18.23.7147;
RA Sweeney F.P., Watts F.Z., Pocklington M.J., Orr E.;
RT "The MYO1 gene from Saccharomyces cerevisiae: its complete nucleotide
RT sequence.";
RL Nucleic Acids Res. 18:7147-7147(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-760.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=3322809; DOI=10.1002/j.1460-2075.1987.tb02675.x;
RA Watts F.Z., Shiels G., Orr E.;
RT "The yeast MYO1 gene encoding a myosin-like protein required for cell
RT division.";
RL EMBO J. 6:3499-3505(1987).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Required for cell division.
CC -!- INTERACTION:
CC P08964; P53141: MLC1; NbExp=4; IntAct=EBI-11650, EBI-10988;
CC P08964; Q06580: MLC2; NbExp=3; IntAct=EBI-11650, EBI-10999;
CC -!- MISCELLANEOUS: Present with 2140 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Myosin family. {ECO:0000305}.
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DR EMBL; X53947; CAA37894.1; -; Genomic_DNA.
DR EMBL; U10399; AAB68872.1; -; Genomic_DNA.
DR EMBL; X06187; CAA29550.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06714.1; -; Genomic_DNA.
DR PIR; S46773; S46773.
DR RefSeq; NP_011888.1; NM_001179153.1.
DR AlphaFoldDB; P08964; -.
DR SMR; P08964; -.
DR BioGRID; 36454; 233.
DR ComplexPortal; CPX-1426; Myosin class II complex.
DR DIP; DIP-808N; -.
DR IntAct; P08964; 23.
DR MINT; P08964; -.
DR STRING; 4932.YHR023W; -.
DR iPTMnet; P08964; -.
DR MaxQB; P08964; -.
DR PaxDb; P08964; -.
DR PRIDE; P08964; -.
DR EnsemblFungi; YHR023W_mRNA; YHR023W; YHR023W.
DR GeneID; 856418; -.
DR KEGG; sce:YHR023W; -.
DR SGD; S000001065; MYO1.
DR VEuPathDB; FungiDB:YHR023W; -.
DR eggNOG; KOG0161; Eukaryota.
DR GeneTree; ENSGT00940000175738; -.
DR HOGENOM; CLU_000192_5_3_1; -.
DR InParanoid; P08964; -.
DR OMA; NAHNEAQ; -.
DR BioCyc; YEAST:G3O-31084-MON; -.
DR Reactome; R-SCE-5627123; RHO GTPases activate PAKs.
DR PRO; PR:P08964; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P08964; protein.
DR GO; GO:0005935; C:cellular bud neck; IDA:SGD.
DR GO; GO:0000142; C:cellular bud neck contractile ring; IDA:SGD.
DR GO; GO:0000131; C:incipient cellular bud site; IDA:SGD.
DR GO; GO:0110085; C:mitotic actomyosin contractile ring; IBA:GO_Central.
DR GO; GO:0032982; C:myosin filament; IBA:GO_Central.
DR GO; GO:0016460; C:myosin II complex; IPI:SGD.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000146; F:microfilament motor activity; IBA:GO_Central.
DR GO; GO:0032033; F:myosin II light chain binding; IPI:SGD.
DR GO; GO:0032027; F:myosin light chain binding; IPI:SGD.
DR GO; GO:1903475; P:mitotic actomyosin contractile ring assembly; IMP:SGD.
DR GO; GO:1902404; P:mitotic actomyosin contractile ring contraction; IMP:SGD.
DR GO; GO:0031671; P:primary cell septum biogenesis; IMP:SGD.
DR GO; GO:1904498; P:protein localization to mitotic actomyosin contractile ring; IDA:SGD.
DR GO; GO:0000920; P:septum digestion after cytokinesis; IMP:SGD.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR001609; Myosin_head_motor_dom.
DR InterPro; IPR004009; Myosin_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00063; Myosin_head; 1.
DR PRINTS; PR00193; MYOSINHEAVY.
DR SMART; SM00242; MYSc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50096; IQ; 1.
DR PROSITE; PS51456; MYOSIN_MOTOR; 1.
DR PROSITE; PS51844; SH3_LIKE; 1.
