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MYO5B_MOUSE
ID   MYO5B_MOUSE             Reviewed;        1818 AA.
AC   P21271; Q148A3; Q69ZR6; Q811F6; Q91X59;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 146.
DE   RecName: Full=Unconventional myosin-Vb;
GN   Name=Myo5b; Synonyms=Kiaa1119;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=FVB/N; TISSUE=Colon, and Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1054-1818 (ISOFORM 3).
RC   TISSUE=Brain;
RX   PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA   Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA   Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA   Nagase T., Ohara O., Koga H.;
RT   "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT   The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT   identified by screening of terminal sequences of cDNA clones randomly
RT   sampled from size-fractionated libraries.";
RL   DNA Res. 11:205-218(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1086-1818 (ISOFORM 1).
RC   TISSUE=Brain;
RX   PubMed=2236059; DOI=10.1073/pnas.87.21.8491;
RA   Huang W.-M., Reed-Fourquet L., Wu E., Wu J.-Y.;
RT   "Molecular cloning and amino acid sequence of brain L-glutamate
RT   decarboxylase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:8491-8495(1990).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Kidney, Lung, Pancreas, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [5]
RP   INTERACTION WITH LIMA1.
RX   PubMed=29880681; DOI=10.1126/science.aao6575;
RA   Zhang Y.Y., Fu Z.Y., Wei J., Qi W., Baituola G., Luo J., Meng Y.J.,
RA   Guo S.Y., Yin H., Jiang S.Y., Li Y.F., Miao H.H., Liu Y., Wang Y., Li B.L.,
RA   Ma Y.T., Song B.L.;
RT   "A LIMA1 variant promotes low plasma LDL cholesterol and decreases
RT   intestinal cholesterol absorption.";
RL   Science 360:1087-1092(2018).
CC   -!- FUNCTION: May be involved in vesicular trafficking via its association
CC       with the CART complex. The CART complex is necessary for efficient
CC       transferrin receptor recycling but not for EGFR degradation. Required
CC       in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to
CC       the plasma membrane. Together with RAB11A participates in CFTR
CC       trafficking to the plasma membrane and TF (transferrin) recycling in
CC       nonpolarized cells. Together with RAB11A and RAB8A participates in
CC       epithelial cell polarization. Together with RAB25 regulates
CC       transcytosis. {ECO:0000250|UniProtKB:Q9ULV0}.
CC   -!- SUBUNIT: Component of the CART complex, at least composed of ACTN4,
CC       HGS/HRS, MYO5B and TRIM3. Interacts with RAB11FIP2 (By similarity).
CC       Interacts with RAB11A and RAB8A (By similarity). Found in a complex
CC       with CFTR and RAB11A (By similarity). Interacts with NPC1L1 (By
CC       similarity). Interacts with LIMA1 (PubMed:29880681). {ECO:0000250,
CC       ECO:0000269|PubMed:29880681}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P70569}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=P21271-1; Sequence=Displayed;
CC       Name=3;
CC         IsoId=P21271-3; Sequence=VSP_022099;
CC   -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC       superfamily. Myosin family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to be a glutamate decarboxylase (GAD).
CC       {ECO:0000305|PubMed:2236059}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA37655.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown origin in the N-terminal part.; Evidence={ECO:0000305};
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DR   EMBL; BC011494; AAH11494.1; -; mRNA.
DR   EMBL; BC046444; AAH46444.1; -; mRNA.
DR   EMBL; BC118525; AAI18526.1; -; mRNA.
DR   EMBL; AK173102; BAD32380.1; -; Transcribed_RNA.
DR   EMBL; M55253; AAA37655.1; ALT_SEQ; mRNA.
DR   CCDS; CCDS37858.1; -. [P21271-1]
DR   PIR; A36481; A36481.
DR   AlphaFoldDB; P21271; -.
DR   SMR; P21271; -.
DR   IntAct; P21271; 1.
DR   MINT; P21271; -.
DR   STRING; 10090.ENSMUSP00000073790; -.
DR   iPTMnet; P21271; -.
