MYSP2_DROME
ID MYSP2_DROME Reviewed; 640 AA.
AC P35416; Q961L6; Q9VSP5;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Paramyosin, short form;
DE AltName: Full=Miniparamyosin;
GN Name=Prm; ORFNames=CG5939;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-276.
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 118-640.
RC STRAIN=Canton-S; TISSUE=Muscle;
RX PubMed=1730773; DOI=10.1083/jcb.116.3.669;
RA Becker K.D., O'Donnell P.T., Heitz J.M., Vito M., Bernstein S.I.;
RT "Analysis of Drosophila paramyosin: identification of a novel isoform which
RT is restricted to a subset of adult muscles.";
RL J. Cell Biol. 116:669-681(1992).
CC -!- FUNCTION: Paramyosin is a major structural component of many thick
CC filaments isolated from invertebrate muscles.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, myofibril. Note=Thick filaments of the
CC myofibrils.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms seem to exist. Isoforms can also be
CC produced by post-translational modifications.;
CC Name=Short; Synonyms=C, D;
CC IsoId=P35416-1; Sequence=Displayed;
CC Name=Long; Synonyms=A, B;
CC IsoId=P35415-1; Sequence=External;
CC -!- TISSUE SPECIFICITY: Found in all adult muscle tissues except in
CC indirect flight muscles and a set of temporary abdominal muscles. Not
CC detected in larval muscle.
CC -!- PTM: Phosphorylated.
CC -!- SIMILARITY: Belongs to the paramyosin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA44476.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014296; AAF50371.2; -; Genomic_DNA.
DR EMBL; AY051521; AAK92945.1; ALT_SEQ; mRNA.
DR EMBL; X62591; CAA44476.1; ALT_FRAME; mRNA.
DR PIR; S22027; S22027.
DR RefSeq; NP_729406.1; NM_168291.1. [P35416-1]
DR RefSeq; NP_729407.1; NM_168292.2. [P35416-1]
DR AlphaFoldDB; P35416; -.
DR SMR; P35416; -.
DR BioGRID; 64408; 60.
DR PRIDE; P35416; -.
DR DNASU; 39002; -.
DR EnsemblMetazoa; FBtr0076595; FBpp0076322; FBgn0003149. [P35416-1]
DR EnsemblMetazoa; FBtr0076596; FBpp0076323; FBgn0003149. [P35416-1]
DR GeneID; 39002; -.
DR CTD; 39002; -.
DR FlyBase; FBgn0003149; Prm.
DR VEuPathDB; VectorBase:FBgn0003149; -.
DR GeneTree; ENSGT00940000173651; -.
DR HOGENOM; CLU_027023_0_0_1; -.
DR BioGRID-ORCS; 39002; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Prm; fly.
DR GenomeRNAi; 39002; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0003149; Expressed in oviduct (Drosophila) and 24 other tissues.
DR ExpressionAtlas; P35416; baseline and differential.
DR Genevisible; P35416; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005856; C:cytoskeleton; IBA:GO_Central.
DR GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR GO; GO:0005863; C:striated muscle myosin thick filament; HDA:FlyBase.
DR GO; GO:0031032; P:actomyosin structure organization; IBA:GO_Central.
DR GO; GO:0007498; P:mesoderm development; IEP:FlyBase.
DR GO; GO:0030239; P:myofibril assembly; IMP:FlyBase.
DR Gene3D; 1.20.5.370; -; 1.
DR InterPro; IPR002928; Myosin_tail.
DR InterPro; IPR014751; XRCC4-like_C.
DR Pfam; PF01576; Myosin_tail_1; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Cytoplasm; Motor protein;
KW Muscle protein; Myosin; Phosphoprotein; Reference proteome; Thick filament.
FT CHAIN 1..640
FT /note="Paramyosin, short form"
FT /id="PRO_0000211250"
FT REGION 1..122
FT /note="Nonhelical region"
FT /evidence="ECO:0000255"
FT REGION 420..640
FT /note="Nonhelical region"
FT /evidence="ECO:0000255"
FT COILED 123..619
FT /evidence="ECO:0000255"
FT CONFLICT 118
FT /note="E -> Q (in Ref. 4)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 640 AA; 74277 MW; 020966960AB68D61 CRC64;
MALALKQRPS RFRPTTTTYE DNYGYTMNFY QPMLDYLDAK AKGLEVKKPH LPWVSERGLK
QYRPSNAVRQ YNADEIVRLS RTCAARADEI LLNFRAQKRS PFSVQKLVDA SRVTKHLEPD
TVVERSRQRR RRRQEELEDL IKRDTLKILQ RIRKIELDNE LDKMSDDFKR SIRGKSASAI
AQALLSESEK NIKTAKKEEE DYIAQTLVRS SRAVSRARSR SSSPLDGQYR AHALHIELMD
DRLVDKLDHR VSSSLHNVKR QLSTLNQRTV EFYADSSKQT SIEIEQLNAR VIEAETRLKT
EVTRIKKKLQ IQITELEMSL DVANKTNIDL QKVIKKQSLQ LTELQAHYED VQRQLQATLD
QYAVAQRRLA GLNGELEEVR SHLDSANRAK RTVELQYEEA ASRINELTTA NVSLVSIKSK
LEQELSVVAS DYEEVSKELR ISDERYQKVQ VELKHVVEQV HEEQERIVKL ETIKKSLEVE
VKNLSIRLEE VELNAVAGSK RIISKLEARI RDLELELEEE KRRHAETIKI LRKKERTVKE
VLVQCEEDQK NLILLQDALD KSTAKINIYR RQLSEQEGVS QQTTTRVRRF QRELEAAEDR
ADTAESSLNI IRAKHRTFVT TSTVPGSQVY IQETTRTITE
