MYTR_MITCE
ID MYTR_MITCE Reviewed; 198 AA.
AC P39047;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Mitrocomin;
DE Flags: Precursor;
GN Name=MI17;
OS Mitrocoma cellularia (Cross jellyfish) (Earleria cellularia).
OC Eukaryota; Metazoa; Cnidaria; Hydrozoa; Hydroidolina; Leptothecata;
OC Mitrocomidae; Mitrocoma.
OX NCBI_TaxID=31874;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8224198; DOI=10.1016/0014-5793(93)80675-k;
RA Fagan T.F., Ohmiya Y., Blinks J.R., Inouye S., Tsuji F.I.;
RT "Cloning, expression and sequence analysis of cDNA for the Ca(2+)-binding
RT photoprotein, mitrocomin.";
RL FEBS Lett. 333:301-305(1993).
CC -!- FUNCTION: Ca(2+)-dependent bioluminescence photoprotein. Displays an
CC emission peak at 470 nm (blue light). Trace amounts of calcium ion
CC trigger the intramolecular oxidation of the chromophore, coelenterazine
CC into coelenteramide and CO(2) with the concomitant emission of light.
CC -!- SIMILARITY: Belongs to the aequorin family. {ECO:0000305}.
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DR EMBL; L31623; AAA29298.1; -; mRNA.
DR PIR; S39022; S39022.
DR PDB; 4NQG; X-ray; 1.30 A; A/B=2-198.
DR PDBsum; 4NQG; -.
DR AlphaFoldDB; P39047; -.
DR SMR; P39047; -.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13202; EF-hand_5; 2.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 3.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Luminescence; Metal-binding; Photoprotein; Repeat.
FT PROPEP 1..8
FT /evidence="ECO:0000255"
FT /id="PRO_0000004132"
FT CHAIN 9..198
FT /note="Mitrocomin"
FT /id="PRO_0000004133"
FT DOMAIN 19..54
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 118..147
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 148..183
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 32
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 34
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 36
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 38
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 43
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 125
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 127
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 129
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 131
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 136
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 161
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 163
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 165
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 167
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 172
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT HELIX 18..31
FT /evidence="ECO:0007829|PDB:4NQG"
FT STRAND 36..39
FT /evidence="ECO:0007829|PDB:4NQG"
FT HELIX 41..50
FT /evidence="ECO:0007829|PDB:4NQG"
FT HELIX 52..55
FT /evidence="ECO:0007829|PDB:4NQG"
FT HELIX 60..76
FT /evidence="ECO:0007829|PDB:4NQG"
FT HELIX 87..106
FT /evidence="ECO:0007829|PDB:4NQG"
FT HELIX 112..124
FT /evidence="ECO:0007829|PDB:4NQG"
FT STRAND 129..132
FT /evidence="ECO:0007829|PDB:4NQG"
FT HELIX 134..144
FT /evidence="ECO:0007829|PDB:4NQG"
FT HELIX 150..159
FT /evidence="ECO:0007829|PDB:4NQG"
FT HELIX 170..181
FT /evidence="ECO:0007829|PDB:4NQG"
FT HELIX 186..188
FT /evidence="ECO:0007829|PDB:4NQG"
FT TURN 189..194
FT /evidence="ECO:0007829|PDB:4NQG"
SQ SEQUENCE 198 AA; 22714 MW; 8F6307EF0966F670 CRC64;
MSMGSRYAVK LTTDFDNPKW IARHKHMFNF LDINSNGQIN LNEMVHKASN IICKKLGATE
EQTKRHQKCV EDFFGGAGLE YDKDTTWPEY IEGWKRLAKT ELERHSKNQV TLIRLWGDAL
FDIIDKDRNG SVSLDEWIQY THCAGIQQSR GQCEATFAHC DLDGDGKLDV DEMTRQHLGF
WYSVDPTCEG LYGGAVPY