PE 1: Evidence at protein level;
KW Actin-binding; ATP-binding; Coiled coil; Motor protein; Myosin;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..1928
FT /note="Myosin-1"
FT /id="PRO_0000123484"
FT DOMAIN 8..71
FT /note="Myosin N-terminal SH3-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01190"
FT DOMAIN 75..791
FT /note="Myosin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00782"
FT DOMAIN 794..823
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 460..529
FT /note="Actin-binding"
FT /evidence="ECO:0000250"
FT REGION 629..657
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 856..1911
FT /evidence="ECO:0000255"
FT BINDING 180..187
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT CONFLICT 36
FT /note="K -> I (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 46
FT /note="I -> T (in Ref. 1; CAA37894 and 4; CAA29550)"
FT /evidence="ECO:0000305"
FT CONFLICT 59
FT /note="V -> S (in Ref. 1; CAA37894 and 4; CAA29550)"
FT /evidence="ECO:0000305"
FT CONFLICT 86
FT /note="L -> F (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 330
FT /note="Missing (in Ref. 1; CAA37894 and 4; CAA29550)"
FT /evidence="ECO:0000305"
FT CONFLICT 343
FT /note="N -> S (in Ref. 1; CAA37894 and 4; CAA29550)"
FT /evidence="ECO:0000305"
FT CONFLICT 421..426
FT /note="QQAKFI -> TKLSSL (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 515
FT /note="D -> S (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 529..535
FT /note="SKGPPTG -> ARGHDR (in Ref. 1; CAA37894 and 4;
FT CAA29550)"
FT /evidence="ECO:0000305"
FT CONFLICT 541
FT /note="D -> V (in Ref. 1; CAA37894 and 4; CAA29550)"
FT /evidence="ECO:0000305"
FT CONFLICT 550..551
FT /note="TD -> LM (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 573
FT /note="R -> A (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 582
FT /note="H -> D (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 588..599
FT /note="EYTVEGWLSKNK -> NTLWKAGYPKT (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 599
FT /note="Missing (in Ref. 4; CAA29550)"
FT /evidence="ECO:0000305"
FT CONFLICT 627..632
FT /note="EKSSSA -> GKNLLVC (in Ref. 1; CAA37894 and 4;
FT CAA29550)"
FT /evidence="ECO:0000305"
FT CONFLICT 695
FT /note="R -> S (in Ref. 1; CAA37894 and 4; CAA29550)"
FT /evidence="ECO:0000305"
FT CONFLICT 736..742
FT /note="ENSTTTT -> RKFNHHD (in Ref. 4; CAA29550)"
FT /evidence="ECO:0000305"
FT CONFLICT 756
FT /note="E -> R (in Ref. 1; CAA37894 and 4; CAA29550)"
FT /evidence="ECO:0000305"
FT CONFLICT 773..784
FT /note="NTKLFFKAGVLA -> ILTVFQKLEYWS (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 793..794
FT /note="KL -> NV (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 896
FT /note="N -> T (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 900
FT /note="N -> NSQITKINTNITETPQSTYIGERPKRVICGN (in Ref. 1;
FT CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 906
FT /note="N -> I (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 911
FT /note="N -> K (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 915..930
FT /note="NESLLNRVKTSSETLQ -> RIAIKILKPAINIT (in Ref. 1;
FT CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 934..939
FT /note="DDLVSE -> MTLFL (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 951..958
FT /note="AQNLEEAH -> RKILKKLD (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1002
FT /note="S -> C (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1049
FT /note="L -> D (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1056
FT /note="C -> S (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1060
FT /note="M -> I (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1085
FT /note="A -> E (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1123
FT /note="V -> C (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1133
FT /note="L -> S (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1144..1146
FT /note="KSN -> NLI (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1159..1168
