MYOA_DICDI
ID MYOA_DICDI Reviewed; 994 AA.
AC P22467; Q54VJ2;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Myosin IA heavy chain;
DE AltName: Full=Myosin-like protein abmA;
GN Name=myoA; Synonyms=abmA, dmiA; ORFNames=DDB_G0280039;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=A5-2;
RX PubMed=2519618; DOI=10.1091/mbc.1.1.55;
RA Titus M.A., Warrick H.M., Spudich J.A.;
RT "Multiple actin-based motor genes in Dictyostelium.";
RL Cell Regul. 1:55-63(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP NOMENCLATURE.
RX PubMed=16857047; DOI=10.1186/1471-2164-7-183;
RA Kollmar M.;
RT "Thirteen is enough: the myosins of Dictyostelium discoideum and their
RT light chains.";
RL BMC Genomics 7:183-183(2006).
CC -!- FUNCTION: Actin-based motor protein, possibly involved in a wide range
CC of motile processes, such as cell movement across a surface, and
CC extension and retraction of pseudopodia or lamellipodia.
CC -!- SUBUNIT: Myosin I heavy chain is single-headed. Dimer of a heavy and a
CC light chain. Inability to self-assemble into filaments.
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Myosin family. {ECO:0000305}.
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DR EMBL; S73909; AAB20711.1; -; Genomic_DNA.
DR EMBL; AAFI02000035; EAL67246.1; -; Genomic_DNA.
DR RefSeq; XP_641363.1; XM_636271.1.
DR AlphaFoldDB; P22467; -.
DR SMR; P22467; -.
DR STRING; 44689.DDB0215392; -.
DR PaxDb; P22467; -.
DR PRIDE; P22467; -.
DR EnsemblProtists; EAL67246; EAL67246; DDB_G0280039.
DR GeneID; 8622497; -.
DR KEGG; ddi:DDB_G0280039; -.
DR dictyBase; DDB_G0280039; myoA.
DR eggNOG; KOG0162; Eukaryota.
DR HOGENOM; CLU_000192_7_7_1; -.
DR InParanoid; P22467; -.
DR OMA; IKPNEYQ; -.
DR PhylomeDB; P22467; -.
DR PRO; PR:P22467; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0015629; C:actin cytoskeleton; IDA:dictyBase.
DR GO; GO:0031252; C:cell leading edge; IDA:dictyBase.
DR GO; GO:0005911; C:cell-cell junction; IDA:dictyBase.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR GO; GO:0031143; C:pseudopodium; IDA:dictyBase.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005516; F:calmodulin binding; IDA:dictyBase.
DR GO; GO:0000146; F:microfilament motor activity; IDA:dictyBase.
DR GO; GO:0030036; P:actin cytoskeleton organization; IGI:dictyBase.
DR GO; GO:0007015; P:actin filament organization; IBA:GO_Central.
DR GO; GO:0031152; P:aggregation involved in sorocarp development; IGI:dictyBase.
DR GO; GO:0048870; P:cell motility; IMP:dictyBase.
DR GO; GO:0043327; P:chemotaxis to cAMP; IMP:dictyBase.
DR GO; GO:0006897; P:endocytosis; IBA:GO_Central.
DR GO; GO:0016197; P:endosomal transport; IGI:dictyBase.
DR GO; GO:0120320; P:lateral pseudopodium retraction; IMP:dictyBase.
DR GO; GO:0006907; P:pinocytosis; IGI:dictyBase.
DR GO; GO:0051707; P:response to other organism; TAS:dictyBase.
DR GO; GO:0030050; P:vesicle transport along actin filament; IBA:GO_Central.
DR CDD; cd01378; MYSc_Myo1; 1.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR001609; Myosin_head_motor_dom.
DR InterPro; IPR010926; Myosin_TH1.
DR InterPro; IPR036072; MYSc_Myo1.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00612; IQ; 2.
DR Pfam; PF00063; Myosin_head; 1.
DR Pfam; PF06017; Myosin_TH1; 1.
DR PRINTS; PR00193; MYOSINHEAVY.
DR SMART; SM00015; IQ; 2.
DR SMART; SM00242; MYSc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50096; IQ; 1.
DR PROSITE; PS51456; MYOSIN_MOTOR; 1.
DR PROSITE; PS51757; TH1; 1.
