ID   N1_VACCC                Reviewed;         117 AA.
AC   P21054;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Protein N1;
GN   ORFNames=N1L;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
RX   PubMed=17123957; DOI=10.1110/ps.062454707;
RA   Aoyagi M., Zhai D., Jin C., Aleshin A.E., Stec B., Reed J.C.,
RA   Liddington R.C.;
RT   "Vaccinia virus N1L protein resembles a B cell lymphoma-2 (Bcl-2) family
RT   protein.";
RL   Protein Sci. 16:118-124(2007).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
RX   PubMed=17485524; DOI=10.1099/vir.0.82772-0;
RA   Cooray S., Bahar M.W., Abrescia N.G., McVey C.E., Bartlett N.W., Chen R.A.,
RA   Stuart D.I., Grimes J.M., Smith G.L.;
RT   "Functional and structural studies of the vaccinia virus virulence factor
RT   N1 reveal a Bcl-2-like anti-apoptotic protein.";
RL   J. Gen. Virol. 88:1656-1666(2007).
CC   -!- FUNCTION: Bcl-2-like protein which contributes to virulence by
CC       preventing host NF-kappa-B activation in response to pro-inflammatory
CC       stimuli such as TNF-alpha or IL1B. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae N1 protein family. {ECO:0000305}.
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DR   EMBL; M35027; AAA48001.1; -; Genomic_DNA.
DR   PIR; E42504; E42504.
DR   PDB; 2I39; X-ray; 2.20 A; A/B/C/D/E/F=1-117.
DR   PDB; 2UXE; X-ray; 2.90 A; A/B/C/D/E/F=1-117.
DR   PDB; 4BBB; X-ray; 3.09 A; A/B/C/D/E/F=1-117.
DR   PDB; 4BBC; X-ray; 3.10 A; A/B/C/D/E/F=1-117.
DR   PDB; 4BBD; X-ray; 3.00 A; A/B/C/D/E/F=1-117.
DR   PDBsum; 2I39; -.
DR   PDBsum; 2UXE; -.
DR   PDBsum; 4BBB; -.
DR   PDBsum; 4BBC; -.
DR   PDBsum; 4BBD; -.
DR   SMR; P21054; -.
DR   PRIDE; P21054; -.
DR   EvolutionaryTrace; P21054; -.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0039526; P:modulation by virus of host apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0039644; P:suppression by virus of host NF-kappaB cascade; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.437.20; -; 1.
DR   InterPro; IPR009353; Orthopox_N1.
DR   InterPro; IPR022819; Poxvirus_Bcl-2-like.
DR   InterPro; IPR043018; Poxvirus_sf.
DR   Pfam; PF06227; Poxvirus; 1.
DR   PIRSF; PIRSF003784; VAC_N1L; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Early protein; Host-virus interaction;
KW   Inhibition of host NF-kappa-B by virus;
KW   Modulation of host cell apoptosis by virus; Reference proteome;
KW   Viral immunoevasion.
FT   CHAIN           1..117
FT                   /note="Protein N1"
FT                   /id="PRO_0000099632"
FT   HELIX           2..14
FT                   /evidence="ECO:0007829|PDB:4BBD"
FT   HELIX           17..19
FT                   /evidence="ECO:0007829|PDB:4BBD"
FT   HELIX           24..28
FT                   /evidence="ECO:0007829|PDB:4BBD"
FT   HELIX           29..33
FT                   /evidence="ECO:0007829|PDB:4BBD"
FT   HELIX           36..49
FT                   /evidence="ECO:0007829|PDB:4BBD"
FT   HELIX           53..59
FT                   /evidence="ECO:0007829|PDB:4BBD"
FT   HELIX           66..83
FT                   /evidence="ECO:0007829|PDB:4BBD"
FT   HELIX           91..102
FT                   /evidence="ECO:0007829|PDB:4BBD"
FT   HELIX           103..105
FT                   /evidence="ECO:0007829|PDB:4BBD"
FT   HELIX           106..113
FT                   /evidence="ECO:0007829|PDB:4BBD"
SQ   SEQUENCE   117 AA;  13962 MW;  9050C1FECEB1B90B CRC64;
     MRTLLIRYIL WRNDNDQTYY NDDFKKLMLL DELVDDGDVC TLIKNMRMTL SDGPLLDRLN
     QPVNNIEDAK RMIAISAKVA RDIGERSEIR WEESFTILFR MIETYFDDLM IDLYGEK