MYPN_MOUSE
ID MYPN_MOUSE Reviewed; 1315 AA.
AC Q5DTJ9; Q7TPW5; Q8BZ76;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Myopalladin;
GN Name=Mypn; Synonyms=Kiaa4170;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Fetal brain;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-754.
RC STRAIN=C57BL/6J; TISSUE=Bone;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1053-1315.
RC STRAIN=C57BL/6J; TISSUE=Blastocyst;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129 AND SER-924, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the sarcomere that tethers together nebulin
CC (skeletal muscle) and nebulette (cardiac muscle) to alpha-actinin, at
CC the Z lines. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with TTN/titin, NEB, NEBL, ACTN2 and CARP.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q86TC9}. Nucleus
CC {ECO:0000250|UniProtKB:Q86TC9}. Cytoplasm, myofibril, sarcomere
CC {ECO:0000250|UniProtKB:Q86TC9}. Cytoplasm, myofibril, sarcomere, Z line
CC {ECO:0000250|UniProtKB:Q86TC9}. Note=Bound to sarcomere both at the Z-
CC line periphery and in the central I-band region.
CC {ECO:0000250|UniProtKB:Q86TC9}.
CC -!- SIMILARITY: Belongs to the myotilin/palladin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD90521.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK220521; BAD90521.1; ALT_INIT; mRNA.
DR EMBL; AK036458; BAC29439.1; -; mRNA.
DR EMBL; BC052872; AAH52872.1; -; mRNA.
DR CCDS; CCDS48584.1; -.
DR RefSeq; NP_892037.2; NM_182992.2.
DR AlphaFoldDB; Q5DTJ9; -.
DR SMR; Q5DTJ9; -.
DR BioGRID; 213060; 8.
DR IntAct; Q5DTJ9; 1.
DR STRING; 10090.ENSMUSP00000093240; -.
DR iPTMnet; Q5DTJ9; -.
DR PhosphoSitePlus; Q5DTJ9; -.
DR MaxQB; Q5DTJ9; -.
DR PaxDb; Q5DTJ9; -.
DR PeptideAtlas; Q5DTJ9; -.
DR PRIDE; Q5DTJ9; -.
DR ProteomicsDB; 287599; -.
DR Antibodypedia; 28426; 116 antibodies from 21 providers.
DR DNASU; 68802; -.
DR Ensembl; ENSMUST00000095580; ENSMUSP00000093240; ENSMUSG00000020067.
DR GeneID; 68802; -.
DR KEGG; mmu:68802; -.
DR UCSC; uc007fjy.2; mouse.
DR CTD; 84665; -.
DR MGI; MGI:1916052; Mypn.
DR VEuPathDB; HostDB:ENSMUSG00000020067; -.
DR eggNOG; ENOG502QSRV; Eukaryota.
DR GeneTree; ENSGT00940000153441; -.
DR HOGENOM; CLU_006487_2_0_1; -.
DR InParanoid; Q5DTJ9; -.
DR OMA; RSHQQEY; -.
DR OrthoDB; 100208at2759; -.
DR PhylomeDB; Q5DTJ9; -.
DR TreeFam; TF343193; -.
DR BioGRID-ORCS; 68802; 5 hits in 72 CRISPR screens.
DR ChiTaRS; Mypn; mouse.
DR PRO; PR:Q5DTJ9; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q5DTJ9; protein.
DR Bgee; ENSMUSG00000020067; Expressed in gastrocnemius medialis and 89 other tissues.
DR ExpressionAtlas; Q5DTJ9; baseline and differential.
DR Genevisible; Q5DTJ9; MM.
DR GO; GO:0030424; C:axon; IBA:GO_Central.
DR GO; GO:0031674; C:I band; IDA:BHF-UCL.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0030018; C:Z disc; ISO:MGI.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR GO; GO:0098632; F:cell-cell adhesion mediator activity; IBA:GO_Central.
DR GO; GO:0008092; F:cytoskeletal protein binding; ISO:MGI.
DR GO; GO:0051371; F:muscle alpha-actinin binding; ISO:MGI.
DR GO; GO:0017124; F:SH3 domain binding; ISO:MGI.
DR GO; GO:0007411; P:axon guidance; IBA:GO_Central.
DR GO; GO:0070593; P:dendrite self-avoidance; IBA:GO_Central.
DR GO; GO:0007156; P:homophilic cell adhesion via plasma membrane adhesion molecules; IBA:GO_Central.
DR GO; GO:0045214; P:sarcomere organization; ISO:MGI.
DR Gene3D; 2.60.40.10; -; 5.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR013098; Ig_I-set.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR003598; Ig_sub2.
DR Pfam; PF07679; I-set; 5.
DR SMART; SM00409; IG; 5.
DR SMART; SM00408; IGc2; 5.
DR SUPFAM; SSF48726; SSF48726; 5.
