MYSP1_DROME
ID MYSP1_DROME Reviewed; 879 AA.
AC P35415; Q8T0Q3; Q9VSP6;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Paramyosin, long form;
GN Name=Prm; ORFNames=CG5939;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Canton-S; TISSUE=Muscle;
RX PubMed=1730773; DOI=10.1083/jcb.116.3.669;
RA Becker K.D., O'Donnell P.T., Heitz J.M., Vito M., Bernstein S.I.;
RT "Analysis of Drosophila paramyosin: identification of a novel isoform which
RT is restricted to a subset of adult muscles.";
RL J. Cell Biol. 116:669-681(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1371577; DOI=10.1007/bf00292707;
RA Vinos J., Maroto M., Garesse R., Marco R., Cervera M.;
RT "Drosophila melanogaster paramyosin: developmental pattern, mapping and
RT properties deduced from its complete coding sequence.";
RL Mol. Gen. Genet. 231:385-394(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 103-571, AND CHARACTERIZATION.
RC STRAIN=Oregon-R;
RX PubMed=1908014; DOI=10.1016/0022-2836(91)90110-r;
RA Vinos J., Domingo A., Marco R., Cervera M.;
RT "Identification and characterization of Drosophila melanogaster
RT paramyosin.";
RL J. Mol. Biol. 220:687-700(1991).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Paramyosin is a major structural component of many thick
CC filaments isolated from invertebrate muscles.
CC -!- SUBUNIT: Heterodimer of two isoforms. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, myofibril. Note=Thick filaments of the
CC myofibrils.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms seem to exist. Isoforms can also be
CC produced by post-translational modifications.;
CC Name=Long; Synonyms=A, B;
CC IsoId=P35415-1; Sequence=Displayed;
CC Name=Short; Synonyms=C, D;
CC IsoId=P35416-1; Sequence=External;
CC -!- TISSUE SPECIFICITY: Expressed in all larval and adult muscle tissues.
CC Expression is five times higher in tubular than in fibrillar muscles.
CC -!- DEVELOPMENTAL STAGE: Undetectable during gastrulation and early phases
CC of germ band formation. Increases during organogenesis, around 10 hours
CC post-fertilization (hpf), to the adult stage.
CC -!- PTM: The more-acidic and less-abundant isoform is phosphorylated.
CC {ECO:0000269|PubMed:18327897}.
CC -!- SIMILARITY: Belongs to the paramyosin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL39277.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; X62590; CAA44475.1; -; mRNA.
DR EMBL; X58722; CAA41557.1; -; mRNA.
DR EMBL; AE014296; AAF50370.1; -; Genomic_DNA.
DR EMBL; AY069132; AAL39277.1; ALT_SEQ; mRNA.
DR PIR; S22028; S22028.
DR RefSeq; NP_523982.2; NM_079258.3. [P35415-1]
DR RefSeq; NP_729405.1; NM_168290.3. [P35415-1]
DR AlphaFoldDB; P35415; -.
DR SMR; P35415; -.
DR BioGRID; 64408; 60.
DR DIP; DIP-17473N; -.
DR IntAct; P35415; 9.
DR STRING; 7227.FBpp0076320; -.
DR iPTMnet; P35415; -.
DR PaxDb; P35415; -.
DR PRIDE; P35415; -.
DR DNASU; 39002; -.
DR EnsemblMetazoa; FBtr0076593; FBpp0076320; FBgn0003149. [P35415-1]
DR EnsemblMetazoa; FBtr0076594; FBpp0076321; FBgn0003149. [P35415-1]
DR GeneID; 39002; -.
DR KEGG; dme:Dmel_CG5939; -.
DR CTD; 39002; -.
DR FlyBase; FBgn0003149; Prm.
DR VEuPathDB; VectorBase:FBgn0003149; -.
DR eggNOG; KOG0161; Eukaryota.
DR GeneTree; ENSGT00940000173651; -.
DR HOGENOM; CLU_000192_13_6_1; -.
DR InParanoid; P35415; -.
DR OMA; FELPYSH; -.
