ID   N2_VACCW                Reviewed;         175 AA.
AC   P14357; Q76ZY0;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   23-FEB-2022, entry version 72.
DE   RecName: Full=Protein N2;
GN   OrderedLocusNames=VACWR029; ORFNames=N2L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3388767; DOI=10.1016/0042-6822(88)90667-8;
RA   Tamin A., Villarreal E.C., Weinrich S.L., Hruby D.E.;
RT   "Nucleotide sequence and molecular genetic analysis of the vaccinia virus
RT   HindIII N/M region encoding the genes responsible for resistance to alpha-
RT   amanitin.";
RL   Virology 165:141-150(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22 (TRANSPOSITION MUTANT 6/2).
RX   PubMed=2849238; DOI=10.1016/s0042-6822(88)90115-8;
RA   Kotwal G.J., Moss B.;
RT   "Analysis of a large cluster of nonessential genes deleted from a vaccinia
RT   virus terminal transposition mutant.";
RL   Virology 167:524-537(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION.
RX   PubMed=20230632; DOI=10.1186/1743-422x-7-59;
RA   Gonzalez J.M., Esteban M.;
RT   "A poxvirus Bcl-2-like gene family involved in regulation of host immune
RT   response: sequence similarity and evolutionary history.";
RL   Virol. J. 7:59-59(2010).
RN   [5]
RP   SUBCELLULAR LOCATION, AND FUNCTION.
RX   PubMed=23761407; DOI=10.1099/vir.0.054114-0;
RA   Ferguson B.J., Benfield C.T., Ren H., Lee V.H., Frazer G.L., Strnadova P.,
RA   Sumner R.P., Smith G.L.;
RT   "Vaccinia virus protein N2 is a nuclear IRF3 inhibitor that promotes
RT   virulence.";
RL   J. Gen. Virol. 94:2070-2081(2013).
CC   -!- FUNCTION: Plays a role in the inhibition of host innate immune
CC       response. Within the host nucleus, inhibits activation of interferon-
CC       beta promoter by inhibiting IRF3 activation.
CC       {ECO:0000269|PubMed:20230632, ECO:0000269|PubMed:23761407}.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:23761407}.
CC   -!- SIMILARITY: Belongs to the poxviridae N2 protein family. {ECO:0000305}.
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DR   EMBL; M20779; AAA69612.1; -; Genomic_DNA.
DR   EMBL; M22812; AAA69609.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89308.1; -; Genomic_DNA.
DR   PIR; A94377; FOVZN2.
DR   RefSeq; YP_232911.1; NC_006998.1.
DR   DNASU; 3707644; -.
DR   GeneID; 3707644; -.
DR   KEGG; vg:3707644; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
DR   GO; GO:0039548; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity; IDA:UniProtKB.
DR   InterPro; IPR022819; Poxvirus_Bcl-2-like.
DR   Pfam; PF06227; Poxvirus; 1.
PE   3: Inferred from homology;
KW   Early protein; Host nucleus; Host-virus interaction;
KW   Inhibition of host innate immune response by virus;
KW   Inhibition of host IRF3 by virus; Inhibition of host RLR pathway by virus;
KW   Reference proteome; Viral immunoevasion.
FT   CHAIN           1..175
FT                   /note="Protein N2"
FT                   /id="PRO_0000099636"
SQ   SEQUENCE   175 AA;  20852 MW;  F073F8664EA49E1A CRC64;
     MTSSAMDNNE PKVLEMVYDA TILPEGSSMD PNIMDCINRH INMCIQRTYS SSIIAILDRF
     LMMNKDELNN TQCHIIKEFM TYEQMAIDHY GGYVNAILYQ IRKRPNQHHT IDLFKRIKRT
     RYDTFKVDPV EFVKKVIGFV SILNKYKPVY SYVLYENVLY DEFKCFINYV ETKYF