ID   MYRO2_BREBR             Reviewed;         140 AA.
AC   P83179;
DT   17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 2.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=Myrosinase 2;
DE            EC=3.2.1.147;
DE   AltName: Full=Beta-glucosidase 2;
DE   AltName: Full=Beta-thioglucosidase 2;
DE   AltName: Full=Beta-thioglucosidase glucohydrolase 2;
DE   AltName: Full=Myrosinase;
DE   AltName: Full=Sinigrinase 2;
DE   AltName: Full=Thioglucosidase 2;
DE   Flags: Fragments;
OS   Brevicoryne brassicae (Mealy cabbage aphid).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aphidomorpha;
OC   Aphidoidea; Aphididae; Macrosiphini; Brevicoryne.
OX   NCBI_TaxID=69196 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION,
RP   BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RX   PubMed=11179970; DOI=10.1046/j.1432-1327.2001.01971.x;
RA   Pontoppidan B., Ekbom B., Eriksson S., Meijer J.;
RT   "Purification and characterization of myrosinase from the cabbage aphid
RT   (Brevicoryne brassicae), a brassica herbivore.";
RL   Eur. J. Biochem. 268:1041-1048(2001).
CC   -!- FUNCTION: Degradation of glucosinolates (glucose residue linked by a
CC       thioglucoside bound to an amino acid derivative) to glucose, sulfate
CC       and any of the products: thiocyanates, isothiocyanates, nitriles,
CC       epithionitriles or oxazolidine-2-thiones.
CC       {ECO:0000250|UniProtKB:P37702, ECO:0000269|PubMed:11179970}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a thioglucoside + H2O = a sugar + a thiol.; EC=3.2.1.147;
CC         Evidence={ECO:0000269|PubMed:11179970};
CC   -!- ACTIVITY REGULATION: Inhibited by ascorbate.
CC       {ECO:0000269|PubMed:11179970}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.41 mM for sinigrin (at pH 4.5 and 37 degrees Celsius)
CC         {ECO:0000269|PubMed:11179970};
CC         KM=0.52 mM for p-nitrophenyl-beta-glucopyranoside (at pH 4.5 and 37
CC         degrees Celsius) {ECO:0000269|PubMed:11179970};
CC       Temperature dependence:
CC         Optimum temperature is about 40 degrees Celsius.
CC         {ECO:0000269|PubMed:11179970};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:11179970}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. {ECO:0000305}.
CC   -!- CAUTION: The order of the peptides shown is unknown. {ECO:0000305}.
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DR   AlphaFoldDB; P83179; -.
DR   SMR; P83179; -.
DR   GO; GO:0102799; F:glucosinolate glucohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019137; F:thioglucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   SUPFAM; SSF51445; SSF51445; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase.
FT   CHAIN           <1..>140
FT                   /note="Myrosinase 2"
FT                   /id="PRO_0000063904"
FT   ACT_SITE        70
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10055"
FT   CARBOHYD        114
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        127
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   NON_CONS        12..13
FT                   /evidence="ECO:0000303|PubMed:11179970"
FT   NON_CONS        26..27
FT                   /evidence="ECO:0000303|PubMed:11179970"
FT   NON_CONS        35..36
FT                   /evidence="ECO:0000303|PubMed:11179970"
FT   NON_CONS        44..45
FT                   /evidence="ECO:0000303|PubMed:11179970"
FT   NON_CONS        58..59
FT                   /evidence="ECO:0000303|PubMed:11179970"
FT   NON_CONS        84..85
FT                   /evidence="ECO:0000303|PubMed:11179970"
FT   NON_CONS        97..98
FT                   /evidence="ECO:0000303|PubMed:11179970"
FT   NON_CONS        111..112
FT                   /evidence="ECO:0000303|PubMed:11179970"
FT   NON_CONS        124..125
FT                   /evidence="ECO:0000303|PubMed:11179970"
FT   NON_TER         1
FT                   /evidence="ECO:0000303|PubMed:11179970"
FT   NON_TER         140
FT                   /evidence="ECO:0000303|PubMed:11179970"
SQ   SEQUENCE   140 AA;  15646 MW;  A03EF5F737CD8ABD CRC64;
     DFMFGTSTAS GGLAPSGVMN SLEPKGLAYY NNLLNELLKV LFTYFGDRAY APNSPQRLSL
     SLSGVFHLLR GTADFYALNH YSSRDKYGLP KLLLTEDGYG DDGQLDDFEK NYLNATLQAM
     YLMKEQNVTS VHYTVNKCMN