DR PhylomeDB; P35415; -.
DR SignaLink; P35415; -.
DR BioGRID-ORCS; 39002; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Prm; fly.
DR GenomeRNAi; 39002; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0003149; Expressed in oviduct (Drosophila) and 24 other tissues.
DR ExpressionAtlas; P35415; baseline and differential.
DR Genevisible; P35415; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005856; C:cytoskeleton; IBA:GO_Central.
DR GO; GO:0031430; C:M band; HDA:FlyBase.
DR GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR GO; GO:0005863; C:striated muscle myosin thick filament; HDA:FlyBase.
DR GO; GO:0031032; P:actomyosin structure organization; IBA:GO_Central.
DR GO; GO:0007498; P:mesoderm development; IEP:FlyBase.
DR GO; GO:0030239; P:myofibril assembly; IMP:FlyBase.
DR Gene3D; 1.20.5.370; -; 1.
DR InterPro; IPR002928; Myosin_tail.
DR InterPro; IPR014751; XRCC4-like_C.
DR Pfam; PF01576; Myosin_tail_1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Disulfide bond;
KW Motor protein; Muscle protein; Myosin; Phosphoprotein; Reference proteome;
KW Thick filament.
FT CHAIN 1..879
FT /note="Paramyosin, long form"
FT /id="PRO_0000211249"
FT REGION 1..31
FT /note="Nonhelical region"
FT /evidence="ECO:0000255"
FT REGION 859..879
FT /note="Nonhelical region"
FT /evidence="ECO:0000255"
FT COILED 32..858
FT /evidence="ECO:0000255"
FT MOD_RES 18
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT DISULFID 368
FT /note="Interchain"
FT /evidence="ECO:0000255"
FT DISULFID 784
FT /note="Interchain"
FT /evidence="ECO:0000255"
FT CONFLICT 500
FT /note="Missing (in Ref. 2; CAA41557)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 879 AA; 102338 MW; 85BB333519815A1A CRC64;
MSSSQAVRSS KYSYRATSTG PGTADVNIEY IQDLSSLSRL EDKIRLLQDD LEVERELRQR
IEREKADLSV QVIQMSERLE EAEGGAEHQF EANRKRDAEL LKLRKLLEDV HLESEETTLL
LKKKHNEIIT DFQEQVEILT KNKARAEKDK AKFQTEVYEL LSQIESYNKE KIVSEKHISK
LEVSISELNV KIEELNRTVI DISSHRSRLS QENIELTKDV QDLKVQLDTV SFSKSQVISQ
LEDARRRLED EDRRRSLLES SLHQVEIELD SVRNQLEEES EARIDLERQL VKANADATSW
QNKWNSEVAA RAEEVEEIRR KYQVRITELE EHIESLIVKV NNLEKMKTRL ASEVEVLIID
LEKSNNSCRE LTKSVNTLEK HNVELKSRLD ETIILYETSQ RDLKNKHADL VRTVHELDKV
KDNNNQLTRE NKKLGDDLHE AKGAINELNR RLHELELELR RLENERDELT AAYKEAEAGR
KAEEQRGQRL AADFNQYRHD AERRLAEKDE EIEAIRKQTS IEIEQLNARV IEAETRLKTE
VTRIKKKLQI QITELEMSLD VANKTNIDLQ KVIKKQSLQL TELQAHYEDV QRQLQATLDQ
YAVAQRRLAG LNGELEEVRS HLDSANRAKR TVELQYEEAA SRINELTTAN VSLVSIKSKL
EQELSVVASD YEEVSKELRI SDERYQKVQV ELKHVVEQVH EEQERIVKLE TIKKSLEVEV
KNLSIRLEEV ELNAVAGSKR IISKLEARIR DLELELEEEK RRHAETIKIL RKKERTVKEV
LVQCEEDQKN LILLQDALDK STAKINIYRR QLSEQEGVSQ QTTTRVRRFQ RELEAAEDRA
DTAESSLNII RAKHRTFVTT STVPGSQVYI QETTRTITE
