ID   AROC2_SOLLC             Reviewed;         431 AA.
AC   Q42885;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Chorismate synthase 2, chloroplastic;
DE            EC=4.2.3.5;
DE   AltName: Full=5-enolpyruvylshikimate-3-phosphate phospholyase 2;
DE   Flags: Precursor;
GN   Name=CS2;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. UC82B;
RX   PubMed=8251624; DOI=10.1007/bf00021526;
RA   Goerlach J., Schmid J., Amrheim N.;
RT   "Differential expression of tomato (Lycopersicon esculentum L.) genes
RT   encoding shikimate pathway isoenzymes. II. Chorismate synthase.";
RL   Plant Mol. Biol. 23:707-716(1993).
CC   -!- FUNCTION: Catalyzes the last common step of the biosynthesis of
CC       aromatic amino acids, produced via the shikimic acid pathway.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate +
CC         phosphate; Xref=Rhea:RHEA:21020, ChEBI:CHEBI:29748,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57701; EC=4.2.3.5;
CC   -!- COFACTOR:
CC       Name=FMNH2; Xref=ChEBI:CHEBI:57618;
CC   -!- PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis;
CC       chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step
CC       7/7.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- TISSUE SPECIFICITY: Predominantly expressed in flowers and roots and,
CC       to a lesser extent, in stems, leaves, and cotyledons.
CC   -!- SIMILARITY: Belongs to the chorismate synthase family. {ECO:0000305}.
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DR   EMBL; Z21791; CAA79854.1; -; mRNA.
DR   PIR; S40409; S40409.
DR   RefSeq; NP_001234411.1; NM_001247482.1.
DR   AlphaFoldDB; Q42885; -.
DR   SMR; Q42885; -.
DR   STRING; 4081.Solyc04g009620.2.1; -.
DR   PaxDb; Q42885; -.
DR   PRIDE; Q42885; -.
DR   EnsemblPlants; Solyc04g009620.3.1; Solyc04g009620.3.1; Solyc04g009620.3.
DR   GeneID; 544151; -.
DR   Gramene; Solyc04g009620.3.1; Solyc04g009620.3.1; Solyc04g009620.3.
DR   KEGG; sly:544151; -.
DR   eggNOG; KOG4492; Eukaryota.
DR   HOGENOM; CLU_034547_0_1_1; -.
DR   InParanoid; Q42885; -.
DR   OMA; PCIVQRA; -.
DR   OrthoDB; 826475at2759; -.
DR   PhylomeDB; Q42885; -.
DR   UniPathway; UPA00053; UER00090.
DR   Proteomes; UP000004994; Chromosome 4.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004107; F:chorismate synthase activity; IBA:GO_Central.
DR   GO; GO:0010181; F:FMN binding; IBA:GO_Central.
DR   GO; GO:0009073; P:aromatic amino acid family biosynthetic process; IBA:GO_Central.
DR   GO; GO:0008652; P:cellular amino acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0009423; P:chorismate biosynthetic process; IBA:GO_Central.
DR   CDD; cd07304; Chorismate_synthase; 1.
DR   Gene3D; 3.60.150.10; -; 1.
DR   HAMAP; MF_00300; Chorismate_synth; 1.
DR   InterPro; IPR000453; Chorismate_synth.
DR   InterPro; IPR035904; Chorismate_synth_AroC_sf.
DR   InterPro; IPR020541; Chorismate_synthase_CS.
DR   PANTHER; PTHR21085; PTHR21085; 1.
DR   Pfam; PF01264; Chorismate_synt; 1.
DR   PIRSF; PIRSF001456; Chorismate_synth; 1.
DR   SUPFAM; SSF103263; SSF103263; 1.
DR   TIGRFAMs; TIGR00033; aroC; 1.
DR   PROSITE; PS00787; CHORISMATE_SYNTHASE_1; 1.
DR   PROSITE; PS00788; CHORISMATE_SYNTHASE_2; 1.
DR   PROSITE; PS00789; CHORISMATE_SYNTHASE_3; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid biosynthesis; Aromatic amino acid biosynthesis; Chloroplast;
KW   Lyase; Plastid; Reference proteome; Transit peptide.
FT   TRANSIT         1..48
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           49..431
FT                   /note="Chorismate synthase 2, chloroplastic"
FT                   /id="PRO_0000002297"
FT   REGION          93..141
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        93..108
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        109..141
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   431 AA;  46871 MW;  D72D26C0F4F6B003 CRC64;
     MASSMLTKQF LGAPFSSFGS GQQPSKLCSS NLRFPTHRSQ PKRLEIQAAG NTFGNYFRVT
     TFGESHGGGV GCIIDGCPPR LPLSESDMQV ELDRRRPGQS RITTPRKETD TCKISSGTAD
     GLTTGSPIKV EVPNTDQRGN DYSEMSLAYR PSHADATYDF KYGVRSVQGG GRSSARETIG
     RVAAGAVAKK ILKLYSGTEI LAYVSQVHNV VLPEDLVDNQ IVTLEQIESN IVRCPNPEYA
     EKMIGAIDYV RVRGDSVGGV VTCIVRNVPR GLGTPVFDKL EAELAKACMS LPATKGFEFG
     SGFAGTFMTG SEHNDEFFMD EHDQIRTKTN RSGGIQGGIS NGEIINMRVA FKPTSTIARK
     QHTVSRDKHE TELIARGRHD PCVVPRAVPM VEAMVALVLV DQLMTQYAQC MLFPVNLTLQ
     EPLQPSTTKS A