ID   N1_VACCW                Reviewed;         117 AA.
AC   P17361; Q76ZY1;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Protein N1;
GN   OrderedLocusNames=VACWR028; ORFNames=N1L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2849238; DOI=10.1016/s0042-6822(88)90115-8;
RA   Kotwal G.J., Moss B.;
RT   "Analysis of a large cluster of nonessential genes deleted from a vaccinia
RT   virus terminal transposition mutant.";
RL   Virology 167:524-537(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION.
RX   PubMed=22194685; DOI=10.1371/journal.ppat.1002430;
RA   Maluquer de Motes C., Cooray S., Ren H., Almeida G.M., McGourty K.,
RA   Bahar M.W., Stuart D.I., Grimes J.M., Graham S.C., Smith G.L.;
RT   "Inhibition of apoptosis and NF-kappaB activation by vaccinia protein N1
RT   occur via distinct binding surfaces and make different contributions to
RT   virulence.";
RL   PLoS Pathog. 7:E1002430-E1002430(2011).
CC   -!- FUNCTION: Bcl-2-like protein which contributes to virulence by
CC       preventing host NF-kappa-B activation in response to pro-inflammatory
CC       stimuli such as TNF-alpha or IL1B. {ECO:0000269|PubMed:22194685}.
CC   -!- INTERACTION:
CC       P17361; P17361: VACWR028; NbExp=2; IntAct=EBI-7115640, EBI-7115640;
CC       P17361; Q92934: BAD; Xeno; NbExp=2; IntAct=EBI-7115640, EBI-700771;
CC       P17361; Q07812: BAX; Xeno; NbExp=3; IntAct=EBI-7115640, EBI-516580;
CC       P17361; P55957: BID; Xeno; NbExp=2; IntAct=EBI-7115640, EBI-519672;
CC   -!- SIMILARITY: Belongs to the poxviridae N1 protein family. {ECO:0000305}.
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DR   EMBL; M22812; AAA69608.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89307.1; -; Genomic_DNA.
DR   PIR; H33348; WZVZB8.
DR   SMR; P17361; -.
DR   IntAct; P17361; 4.
DR   MINT; P17361; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0039526; P:modulation by virus of host apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0039644; P:suppression by virus of host NF-kappaB cascade; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.437.20; -; 1.
DR   InterPro; IPR009353; Orthopox_N1.
DR   InterPro; IPR022819; Poxvirus_Bcl-2-like.
DR   InterPro; IPR043018; Poxvirus_sf.
DR   Pfam; PF06227; Poxvirus; 1.
DR   PIRSF; PIRSF003784; VAC_N1L; 1.
PE   1: Evidence at protein level;
KW   Early protein; Host-virus interaction;
KW   Inhibition of host NF-kappa-B by virus;
KW   Modulation of host cell apoptosis by virus; Reference proteome;
KW   Viral immunoevasion.
FT   CHAIN           1..117
FT                   /note="Protein N1"
FT                   /id="PRO_0000099633"
FT   CONFLICT        23
FT                   /note="N -> D (in Ref. 2; AAO89307)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   117 AA;  13961 MW;  9E5E2110CE51B3A1 CRC64;
     MRTLLIRYIL WRNDNDQTYY NDNFKKLMLL DELVDDGDVC TLIKNMRMTL SDGPLLDRLN
     QPVNNIEDAK RMIAISAKVA RDIGERSEIR WEESFTILFR MIETYFDDLM IDLYGEK