MZT1_HUMAN
ID MZT1_HUMAN Reviewed; 82 AA.
AC Q08AG7; Q5W0P5;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Mitotic-spindle organizing protein 1;
DE AltName: Full=Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 1;
GN Name=MZT1; Synonyms=C13orf37, MOZART1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057823; DOI=10.1038/nature02379;
RA Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S.,
RA Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P.,
RA Ambrose K.D., Andrews D.T., Ashwell R.I.S., Babbage A.K., Bagguley C.L.,
RA Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P.,
RA Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C.,
RA Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P.,
RA Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L.,
RA Frankish A.G., Frankland J., French L., Garner P., Garnett J.,
RA Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M.,
RA Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D.,
RA Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D.,
RA Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S.,
RA Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R.,
RA Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W.,
RA Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P.,
RA Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L.,
RA Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R.,
RA Rogers J., Ross M.T.;
RT "The DNA sequence and analysis of human chromosome 13.";
RL Nature 428:522-528(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLY-5.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH TUBG1.
RX PubMed=20360068; DOI=10.1126/science.1181348;
RA Hutchins J.R., Toyoda Y., Hegemann B., Poser I., Heriche J.K., Sykora M.M.,
RA Augsburg M., Hudecz O., Buschhorn B.A., Bulkescher J., Conrad C.,
RA Comartin D., Schleiffer A., Sarov M., Pozniakovsky A., Slabicki M.M.,
RA Schloissnig S., Steinmacher I., Leuschner M., Ssykor A., Lawo S.,
RA Pelletier L., Stark H., Nasmyth K., Ellenberg J., Durbin R., Buchholz F.,
RA Mechtler K., Hyman A.A., Peters J.M.;
RT "Systematic analysis of human protein complexes identifies chromosome
RT segregation proteins.";
RL Science 328:593-599(2010).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Required for gamma-tubulin complex recruitment to the
CC centrosome. {ECO:0000269|PubMed:20360068}.
CC -!- SUBUNIT: Part of the gamma-tubulin complex. Interacts with TUBG1.
CC {ECO:0000269|PubMed:20360068}.
CC -!- INTERACTION:
CC Q08AG7; Q9BW66: CINP; NbExp=5; IntAct=EBI-2637198, EBI-739784;
CC Q08AG7; Q8WWM9: CYGB; NbExp=3; IntAct=EBI-2637198, EBI-6309037;
CC Q08AG7; Q6ZMU5: TRIM72; NbExp=3; IntAct=EBI-2637198, EBI-2341648;
CC Q08AG7; P23258: TUBG1; NbExp=4; IntAct=EBI-2637198, EBI-302589;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000269|PubMed:20360068}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:20360068}.
CC -!- SIMILARITY: Belongs to the MOZART1 family. {ECO:0000305}.
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DR EMBL; AL356754; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL138695; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC125183; AAI25184.1; -; mRNA.
DR CCDS; CCDS31990.1; -.
DR RefSeq; NP_001065243.1; NM_001071775.2.
DR PDB; 6L81; X-ray; 2.20 A; B/D=1-82.
DR PDB; 6M33; X-ray; 3.29 A; B=1-82.
DR PDB; 6X0U; EM; 3.60 A; A/C=1-82.
DR PDB; 7AS4; EM; 4.13 A; 5/6=1-82.
DR PDB; 7QJ0; EM; 5.32 A; b/m=1-82.
DR PDB; 7QJ1; EM; 7.00 A; b/m=1-82.
DR PDB; 7QJ2; EM; 8.60 A; b/k/m=1-82.
DR PDB; 7QJ3; EM; 7.60 A; b/m/o=1-82.
DR PDB; 7QJ4; EM; 9.00 A; b/k/m/o=1-82.
DR PDB; 7QJ5; EM; 8.70 A; b/d/g/i/k/m=1-82.
DR PDB; 7QJ6; EM; 7.80 A; b/d/i/k/m=1-82.
DR PDB; 7QJ7; EM; 8.70 A; b/d/g/i/k/m=1-82.
DR PDB; 7QJ8; EM; 8.70 A; b/d/i/k/m=1-82.
DR PDB; 7QJ9; EM; 8.10 A; b/d/i/k/m=1-82.
DR PDB; 7QJA; EM; 9.20 A; b/d/g/i/k/m=1-82.
DR PDB; 7QJB; EM; 9.20 A; b/d/i/k/m=1-82.
DR PDB; 7QJC; EM; 16.10 A; b/d/g/i/k/m=1-82.
DR PDB; 7QJD; EM; 7.10 A; b/d/g/i/k/m/o=1-82.
DR PDBsum; 6L81; -.
DR PDBsum; 6M33; -.
DR PDBsum; 6X0U; -.
DR PDBsum; 7AS4; -.
DR PDBsum; 7QJ0; -.
DR PDBsum; 7QJ1; -.
DR PDBsum; 7QJ2; -.
DR PDBsum; 7QJ3; -.