DR PROSITE; PS50835; IG_LIKE; 5.
PE 1: Evidence at protein level;
KW Actin-binding; Coiled coil; Cytoplasm; Disulfide bond;
KW Immunoglobulin domain; Nucleus; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..1315
FT /note="Myopalladin"
FT /id="PRO_0000240490"
FT DOMAIN 267..357
FT /note="Ig-like 1"
FT DOMAIN 432..528
FT /note="Ig-like 2"
FT DOMAIN 941..1025
FT /note="Ig-like 3"
FT DOMAIN 1068..1157
FT /note="Ig-like 4"
FT DOMAIN 1167..1257
FT /note="Ig-like 5"
FT REGION 19..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 81..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 166..204
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 230..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 359..392
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 535..652
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 674..704
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 725..747
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 762..814
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 840..865
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 219..240
FT /evidence="ECO:0000255"
FT COMPBIAS 20..36
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 83..103
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 122..145
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 538..562
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 610..627
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 795..814
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 99
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86TC9"
FT MOD_RES 129
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 249
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q86TC9"
FT MOD_RES 641
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86TC9"
FT MOD_RES 754
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86TC9"
FT MOD_RES 809
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86TC9"
FT MOD_RES 814
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86TC9"
FT MOD_RES 903
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q86TC9"
FT MOD_RES 924
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT DISULFID 288..339
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 453..512
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT DISULFID 1089..1141
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT CONFLICT 1120
FT /note="E -> G (in Ref. 3; AAH52872)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1315 AA; 144114 MW; 3CBA735F63983B3F CRC64;
MQEDSIEAST SISQLLRESY LAETRHRGDN ERSRAEPSSN PFHFSGPGAA EGGGPEDLPD
LSAFLSQEEL DESVNLARLA INHDPLERVD EAQARKRLSS DQTKHASKPS FEPAFHQDSS
RGPASPKDSP PETKRPQYSS ETQSKKVFLN KAADFIEELS SLFKAHSSKR IRPRACKNHK
SKTESQNKVL QENSPTFSDL TERRERASVP IPIPADSRDN ELNHAIEQRE AKRREAELAA
GEAAAGDSTP GSSPSSLYYE EPLGQPPRFT QKLRSREVPE GSRVQLDCIV VGIPPPQVRW
YCEGKELENS PDIHIVQAGN LHSLTIAEAF EEDTGRYSCF ASNIYGTDST SAEIYIEGVS
SSDSEGDPNK EEMNRIQKPN EVSSPPTTSA AIPPAAEAQP LAAQPRVSTV QQCQSPTNYL
QGLNGKPIIA APVFTKMLQN LSASEGQLVV FECRVKGAPS PKVEWYREGT LIEDSPDFRI
LQKKPRSMAE PEEICTLVIA EVFSEDSGCF TCTASNKYGT VSSIAQLDVR GNEDISDNGA
LHSANSTTNP AVAEHQPSPL NPQPLSEEQP PKPKLEGVLV NHNEPRSSSR IGLRVHFNLP
EDDKDMEASS GSGAANTSQT RPNSFPERFN GQEARIPEPS SPIKEPPPVL AKPKLDSTQL
QQLHNQVLLE QQQLQNTSPS SPKESLHMSA LNSAPPAVTI SSKQVKGPAP QMFNLARPKH
FFPASSTSTA TVSPSSSPVF TLSNTPQTIQ RTVSKESLLM AHPSTQGRSP GGLSIQNEPA
PPSPAEPAAP PTAAYSIPSG NQFQPHCVSP TPVSPTGRIQ NPVAFLSSVL PSLPSIPPTN
AMGLPKSAPS VPSQGLMKKT TKAPQAVSDD YIRETKNSVI LDLGKKVNFG DVRSHQQEYK
ISSFEQRLMN EIEFRLERTP VDESDDEIEH DEIPTGKCIA PIFDKRLKHF RVTEGSPVTF
TCKIVGIPVP KVYWFKDGKQ ISKRNEHCKM RREGDGTCSL HIESTHGDDD GNYTIMAANP
QGRISCSGHL MVQGLPIRSR LSPALSHRGR SRMQERDKEP LQERFFRPHF LQAPGDMVAH
EGRLCRLDCK VSGLPPPELT WLLNGQPVLP DASHKMLVRE TGVHSLLIDP LTQRDAGTYT
CVATNKTGQN SFSLELTVVA KEVKKAPVIL EKLQNSGVPE GHPVRLEGRV IGMPPPVFYW
KKDNETIPFT RERISMHQDT TGYVCLLIQP AKKSDAGWYT LSAKNEAGIV SCTARLDIYA
QWHQQIPTPI SIRPSGSRYG SLTSKGLDIF SAFSSVESTM LYSCTSRSVV ESDEL