DR   PhosphoSitePlus; P21271; -.
DR   EPD; P21271; -.
DR   jPOST; P21271; -.
DR   MaxQB; P21271; -.
DR   PaxDb; P21271; -.
DR   PRIDE; P21271; -.
DR   ProteomicsDB; 287588; -. [P21271-1]
DR   ProteomicsDB; 287589; -. [P21271-3]
DR   MGI; MGI:106598; Myo5b.
DR   eggNOG; KOG0160; Eukaryota.
DR   InParanoid; P21271; -.
DR   PhylomeDB; P21271; -.
DR   Reactome; R-MMU-432040; Vasopressin regulates renal water homeostasis via Aquaporins.
DR   ChiTaRS; Myo5b; mouse.
DR   PRO; PR:P21271; -.
DR   Proteomes; UP000000589; Unplaced.
DR   RNAct; P21271; protein.
DR   GO; GO:0015629; C:actin cytoskeleton; IBA:GO_Central.
DR   GO; GO:0045179; C:apical cortex; ISS:UniProtKB.
DR   GO; GO:0005903; C:brush border; IDA:UniProtKB.
DR   GO; GO:0043197; C:dendritic spine; ISO:MGI.
DR   GO; GO:0098978; C:glutamatergic synapse; ISO:MGI.
DR   GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR   GO; GO:0043005; C:neuron projection; ISO:MGI.
DR   GO; GO:0043025; C:neuronal cell body; ISO:MGI.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR   GO; GO:0098871; C:postsynaptic actin cytoskeleton; ISO:MGI.
DR   GO; GO:0098944; C:postsynaptic recycling endosome membrane; ISO:MGI.
DR   GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR   GO; GO:0055037; C:recycling endosome; ISO:MGI.
DR   GO; GO:0098685; C:Schaffer collateral - CA1 synapse; IDA:SynGO.
DR   GO; GO:0051015; F:actin filament binding; ISO:MGI.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; ISO:MGI.
DR   GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR   GO; GO:0035255; F:ionotropic glutamate receptor binding; ISO:MGI.
DR   GO; GO:0000146; F:microfilament motor activity; IBA:GO_Central.
DR   GO; GO:0031267; F:small GTPase binding; ISO:MGI.
DR   GO; GO:0007015; P:actin filament organization; IBA:GO_Central.
DR   GO; GO:0060997; P:dendritic spine morphogenesis; ISO:MGI.
DR   GO; GO:0016197; P:endosomal transport; ISS:UniProtKB.
DR   GO; GO:0050804; P:modulation of chemical synaptic transmission; IDA:SynGO.
DR   GO; GO:0099639; P:neurotransmitter receptor transport, endosome to plasma membrane; ISO:MGI.
DR   GO; GO:0045773; P:positive regulation of axon extension; ISO:MGI.
DR   GO; GO:0050775; P:positive regulation of dendrite morphogenesis; ISO:MGI.
DR   GO; GO:1903543; P:positive regulation of exosomal secretion; ISO:MGI.
DR   GO; GO:1903078; P:positive regulation of protein localization to plasma membrane; ISO:MGI.
DR   GO; GO:0015031; P:protein transport; ISO:MGI.
DR   GO; GO:0099159; P:regulation of modification of postsynaptic structure; ISO:MGI.
DR   GO; GO:0032880; P:regulation of protein localization; ISO:MGI.
DR   GO; GO:0030050; P:vesicle transport along actin filament; IBA:GO_Central.
DR   GO; GO:0016192; P:vesicle-mediated transport; ISO:MGI.
DR   GO; GO:0099003; P:vesicle-mediated transport in synapse; ISO:MGI.
DR   CDD; cd15477; Myo5b_CBD; 1.
DR   CDD; cd01380; MYSc_Myo5; 1.
DR   Gene3D; 3.40.850.10; -; 1.
DR   InterPro; IPR002710; Dilute_dom.
DR   InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR   InterPro; IPR036961; Kinesin_motor_dom_sf.