PE 3: Inferred from homology;
KW Actin-binding; ATP-binding; Calmodulin-binding; Motor protein; Myosin;
KW Nucleotide-binding; Reference proteome; Repeat.
FT CHAIN 1..994
FT /note="Myosin IA heavy chain"
FT /id="PRO_0000123365"
FT DOMAIN 12..720
FT /note="Myosin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00782"
FT DOMAIN 723..744
FT /note="IQ 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT DOMAIN 745..774
FT /note="IQ 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT DOMAIN 782..970
FT /note="TH1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01093"
FT REGION 574..654
FT /note="Actin-binding"
FT BINDING 105..112
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 3
FT /note="T -> E (in Ref. 1; AAB20711)"
FT /evidence="ECO:0000305"
FT CONFLICT 152
FT /note="A -> G (in Ref. 1; AAB20711)"
FT /evidence="ECO:0000305"
FT CONFLICT 212..216
FT /note="SSEEE -> HQG (in Ref. 1; AAB20711)"
FT /evidence="ECO:0000305"
FT CONFLICT 239..242
FT /note="CFTA -> WFSLP (in Ref. 1; AAB20711)"
FT /evidence="ECO:0000305"
FT CONFLICT 270..271
FT /note="EI -> KS (in Ref. 1; AAB20711)"
FT /evidence="ECO:0000305"
FT CONFLICT 582
FT /note="R -> S (in Ref. 1; AAB20711)"
FT /evidence="ECO:0000305"
FT CONFLICT 637
FT /note="L -> S (in Ref. 1; AAB20711)"
FT /evidence="ECO:0000305"
FT CONFLICT 809..813
FT /note="ATLKR -> DALPS (in Ref. 1; AAB20711)"
FT /evidence="ECO:0000305"
FT CONFLICT 819..823
FT /note="AVLQL -> DCSSKR (in Ref. 1; AAB20711)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 994 AA; 113360 MW; EB6C5427516BF829 CRC64;
MATFKRDLTK NVGVEDLIML TEVSESSLHE NLKIRYKEGL IYTSIGPVLV SMNPYKQLGI
YGNDQINLYK GKHEFEIPPH IYSIADKAYR ALRSEGENQC IIISGESGAG KTEASKYIMQ
YIASITGSST EVERVKKTIL ESNPLLEAFG NAKTLRNNNS SRFGKYMEIQ FNLGGDPEGG
KITNYLLEKS RVINQTQGER NFHIFYQLLK GSSEEEKKTY NLLSPDQYHY LTRNASNGCF
TADGIDDQIG FKQTKNAMKV VGIDEPLQKE IFATLSAILL LGNLSFNKSA SGNGSVISDK
KLANTIASLM GVDAIVLESS LVSRQISTGQ GARISTYSVP QTVEQAMYAR DAFAKATYSK
LFDFIVRKIN QSIEVKTIGK VIGVLDIYGF EIFENNSFEQ FCINYVNETL QQIFIDLTLK
TEQEEYVQEG ITWIPVQYIN NKACVDLIEK KPIGILSLLD EECLFPEGND QTMIDKLNKH
FSNHTHYSKV ERQKNSQFII NHYAGKVFYN IDGFLDKNRD TLFNDLVTLA TSSSCSLLVE
IFKYVPPLEV DPEQEKKNRD KFSKNGFANN AAKTFIPTDK KRPITAGFQF KNQVTSLLKS
LYSCSPHYVR CIKPNSNMRA LEWDQSKCAE QVAYLGLFEN LLVRRAGYCY RQTFSKFMRR
YYMIGKSTWP KWSGDAEKGV NLLMGELTQE INIKEEVQYG KTKIFIRNPQ PLFLLEDKRN
KRLNDLATKI GSVWKMYKQR KWYLRTLAAI KIQRTYRGWL LVRECVKLKN QSISIFQNNK
ERNRQSIKLS KAAFIGDFLS LTRDNYTTAT LKREEGPNAV LQLSLNVSKV SRHHKIQKRS
LLVTNETIFT ITPHKPKKDG SWFAIKRKVP FSAIEKISFS KLSDDFFVIH IINEHDLCLE
TNKKTVLITL LSNLYSKHLN GKELVFEFKD SIQYRNQKGP SELKFVKVDS IHEKSSNSPQ
ANSPSFTAKA EKNYLKVCVL PGLSSESKSI LIEK