FT /note="RETKEQEQKK -> TRKKEEQDKE (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1179..1204
FT /note="SKIKELEARLSQEISLNQYLNKRISG -> ELKVKEWKARCHRKYLKSILKQ
FT KNIR (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1224
FT /note="P -> S (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1228
FT /note="E -> Q (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1253
FT /note="E -> Q (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1311..1323
FT /note="PDKESDINKLMLE -> LTKSLILTNGNAS (in Ref. 1;
FT CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1400
FT /note="D -> H (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1454..1554
FT /note="SEQLDRLQKDLESTERQKELLSSTIKQQKQQFENCMDDLQGNELRLREHIHA
FT LKQAEEDVKNMASIIEKLKTQNKQKEKLIWEREMERNDSDMQLQETLLE -> P (in
FT Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1568
FT /note="D -> V (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1630..1646
FT /note="DLLKQLDHYTKVVEMLN -> SEAARSLYKSGGNVD (in Ref. 1;
FT CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1699..1705
FT /note="Missing (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1725..1737
FT /note="TLQLQMEQNSRNG -> NTTANGTKFKEW (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1754..1757
FT /note="FDDE -> LMM (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1777
FT /note="D -> E (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1788
FT /note="R -> T (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1825
FT /note="S -> D (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1882
FT /note="S -> W (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
FT CONFLICT 1902..1904
FT /note="FWK -> NSGKRLDADDL (in Ref. 1; CAA37894)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1928 AA; 223636 MW; 6F54C7611F43DC9F CRC64;
MTGGQSCSSN MIVWIPDEKE VFVKGELMST DINKNKFTGQ EEQIGIVHPL DSTEVSNLVQ
VRISDVFPVN PSTFDKVENM SELTHLNEPS VLYNLEKRYD CDLIYTYSGL FLVAINPYHN
LNLYSEDHIN LYHNKHNRLS KSRLDENSHE KLPPHIFAIA EEAYENLLSE GKDQSILVTG
ESGAGKTENT KKILQYLASI TSGSPSNIAP VSGSSIVESF EMKILQSNPI LESFGNAQTV
RNNNSSRFGK FIKIEFNEHG MINGAHIEWY LLEKSRIVHQ NSKERNYHIF YQLLSGLDDS
ELKNLRLKSR NVKDYKILSN SNQDIIPGIN DVENFKELLS ALNIIGFSKD QIRWIFQVVA
IILLIGNIEF VSDRAEQASF KNDVSAICSN LGVDEKDFQT AILRPRSKAG KEWVSQSKNS
QQAKFILNAL SRNLYERLFG YIVDMINKNL DHGSATLNYI GLLDIAGFEI FENNSFEQLC
INYTNEKLQQ FFNNHMFVLE QSEYLKENIQ WDYIDYGKDL QLTIDLIESK GPPTGVLPLL
DEEAVLPKST DESFYSKLIS TWDQNSSKFK RSRLKNGFIL KHYAGDVEYT VEGWLSKNKD
PLNDNLLSLL SSSQNDIISK LFQPEGEKSS SAGVEANISN QEVKKSARTS TFKTTSSRHR
EQQITLLNQL ASTHPHFVRC IIPNNVKKVK TFNRRLILDQ LRCNGVLEGI RLAREGYPNR
IAFQEFFQRY RILYPENSTT TTFSSKLKAS TKQNCEFLLT SLQLDTKVYK IGNTKLFFKA
GVLADLEKQK DVKLNNIMIK LTATIRGYTV RKEITYHLQK LKKTRVIGNT FRLYNRLVKE
DPWFNLFIRI KPLLTSSNDM TRTKKFNEQI NKLKNDLQEM ESKKKFLEEK NQKTVNELEN
TQDLLNQEKE NLRKNESLLN RVKTSSETLQ KQFDDLVSEK DEISREKLEV AQNLEEAHQK
IQGLQETIRE REATLEKLHS KNNELIKQIS DLNCDISKEQ SSQSLIKESK LKLENEIKRL
KDVINSKEEE IKSFNDKLSS SEEDLDIKLV TLEKNCNIAM SRLQSLVTEN SDLRSKNENF
KKEKAALNNQ LKNKESELLK MKEKIDNHKK ELATFSKQRD DAVSEHGKIT AELKETRIQL
TEYKSNYQKI KEEYSNFQRE TKEQEQKKRN SLVESLNDSK IKELEARLSQ EISLNQYLNK
RISGNSVETN ISSTRRSTSY SDDPLDKEDI IKKYYDLQLA FTEITRNLEN EIEEKKNLIS
RLRFTETRLA SSSFEDQKIK AQMKKLKKLI QDMDPSIPLD SILNEPLDNC PDKESDINKL
MLEVDYLKRQ LDIETRAHYD AENAISALHS KFRKIQGESS LSSSDIYKLK FEASEERVKS
LEDKLKTMPL RDRTNLPVGD IIKNRDSISK YEEEIRYYKL ENYKLQEILN ESNGKLSQLT
LDLRQSKSKE ALLSEQLDRL QKDLESTERQ KELLSSTIKQ QKQQFENCMD DLQGNELRLR
EHIHALKQAE EDVKNMASII EKLKTQNKQK EKLIWEREME RNDSDMQLQE TLLELKRVQD
VKKILSDDLA HLKERLSAVE DRSQYTDEIN RLKEELNCSL KAETNLKKEF ATLKYKLETS
TNDSEAKISD LLKQLDHYTK VVEMLNNEKD AISLAEKELY QKYEALNTEC ESLKGKIVSL
TKIKQELESD LNQKTDALQI SNAALSSSTQ KNKEITEKIK YLEETLQLQM EQNSRNGELV
KTLQASCNGY KDKFDDEKQK NIDLYEENQT LQKLNTDLQL QLKNLHERLS DTTEKNAWLS
KIHELENMVS LETDLKYEEM KKNKSLERAV EELQTKNSQQ TDVIELANKN RSEFEEATLK
YEAQISDLEK YISQQELEMK KSIRDNSSYR DKVQEMAQEI EFWKSRYEST MIGSKNIDSN
NAQSKIFS
