MZT2B_HUMAN
ID MZT2B_HUMAN Reviewed; 158 AA.
AC Q6NZ67; Q96CG4;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Mitotic-spindle organizing protein 2B;
DE AltName: Full=Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 2B;
GN Name=MZT2B; Synonyms=FAM128B, MOZART2B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-34, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [5]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH TUBG1.
RX PubMed=20360068; DOI=10.1126/science.1181348;
RA Hutchins J.R., Toyoda Y., Hegemann B., Poser I., Heriche J.K., Sykora M.M.,
RA Augsburg M., Hudecz O., Buschhorn B.A., Bulkescher J., Conrad C.,
RA Comartin D., Schleiffer A., Sarov M., Pozniakovsky A., Slabicki M.M.,
RA Schloissnig S., Steinmacher I., Leuschner M., Ssykor A., Lawo S.,
RA Pelletier L., Stark H., Nasmyth K., Ellenberg J., Durbin R., Buchholz F.,
RA Mechtler K., Hyman A.A., Peters J.M.;
RT "Systematic analysis of human protein complexes identifies chromosome
RT segregation proteins.";
RL Science 328:593-599(2010).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- SUBUNIT: Part of the gamma-tubulin complex. Interacts with TUBG1.
CC {ECO:0000269|PubMed:20360068}.
CC -!- INTERACTION:
CC Q6NZ67; P23258: TUBG1; NbExp=4; IntAct=EBI-1052566, EBI-302589;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000269|PubMed:20360068}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:20360068}.
CC -!- SIMILARITY: Belongs to the MOZART2 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CH471263; EAW55603.1; -; Genomic_DNA.
DR EMBL; BC014255; AAH14255.2; -; mRNA.
DR EMBL; BC066296; AAH66296.1; -; mRNA.
DR CCDS; CCDS2157.1; -.
DR RefSeq; NP_079305.2; NM_025029.4.
DR AlphaFoldDB; Q6NZ67; -.
DR SMR; Q6NZ67; -.
DR BioGRID; 123111; 79.
DR IntAct; Q6NZ67; 40.
DR MINT; Q6NZ67; -.
DR STRING; 9606.ENSP00000281871; -.
DR iPTMnet; Q6NZ67; -.
DR PhosphoSitePlus; Q6NZ67; -.
DR BioMuta; MZT2B; -.
DR EPD; Q6NZ67; -.
DR jPOST; Q6NZ67; -.
DR MassIVE; Q6NZ67; -.
DR MaxQB; Q6NZ67; -.
DR PaxDb; Q6NZ67; -.
DR PeptideAtlas; Q6NZ67; -.
DR PRIDE; Q6NZ67; -.
DR ProteomicsDB; 66793; -.
DR TopDownProteomics; Q6NZ67; -.
DR Antibodypedia; 77852; 20 antibodies from 10 providers.
DR DNASU; 80097; -.
DR Ensembl; ENST00000281871.11; ENSP00000281871.7; ENSG00000152082.15.
DR GeneID; 80097; -.
DR KEGG; hsa:80097; -.
DR MANE-Select; ENST00000281871.11; ENSP00000281871.7; NM_025029.5; NP_079305.2.
DR UCSC; uc002tqu.3; human.
DR CTD; 80097; -.
DR GeneCards; MZT2B; -.
DR HGNC; HGNC:25886; MZT2B.
DR HPA; ENSG00000152082; Low tissue specificity.
DR MIM; 613450; gene.
DR neXtProt; NX_Q6NZ67; -.
DR OpenTargets; ENSG00000152082; -.
DR PharmGKB; PA165696943; -.
DR VEuPathDB; HostDB:ENSG00000152082; -.
DR eggNOG; ENOG502S50R; Eukaryota.
DR GeneTree; ENSGT00390000014845; -.
DR HOGENOM; CLU_105461_0_0_1; -.
DR InParanoid; Q6NZ67; -.
DR PhylomeDB; Q6NZ67; -.
DR TreeFam; TF333013; -.
DR PathwayCommons; Q6NZ67; -.
DR Reactome; R-HSA-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-HSA-380320; Recruitment of NuMA to mitotic centrosomes.
DR SignaLink; Q6NZ67; -.
DR BioGRID-ORCS; 80097; 8 hits in 1031 CRISPR screens.
DR ChiTaRS; MZT2B; human.
DR GenomeRNAi; 80097; -.
DR Pharos; Q6NZ67; Tdark.
DR PRO; PR:Q6NZ67; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q6NZ67; protein.
DR Bgee; ENSG00000152082; Expressed in apex of heart and 194 other tissues.
DR ExpressionAtlas; Q6NZ67; baseline and differential.
DR Genevisible; Q6NZ67; HS.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0000931; C:gamma-tubulin large complex; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005819; C:spindle; IDA:UniProtKB.
DR InterPro; IPR024332; MOZART2.
DR PANTHER; PTHR28578; PTHR28578; 1.
DR Pfam; PF12926; MOZART2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Phosphoprotein; Reference proteome.
FT CHAIN 1..158
FT /note="Mitotic-spindle organizing protein 2B"
FT /id="PRO_0000338988"
FT REGION 84..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 93..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 34
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19690332"
FT MOD_RES 152
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231"
FT VARIANT 118
FT /note="A -> V (in dbSNP:rs1043059)"
FT /id="VAR_043853"
FT VARIANT 155
FT /note="R -> Q (in dbSNP:rs1043152)"
FT /id="VAR_043854"
SQ SEQUENCE 158 AA; 16226 MW; 97DE6BDDE2881289 CRC64;
MAAQGVGPGP GSAAPPGLEA ARQKLALRRK KVLSTEEMEL YELAQAAGGA IDPDVFKILV
DLLKLNVAPL AVFQMLKSMC AGQRLASEPQ DPAAVSLPTS SVPETRGRNK GSAALGGALA
LAERSSREGS SQRMPRQPSA TRLPKGGGPG KSPTRGST
