MYX1_CROVV
ID MYX1_CROVV Reviewed; 42 AA.
AC P01476;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Myotoxin-A;
DE AltName: Full=Myotoxin-1;
OS Crotalus viridis viridis (Prairie rattlesnake).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Viperidae; Crotalinae; Crotalus.
OX NCBI_TaxID=8742;
RN [1]
RP PROTEIN SEQUENCE, DISULFIDE BONDS, AND SUBCELLULAR LOCATION.
RX PubMed=570412; DOI=10.1021/bi00571a020;
RA Fox J.W., Elzinga M., Tu A.T.;
RT "Amino acid sequence and disulfide bond assignment of myotoxin a isolated
RT from the venom of Prairie rattlesnake (Crotalus viridis viridis).";
RL Biochemistry 18:678-684(1979).
RN [2]
RP PROTEIN SEQUENCE, AND MASS SPECTROMETRY.
RC TISSUE=Venom;
RX PubMed=2253781; DOI=10.1016/0014-5793(90)81325-i;
RA Griffin P.R., Aird S.D.;
RT "A new small myotoxin from the venom of the prairie rattlesnake (Crotalus
RT viridis viridis).";
RL FEBS Lett. 274:43-47(1990).
RN [3]
RP PROTEIN SEQUENCE.
RC TISSUE=Venom;
RX PubMed=2048143; DOI=10.1016/0041-0101(91)90111-4;
RA Aird S.D., Kruggel W.G., Kaiser I.I.;
RT "Multiple myotoxin sequences from the venom of a single prairie rattlesnake
RT (Crotalus viridis viridis).";
RL Toxicon 29:265-268(1991).
RN [4]
RP FUNCTION, AND SYNTHESIS.
RX PubMed=1329655; DOI=10.1016/0003-9861(92)90418-v;
RA Baker B., Utaisincharoen P., Tu A.T.;
RT "Structure-function relationship of myotoxin a using peptide fragments.";
RL Arch. Biochem. Biophys. 298:325-331(1992).
CC -!- FUNCTION: Cationic peptide that possesses multiple functions. It acts
CC as a cell-penetrating peptide (CPP), and as a potent voltage-gated
CC potassium channel (Kv) inhibitor. It exhibits antimicrobial activities,
CC hind limb paralysis, and severe muscle necrosis by a non-enzymatic
CC mechanism (By similarity). It also binds to sarcoplasmic reticulum
CC calcium-ATPase (PubMed:1329655). {ECO:0000250|UniProtKB:Q9PWF3,
CC ECO:0000269|PubMed:1329655}.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:570412}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:570412}.
CC -!- MASS SPECTROMETRY: Mass=4824.0; Mass_error=1.45; Method=FAB;
CC Evidence={ECO:0000269|PubMed:2253781};
CC -!- TOXIC DOSE: LD(50) is 3.0 mg/kg by intramuscular injection.
CC -!- SIMILARITY: Belongs to the crotamine-myotoxin family. {ECO:0000305}.
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DR PIR; A01736; MXRSMV.
DR PIR; C39560; C39560.
DR AlphaFoldDB; P01476; -.
DR SMR; P01476; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0044564; P:envenomation resulting in occlusion of the pore of voltage-gated potassium channel in another organism; ISS:UniProtKB.
DR Gene3D; 2.20.20.10; -; 1.
DR InterPro; IPR023355; Myo_ane_neurotoxin_sf.
DR InterPro; IPR000881; Myotoxin.
DR Pfam; PF00819; Myotoxins; 1.
DR PRINTS; PR00283; MYOTOXIN.
DR PROSITE; PS00459; MYOTOXINS_1; 1.
DR PROSITE; PS51345; MYOTOXINS_2; 1.
PE 1: Evidence at protein level;
KW Antimicrobial; Direct protein sequencing; Disulfide bond;
KW Ion channel impairing toxin; Myotoxin; Neurotoxin;
KW Potassium channel impairing toxin; Secreted; Toxin;
KW Voltage-gated potassium channel impairing toxin.
FT CHAIN 1..42
FT /note="Myotoxin-A"
FT /evidence="ECO:0000269|PubMed:570412"
FT /id="PRO_0000221566"
FT DISULFID 4..36
FT /evidence="ECO:0000269|PubMed:570412"
FT DISULFID 11..30
FT /evidence="ECO:0000269|PubMed:570412"
FT DISULFID 18..37
FT /evidence="ECO:0000269|PubMed:570412"
FT VARIANT 19
FT /note="I -> T (in fraction 5 and in 10% of fraction 2)"
FT VARIANT 25
FT /note="L -> F (in fraction 5 and in 10% of fraction 2)"
FT VARIANT 25
FT /note="L -> K (in 90% of fraction 2, 20% of fraction 3 and
FT 78% of fraction 4)"
FT VARIANT 33
FT /note="K -> R (in fraction 5)"
SQ SEQUENCE 42 AA; 4828 MW; A27AD67EE6AE69D0 CRC64;
YKQCHKKGGH CFPKEKICIP PSSDLGKMDC RWKWKCCKKG SG
