MYOZ2_MOUSE
ID MYOZ2_MOUSE Reviewed; 264 AA.
AC Q9JJW5;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Myozenin-2;
DE AltName: Full=Calsarcin-1;
DE AltName: Full=FATZ-related protein 2;
GN Name=Myoz2 {ECO:0000312|MGI:MGI:1913063};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAG38939.1}
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=11114196; DOI=10.1073/pnas.260501097;
RA Frey N., Richardson J.A., Olson E.N.;
RT "Calsarcins, a novel family of sarcomeric calcineurin-binding proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:14632-14637(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:CAB92972.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Skeletal muscle {ECO:0000305};
RA Ievolella C., Zara I., Millino C., Faulkner G., Lanfranchi G.;
RT "Full length sequencing of some human and murine muscular transcripts
RT (Telethon Italy project B41).";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000312|EMBL:AAH24360.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II {ECO:0000312|EMBL:AAH24360.1};
RC TISSUE=Mammary gland {ECO:0000312|EMBL:AAH24360.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4] {ECO:0000305}
RP FUNCTION.
RX PubMed=15543153; DOI=10.1038/nm1132;
RA Frey N., Barrientos T., Shelton J.M., Frank D., Rutten H., Gehring D.,
RA Kuhn C., Lutz M., Rothermel B., Bassel-Duby R., Richardson J.A.,
RA Katus H.A., Hill J.A., Olson E.N.;
RT "Mice lacking calsarcin-1 are sensitized to calcineurin signaling and show
RT accelerated cardiomyopathy in response to pathological biomechanical
RT stress.";
RL Nat. Med. 10:1336-1343(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-111, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101; THR-107; THR-111 AND
RP SER-116, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-53, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- FUNCTION: Myozenins may serve as intracellular binding proteins
CC involved in linking Z line proteins such as alpha-actinin, gamma-
CC filamin, TCAP/telethonin, LDB3/ZASP and localizing calcineurin
CC signaling to the sarcomere. Plays an important role in the modulation
CC of calcineurin signaling. May play a role in myofibrillogenesis.
CC {ECO:0000269|PubMed:15543153}.
CC -!- SUBUNIT: Interacts via its C-terminus with spectrin repeats 3 and 4 of
CC ACTN2. Interacts with ACTN1, LDB3, MYOT and PPP3CA (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, myofibril, sarcomere, Z line
CC {ECO:0000269|PubMed:11114196}. Note=Colocalizes with ACTN1 and PPP3CA
CC at the Z-line of heart and skeletal muscle.
CC -!- TISSUE SPECIFICITY: Expressed specifically in heart and skeletal
CC muscle. In skeletal muscle, localized to the soleus and plantaris
CC muscles, which are predominantly composed of slow-twitch fibers.
CC {ECO:0000269|PubMed:11114196}.
CC -!- DEVELOPMENTAL STAGE: At 9.5 dpc, expressed weakly in heart. Higher
CC levels of expression detected at 12.5 dpc and 15.5 dpc in both cardiac
CC and skeletal muscle. {ECO:0000269|PubMed:11114196}.
CC -!- SIMILARITY: Belongs to the myozenin family. {ECO:0000305}.
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DR EMBL; AY013296; AAG38939.1; -; mRNA.
DR EMBL; AJ252148; CAB92972.1; -; mRNA.
DR EMBL; BC024360; AAH24360.1; -; mRNA.
DR CCDS; CCDS17816.1; -.
DR RefSeq; NP_067478.1; NM_021503.2.
DR AlphaFoldDB; Q9JJW5; -.
DR IntAct; Q9JJW5; 2.
DR STRING; 10090.ENSMUSP00000029761; -.
DR iPTMnet; Q9JJW5; -.
DR PhosphoSitePlus; Q9JJW5; -.
DR MaxQB; Q9JJW5; -.
DR PaxDb; Q9JJW5; -.
DR PRIDE; Q9JJW5; -.
DR ProteomicsDB; 287659; -.
DR Antibodypedia; 26655; 190 antibodies from 25 providers.
DR DNASU; 59006; -.
DR Ensembl; ENSMUST00000029761; ENSMUSP00000029761; ENSMUSG00000028116.
DR GeneID; 59006; -.
DR KEGG; mmu:59006; -.
DR UCSC; uc008rfe.2; mouse.
DR CTD; 51778; -.
DR MGI; MGI:1913063; Myoz2.
DR VEuPathDB; HostDB:ENSMUSG00000028116; -.
DR eggNOG; ENOG502QVA2; Eukaryota.
DR GeneTree; ENSGT00950000183027; -.
DR HOGENOM; CLU_071316_1_1_1; -.
DR InParanoid; Q9JJW5; -.
DR OMA; YTFENFH; -.
DR OrthoDB; 979261at2759; -.
DR PhylomeDB; Q9JJW5; -.
DR TreeFam; TF331748; -.
DR BioGRID-ORCS; 59006; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q9JJW5; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q9JJW5; protein.
DR Bgee; ENSMUSG00000028116; Expressed in interventricular septum and 88 other tissues.
DR ExpressionAtlas; Q9JJW5; baseline and differential.
DR Genevisible; Q9JJW5; MM.
DR GO; GO:0015629; C:actin cytoskeleton; IDA:MGI.
DR GO; GO:0030018; C:Z disc; IDA:BHF-UCL.
DR GO; GO:0003779; F:actin binding; IPI:MGI.
DR GO; GO:0051373; F:FATZ binding; IBA:GO_Central.
DR GO; GO:0031433; F:telethonin binding; ISO:MGI.
DR GO; GO:0070885; P:negative regulation of calcineurin-NFAT signaling cascade; IDA:BHF-UCL.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:BHF-UCL.
DR GO; GO:0045214; P:sarcomere organization; IDA:BHF-UCL.
DR GO; GO:0043503; P:skeletal muscle fiber adaptation; IDA:BHF-UCL.
DR GO; GO:0007519; P:skeletal muscle tissue development; IDA:BHF-UCL.
DR InterPro; IPR008438; MYOZ.
DR PANTHER; PTHR15941; PTHR15941; 1.
DR Pfam; PF05556; Calsarcin; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Methylation; Phosphoprotein; Reference proteome.
FT CHAIN 1..264
FT /note="Myozenin-2"
FT /id="PRO_0000111100"
FT REGION 90..135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 107..124
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 53
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 101
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 107
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 111
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 116
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 264 AA; 29762 MW; 2DCDC6A30B0704FD CRC64;
MLSHSAMVKQ RKQQASAITK EIHGHDVDGM DLGKKVSIPR DIMIEELSHF SNRGARLFKM
RQRRSDKYTF ENFQYESRAQ INHNIAMQNG RVDGSNLEGG SQQGPSTPPN TPDPRSPPNP
ENIAPGYSGP LKEIPPERFN TTAVPKYYRS PWEQAIGSDP ELLEALYPKL FKPEGKAELR
DYRSFNRVAT PFGGFEKASK MVKFKVPDFE LLLLTDPRFL AFANPLSGRR CFNRAPKGWV
SENIPVVITT EPTEDATVPE SDDL