DR   InterPro; IPR037990; Myo5b_CBD.
DR   InterPro; IPR001609; Myosin_head_motor_dom.
DR   InterPro; IPR004009; Myosin_N.
DR   InterPro; IPR036103; MYSc_Myo5.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01843; DIL; 1.
DR   Pfam; PF00612; IQ; 6.
DR   Pfam; PF00063; Myosin_head; 1.
DR   PRINTS; PR00193; MYOSINHEAVY.
DR   SMART; SM01132; DIL; 1.
DR   SMART; SM00015; IQ; 6.
DR   SMART; SM00242; MYSc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51126; DILUTE; 1.
DR   PROSITE; PS50096; IQ; 6.
DR   PROSITE; PS51456; MYOSIN_MOTOR; 1.
DR   PROSITE; PS51844; SH3_LIKE; 1.
PE   1: Evidence at protein level;
KW   Actin-binding; Alternative splicing; ATP-binding; Calmodulin-binding;
KW   Coiled coil; Cytoplasm; Motor protein; Myosin; Nucleotide-binding;
KW   Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport.
FT   CHAIN           1..1818
FT                   /note="Unconventional myosin-Vb"
FT                   /id="PRO_0000123461"
FT   DOMAIN          8..60
FT                   /note="Myosin N-terminal SH3-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01190"
FT   DOMAIN          69..762
FT                   /note="Myosin motor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00782"
FT   DOMAIN          765..794
FT                   /note="IQ 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT   DOMAIN          788..817
FT                   /note="IQ 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT   DOMAIN          813..842
FT                   /note="IQ 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT   DOMAIN          836..865
FT                   /note="IQ 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT   DOMAIN          861..890
FT                   /note="IQ 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT   DOMAIN          884..913
FT                   /note="IQ 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT   DOMAIN          1496..1773
FT                   /note="Dilute"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00503"
FT   REGION          21..40
FT                   /note="Requires for interaction with LIMA1"
FT                   /evidence="ECO:0000250|UniProtKB:Q9ULV0"
FT   REGION          641..663
FT                   /note="Actin-binding"
FT                   /evidence="ECO:0000255"
FT   REGION          1086..1120
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1161..1188
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          1140..1261
FT                   /evidence="ECO:0000255"
FT   COILED          1313..1415
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1095..1120
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         163..170
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         1416
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9ULV0"
FT   VAR_SEQ         1310
FT                   /note="N -> NSQTEDWGYLNEDGELGLAYQGLKQVA (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15368895"
FT                   /id="VSP_022099"
FT   CONFLICT        829
FT                   /note="A -> V (in Ref. 1; AAH46444)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        840
FT                   /note="T -> A (in Ref. 1; AAH46444)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        879..880
