MZT2_MOUSE
ID MZT2_MOUSE Reviewed; 159 AA.
AC Q9CQ25;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Mitotic-spindle organizing protein 2;
DE AltName: Full=Mitotic-spindle organizing protein associated with a ring of gamma-tubulin 2B;
GN Name=Mzt2; Synonyms=Fam128b, Mozart2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH TUBG1.
RX PubMed=20360068; DOI=10.1126/science.1181348;
RA Hutchins J.R., Toyoda Y., Hegemann B., Poser I., Heriche J.K., Sykora M.M.,
RA Augsburg M., Hudecz O., Buschhorn B.A., Bulkescher J., Conrad C.,
RA Comartin D., Schleiffer A., Sarov M., Pozniakovsky A., Slabicki M.M.,
RA Schloissnig S., Steinmacher I., Leuschner M., Ssykor A., Lawo S.,
RA Pelletier L., Stark H., Nasmyth K., Ellenberg J., Durbin R., Buchholz F.,
RA Mechtler K., Hyman A.A., Peters J.M.;
RT "Systematic analysis of human protein complexes identifies chromosome
RT segregation proteins.";
RL Science 328:593-599(2010).
RN [5]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-111, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- SUBUNIT: Part of the gamma-tubulin complex. Interacts with TUBG1.
CC {ECO:0000269|PubMed:20360068}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000269|PubMed:20360068}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:20360068}.
CC -!- SIMILARITY: Belongs to the MOZART2 family. {ECO:0000305}.
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DR EMBL; AK010544; BAB27018.1; -; mRNA.
DR EMBL; AK011255; BAB27497.1; -; mRNA.
DR EMBL; BC027550; AAH27550.1; -; mRNA.
DR CCDS; CCDS27979.1; -.
DR RefSeq; NP_083630.1; NM_029354.2.
DR RefSeq; XP_006522651.1; XM_006522588.2.
DR AlphaFoldDB; Q9CQ25; -.
DR SMR; Q9CQ25; -.
DR BioGRID; 215139; 25.
DR IntAct; Q9CQ25; 18.
DR MINT; Q9CQ25; -.
DR STRING; 10090.ENSMUSP00000023351; -.
DR iPTMnet; Q9CQ25; -.
DR PhosphoSitePlus; Q9CQ25; -.
DR EPD; Q9CQ25; -.
DR MaxQB; Q9CQ25; -.
DR PaxDb; Q9CQ25; -.
DR PeptideAtlas; Q9CQ25; -.
DR PRIDE; Q9CQ25; -.
DR ProteomicsDB; 286132; -.
DR TopDownProteomics; Q9CQ25; -.
DR DNASU; 72083; -.
DR Ensembl; ENSMUST00000023351; ENSMUSP00000023351; ENSMUSG00000022671.
DR Ensembl; ENSMUST00000117136; ENSMUSP00000113832; ENSMUSG00000022671.
DR GeneID; 72083; -.
DR KEGG; mmu:72083; -.
DR UCSC; uc007yhv.1; mouse.
DR CTD; 72083; -.
DR MGI; MGI:1922845; Mzt2.
DR VEuPathDB; HostDB:ENSMUSG00000022671; -.
DR eggNOG; ENOG502SCQ3; Eukaryota.
DR GeneTree; ENSGT00390000014845; -.
DR HOGENOM; CLU_105461_0_0_1; -.
DR InParanoid; Q9CQ25; -.
DR OMA; MCAGQRA; -.
DR OrthoDB; 1453946at2759; -.
DR PhylomeDB; Q9CQ25; -.
DR TreeFam; TF333013; -.
DR Reactome; R-MMU-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-MMU-380320; Recruitment of NuMA to mitotic centrosomes.
DR BioGRID-ORCS; 72083; 7 hits in 73 CRISPR screens.
DR ChiTaRS; Mzt2; mouse.
DR PRO; PR:Q9CQ25; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9CQ25; protein.
DR Bgee; ENSMUSG00000022671; Expressed in spermatid and 249 other tissues.
DR ExpressionAtlas; Q9CQ25; baseline and differential.
DR Genevisible; Q9CQ25; MM.
DR GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0000931; C:gamma-tubulin large complex; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005819; C:spindle; ISS:UniProtKB.
DR InterPro; IPR024332; MOZART2.
DR PANTHER; PTHR28578; PTHR28578; 1.
DR Pfam; PF12926; MOZART2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Methylation; Phosphoprotein; Reference proteome.
FT CHAIN 1..159
FT /note="Mitotic-spindle organizing protein 2"
FT /id="PRO_0000338986"
FT REGION 87..159
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 91..109
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 111
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 153
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6NZ67"
SQ SEQUENCE 159 AA; 16524 MW; D3B6F413E4DE87A0 CRC64;
MAAAAAAAGG AALAVSTGLE TATLQKLALR RKKVLGAEEM ELYELAQAAG AAIDPDVFKI
LVDLLNLNVA PLAVFQMLKS MCAGQRLASD PQDSVPISLS TSTSETRGRN RGGPILGNVT
ISAERGSRER PIQRMPRQPS ATRLPKVGGS GKSNSRSSP