DR PDBsum; 7QJ4; -.
DR PDBsum; 7QJ5; -.
DR PDBsum; 7QJ6; -.
DR PDBsum; 7QJ7; -.
DR PDBsum; 7QJ8; -.
DR PDBsum; 7QJ9; -.
DR PDBsum; 7QJA; -.
DR PDBsum; 7QJB; -.
DR PDBsum; 7QJC; -.
DR PDBsum; 7QJD; -.
DR AlphaFoldDB; Q08AG7; -.
DR SMR; Q08AG7; -.
DR BioGRID; 136335; 65.
DR IntAct; Q08AG7; 45.
DR STRING; 9606.ENSP00000367049; -.
DR iPTMnet; Q08AG7; -.
DR PhosphoSitePlus; Q08AG7; -.
DR BioMuta; MZT1; -.
DR DMDM; 189037059; -.
DR EPD; Q08AG7; -.
DR jPOST; Q08AG7; -.
DR MassIVE; Q08AG7; -.
DR MaxQB; Q08AG7; -.
DR PaxDb; Q08AG7; -.
DR PeptideAtlas; Q08AG7; -.
DR PRIDE; Q08AG7; -.
DR ProteomicsDB; 58669; -.
DR TopDownProteomics; Q08AG7; -.
DR Antibodypedia; 71297; 31 antibodies from 12 providers.
DR DNASU; 440145; -.
DR Ensembl; ENST00000377818.4; ENSP00000367049.3; ENSG00000204899.6.
DR GeneID; 440145; -.
DR KEGG; hsa:440145; -.
DR MANE-Select; ENST00000377818.4; ENSP00000367049.3; NM_001071775.3; NP_001065243.1.
DR UCSC; uc001viu.3; human.
DR CTD; 440145; -.
DR GeneCards; MZT1; -.
DR HGNC; HGNC:33830; MZT1.
DR HPA; ENSG00000204899; Low tissue specificity.
DR MIM; 613448; gene.
DR neXtProt; NX_Q08AG7; -.
DR OpenTargets; ENSG00000204899; -.
DR PharmGKB; PA165505227; -.
DR VEuPathDB; HostDB:ENSG00000204899; -.
DR eggNOG; ENOG502S6UI; Eukaryota.
DR GeneTree; ENSGT00390000005954; -.
DR HOGENOM; CLU_160285_0_0_1; -.
DR InParanoid; Q08AG7; -.
DR OMA; LSICVGM; -.
DR OrthoDB; 1645115at2759; -.
DR PhylomeDB; Q08AG7; -.
DR TreeFam; TF328444; -.
DR PathwayCommons; Q08AG7; -.
DR Reactome; R-HSA-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-HSA-380320; Recruitment of NuMA to mitotic centrosomes.
DR SignaLink; Q08AG7; -.
DR BioGRID-ORCS; 440145; 608 hits in 1053 CRISPR screens.
DR ChiTaRS; MZT1; human.
DR GenomeRNAi; 440145; -.
DR Pharos; Q08AG7; Tdark.
DR PRO; PR:Q08AG7; -.
DR Proteomes; UP000005640; Chromosome 13.
DR RNAct; Q08AG7; protein.
DR Bgee; ENSG00000204899; Expressed in cerebellar vermis and 184 other tissues.
DR Genevisible; Q08AG7; HS.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0000931; C:gamma-tubulin large complex; IDA:UniProtKB.
DR GO; GO:0031021; C:interphase microtubule organizing center; IBA:GO_Central.
DR GO; GO:0005819; C:spindle; IDA:UniProtKB.
DR GO; GO:0033566; P:gamma-tubulin complex localization; IMP:UniProtKB.
DR GO; GO:0051415; P:microtubule nucleation by interphase microtubule organizing center; IBA:GO_Central.
DR GO; GO:0090307; P:mitotic spindle assembly; IBA:GO_Central.
DR InterPro; IPR022214; MZT1.
DR PANTHER; PTHR28520; PTHR28520; 1.
DR Pfam; PF12554; MOZART1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cytoplasm; Cytoskeleton; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..82
FT /note="Mitotic-spindle organizing protein 1"
FT /id="PRO_0000337015"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT VARIANT 5
FT /note="S -> G (in dbSNP:rs1465896)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_043562"
FT HELIX 16..34
FT /evidence="ECO:0007829|PDB:6L81"
FT HELIX 41..52
FT /evidence="ECO:0007829|PDB:6L81"
FT HELIX 57..69
FT /evidence="ECO:0007829|PDB:6L81"
FT TURN 70..73
FT /evidence="ECO:0007829|PDB:6L81"
SQ SEQUENCE 82 AA; 8479 MW; EA48466A00CF23C7 CRC64;
MASSSGAGAA AAAAAANLNA VRETMDVLLE ISRILNTGLD METLSICVRL CEQGINPEAL
SSVIKELRKA TEALKAAENM TS
