N4BP1_DANRE
ID N4BP1_DANRE Reviewed; 858 AA.
AC Q1LVK9; Q6PCR0;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 3.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=NEDD4-binding protein 1 {ECO:0000250|UniProtKB:Q6A037};
DE Short=N4BP1 {ECO:0000250|UniProtKB:Q6A037};
DE EC=3.1.-.- {ECO:0000250|UniProtKB:O75113};
GN Name=n4bp1 {ECO:0000250|UniProtKB:Q6A037};
GN ORFNames=si:ch211-215n5.3 {ECO:0000303|PubMed:23594743},
GN zgc:66437 {ECO:0000303|Ref.2};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Potent suppressor of cytokine production that acts as a
CC regulator of innate immune signaling and inflammation. Acts as a key
CC negative regulator of select cytokine and chemokine responses elicited
CC by TRIF-independent Toll-like receptors (TLRs), thereby limiting
CC inflammatory cytokine responses to minor insults (By similarity). Has
CC ribonuclease activity (By similarity). {ECO:0000250|UniProtKB:O75113,
CC ECO:0000250|UniProtKB:Q6A037}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q6A037}. Nucleus,
CC nucleolus {ECO:0000250|UniProtKB:Q6A037}. Nucleus, PML body
CC {ECO:0000250|UniProtKB:Q6A037}. Note=Primarily localizes to the
CC nucleolus. Also localizes to the PML nuclear bodies.
CC {ECO:0000250|UniProtKB:Q6A037}.
CC -!- SIMILARITY: Belongs to the N4BP1 family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-10 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH59205.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; BX649644; CAK04516.1; -; Genomic_DNA.
DR EMBL; BC059205; AAH59205.1; ALT_INIT; mRNA.
DR RefSeq; NP_956081.2; NM_199787.2.
DR AlphaFoldDB; Q1LVK9; -.
DR SMR; Q1LVK9; -.
DR STRING; 7955.ENSDARP00000074624; -.
DR PaxDb; Q1LVK9; -.
DR Ensembl; ENSDART00000080174; ENSDARP00000074624; ENSDARG00000057491.
DR GeneID; 327327; -.
DR KEGG; dre:327327; -.
DR CTD; 9683; -.
DR ZFIN; ZDB-GENE-030131-5538; n4bp1.
DR eggNOG; KOG3777; Eukaryota.
DR GeneTree; ENSGT00940000158682; -.
DR HOGENOM; CLU_014137_1_0_1; -.
DR InParanoid; Q1LVK9; -.
DR OMA; KIPYKLE; -.
DR OrthoDB; 771251at2759; -.
DR PRO; PR:Q1LVK9; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 18.
DR Bgee; ENSDARG00000057491; Expressed in blastula and 21 other tissues.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0016605; C:PML body; ISS:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; ISS:UniProtKB.
DR GO; GO:0004540; F:ribonuclease activity; ISS:UniProtKB.
DR GO; GO:0043130; F:ubiquitin binding; ISS:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0001818; P:negative regulation of cytokine production; ISS:UniProtKB.
DR GO; GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR GO; GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
DR GO; GO:0045071; P:negative regulation of viral genome replication; ISS:UniProtKB.
DR GO; GO:0045088; P:regulation of innate immune response; ISS:UniProtKB.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR InterPro; IPR021869; RNase_Zc3h12_NYN.
DR Pfam; PF11977; RNase_Zc3h12a; 1.
DR SUPFAM; SSF54791; SSF54791; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Immunity; Innate immunity; Nuclease; Nucleus;
KW Reference proteome; RNA-binding.
FT CHAIN 1..858
FT /note="NEDD4-binding protein 1"
FT /id="PRO_0000301987"
FT DOMAIN 96..180
FT /note="KH-like"
FT /evidence="ECO:0000255"
FT DOMAIN 598..750
FT /note="RNase NYN"
FT /evidence="ECO:0000255"
FT REGION 17..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 262..321
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 388..424
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 774..823
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 813..858
FT /note="CoCUN"
FT /evidence="ECO:0000250|UniProtKB:O75113"
FT COMPBIAS 20..37
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 283..319
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 388..417
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 774..790
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 791..814
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 429
FT /note="R -> P (in Ref. 2; AAH59205)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 858 AA; 97085 MW; 305089C2B532AFC7 CRC64;
MSTTRPLLGM KRITEVTCTE PPGGRQSPTA SRAQPDSLTV DEFTVHEDKQ TELKCSKPKV
EQVFQVTFTI IGLLDHTGAH GSKASRQIWL QLKGKKEDVY KAKEYVKGLC DPELQKEEWY
PVDMHCIFAG ARGLFLDRLL RDTSAEVQVL EPGRLKLSGC AEAVVMAQSR VQQFVALFQE
KRSLPADREP SVKRKFKTFV EDRADKYAME LLLLPSALKE ELLGLAQSPT QPIVIIDLEQ
DRSQTSTPVT DLSNRILDTT FEDKTSPITP EVMPGLNGRP CNKRRSSESE QRDTKRQYSL
ERREEEQCEE REREPTKTWT VKSAKGTLAA SEMTNESEAV SPETNLRCLV NFFRTMGYQQ
DVVERVVRET GQTEDTFLLL ERIVEETQKT QSTQGAQRTS RTPDPSPCAN ASSTSTSNRL
KEKERVQMRA LAEIKCKENI RPPSTNGIGQ KNQTSSVPLA SATLKRNNGA QTDLCEVIII
DDEEDFTETE RKPRLTPLDL KPESRFDYLP RGSSQTMVPV RMETVTNLRS SSQGPPLRTS
DTRPGCSYQT LPGRAPLPRS EAQYTSKAAP LTGMSRFQQS LRTPYRLILQ NEPGSPNLRH
IIIDGSNVAM AHGLHRVFSC RGIAIAVEAF WRRGHREITV FVPQWRQKKD PNITEQHFLN
QLENLRLLSF TPSREVCGHR ISSHDDRFLL HLAEKTGGVI VTNDNLRDFV SQSEAWRRII
HERLLQFTFV EDHFMIPDDP LGKHGPHLDE FLLKDSRGSP IIPPLRTDLR ATPSVYSQAA
QSTAHPSSPS HWPHSGPPDW HLPRPSPSPP PQRSPSETTE LKRKLYDIFP DQKQRIDRIL
SDNPYMRDLN ALSGLLLG
