ID   N24_MEDTR               Reviewed;         234 AA.
AC   O24088;
DT   09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=CASP-like protein N24;
DE   AltName: Full=CASP-like protein 4A1;
DE            Short=MtCASPL4A1;
DE   AltName: Full=Nodulin 24;
DE            Short=MtN24;
GN   Name=N24;
OS   Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX   NCBI_TaxID=3880;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION DURING NODULATION.
RC   STRAIN=cv. Jemalong J5; TISSUE=Root nodule;
RX   PubMed=8634476; DOI=10.1094/mpmi-9-0233;
RA   Gamas P., de Carvalho Niebel F., Lescure N., Cullimore J.;
RT   "Use of a subtractive hybridization approach to identify new Medicago
RT   truncatula genes induced during root nodule development.";
RL   Mol. Plant Microbe Interact. 9:233-242(1996).
RN   [2]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=24920445; DOI=10.1104/pp.114.239137;
RA   Roppolo D., Boeckmann B., Pfister A., Boutet E., Rubio M.C.,
RA   Denervaud-Tendon V., Vermeer J.E., Gheyselinck J., Xenarios I., Geldner N.;
RT   "Functional and evolutionary analysis of the CASPARIAN STRIP MEMBRANE
RT   DOMAIN PROTEIN family.";
RL   Plant Physiol. 165:1709-1722(2014).
CC   -!- SUBUNIT: Homodimer and heterodimers. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- INDUCTION: During Rhizobium meliloti-mediated root nodulation.
CC       {ECO:0000269|PubMed:8634476}.
CC   -!- SIMILARITY: Belongs to the Casparian strip membrane proteins (CASP)
CC       family. {ECO:0000305}.
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DR   EMBL; Y15290; CAA75572.1; -; mRNA.
DR   RefSeq; XP_013450803.1; XM_013595349.1.
DR   AlphaFoldDB; O24088; -.
DR   ProMEX; O24088; -.
DR   EnsemblPlants; KEH24843; KEH24843; MTR_6g007160.
DR   GeneID; 25495170; -.
DR   Gramene; KEH24843; KEH24843; MTR_6g007160.
DR   HOGENOM; CLU_048961_5_0_1; -.
DR   OrthoDB; 1543616at2759; -.
DR   ExpressionAtlas; O24088; differential.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR006702; CASP_dom.
DR   Pfam; PF04535; DUF588; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Glycoprotein; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..234
FT                   /note="CASP-like protein N24"
FT                   /id="PRO_0000391510"
FT   TOPO_DOM        1..65
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        87..128
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        129..149
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        150..169
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..189
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        190..210
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        211..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        232..234
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        9..24
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        98
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        206
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   234 AA;  26529 MW;  C78DBE19296C3ACD CRC64;
     MSTINIESLD QDTKDEPKEQ TQEDNNEVLK VLEEKINMMD PKNTSGDTEV VITNFLKSKK
     EVKWYVALLV IRVFAFVFCL IAFSVLGASE QRVLVSENLT NWYSSGFTIQ TPYEFHWYKW
     DEFRYSFAAN VIGFVYSGLQ ICHLVMYLIT KKHTINPKLQ GYFNVAIDQT LAYILMSASS
     SAATAAHLLK DYWLEHGADT FIEMANASVS MSFLAFGAFA LASLVSGIIL CRFT