FT                   /note="KR -> RC (in Ref. 1; AAH46444)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        884
FT                   /note="E -> Q (in Ref. 1; AAH46444)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1217..1218
FT                   /note="KA -> NG (in Ref. 3; AAA37655)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1257
FT                   /note="V -> A (in Ref. 3; AAA37655)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1446
FT                   /note="D -> V (in Ref. 3; AAA37655)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1464
FT                   /note="S -> L (in Ref. 3; AAA37655)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1544
FT                   /note="T -> I (in Ref. 3; AAA37655)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1578..1579
FT                   /note="IA -> MP (in Ref. 3; AAA37655)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1620..1631
FT                   /note="Missing (in Ref. 3; AAA37655)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1818 AA;  210570 MW;  78CFFA626CADC4A0 CRC64;
     MSYSELYTRY TRVWIPDPDE VWRSAELTKD YKEGDKSLQL RLEDDTILEY PVDVQNNQVP
     FLRNPDILVG ENDLTALSHL HEPAVLHNLK VRFLESNHIY TYCGIVLVAI NPYEQLPIYG
     QDVIYAYSGQ NMGDMDPHIF AVAEEAYKQM ARDEKNQSII VSGESGAGKT VSAKYAMRYF
     ATVGGSASDT NIEEKVLASS PIMEAIGNAK TTRNDNSSRF GKFIEIGFDK KYHIIGANMR
     TYLLEKSRVV FQADDERNYH IFYQLCAAAS LPEFKELALT CAEDFFYTAH GGNTTIEGVN
     DADDFEKTRQ ALTLLGVRDS HQISIFKIIA SILHLGSVEI QSERDGDSCS ISPQDEHLSN
     FCSLLGIEHS QMEHWLCHRK LVTTSETYVK TMSLQQVVNA RDALAKHIYA QLFSWIVEHI
     NKALHTSHKQ HSFIGVLDIY GFETFEINSF EQFCINYANE KLQQQFNSHV FKLEQEEYMK
     EQIPWTLIDF YDNQPCIDLI EAKLGILDLL DEECKVPKGT DQNWAQKLYE RHSNSQHFQK
     PRMSNTAFIV NHFADKVEYL SDGFLEKNRD TVYEEQINIL KASKFPLVAD LFHDDKDSAP
     ATNTAKNRSS SKINVRSSRP LIKVPNKEHK KSVGYQFRTS LNLLMETLNA TTPHYVRCIK
     PNDEKLPFHF DPKRAVQQLR ACGVLETIRI SAAGYPSRWT YHDFFNRYRV LMKKRELTNT
     DKKNICKSVL ESLIKDPDKF QFGRTKIFFR AGQVAYLEKL RADKFREATI MIQKSVRGWL
     QRVKYRRLRA ATLSLQRFCR GYLARRLAEH LRRTRAAIVF QKQYRMLKAR RAYRRVCRAT
     VIIQSFTRAM FVRRNYRQVL MEHKATIIQK YARGWMARKR FLRERDAAIV IQCAFRRLKA
     RQELKALKIE ARSAEHLKRL NVGMENKVVQ LQRKIDDQNK EFKTLSEQLS AVTSSHAVEV
     EKLKKELAHY QQNQEADTSL QLQEEVQSLR TELQKAHSER RVLEDAHNKE NGELRKRVAD
     LEHENALLKD EKEYLNNQIL CQSKAESSQS SVEENLLMKK ELEEERSRYQ NLVKEYSQLE
     QRYENLRDEQ TPGHRKNPSN QSSLESDSNY PSISTSEIGD TEDALQQVEE IGIEKAAMDM
     TVFLKLQKRV RELEQERKKL QAQLEKGQQD SKKGQVEQQN NGLDVDQDAD IAYNSLKRQE
     LESENKKLKN DLNELRKAVA DQAMQDNSTH SSPDSYSLLL NQLKLANEEL EVRKEEVLIL
     RTQIMNADQR RLSGKNMEPN INARTSWPNS EKHVDQEDAI EAYHGVCQTN RLLEAQLQAQ
     SLEHEEEVEH LKAQVEALKE EMDKQQQTFC QTLLLSPEAQ VEFGVQQEIS RLTNENLDFK
     ELVEKLEKNE RKLKKQLKIY MKKVQDLEAA QALAQSDRRH HELTRQVTVQ RKEKDFQGML
     EYHKEDEALL IRNLVTDLKP QMLSGTVPCL PAYILYMCIR HADYTNDDLK VHSLLSSTIN
     GIKKVLKKHN DDFEMTSFWL SNTCRFLHCL KQYSGDEGFM TQNTAKQNEH CLKNFDLTEY
     RQVLSDLSIQ IYQQLIKIAE GLLQPMIVSA MLENESIQGL SGVRPTGYRK RSSSMVDGEN
     SYCLEAIVRQ MNSFHTVLCD QGLDPEIILQ VFKQLFYMIN AVTLNNLLLR KDACSWSTGM
     QLRYNISQLE EWLRGKNLHQ SGAVQTMEPL IQAAQLLQLK KKTHEDAEAI CSLCTSLSTQ
     QIVKILNLYT PLNEFEERVT VSFIRTIQAQ LQERNDPQQL LLDSKHVFPV LFPYNPSALT
     MDSIHIPACL NLEFLNEV
 
 
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