ID   N4BP1_XENLA             Reviewed;         848 AA.
AC   Q7ZXG4;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=NEDD4-binding protein 1 {ECO:0000250|UniProtKB:Q6A037};
DE            Short=N4BP1 {ECO:0000250|UniProtKB:Q6A037};
DE            EC=3.1.-.- {ECO:0000250|UniProtKB:O75113};
GN   Name=n4bp1 {ECO:0000250|UniProtKB:Q6A037};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Potent suppressor of cytokine production that acts as a
CC       regulator of innate immune signaling and inflammation. Acts as a key
CC       negative regulator of select cytokine and chemokine responses elicited
CC       by TRIF-independent Toll-like receptors (TLRs), thereby limiting
CC       inflammatory cytokine responses to minor insults (By similarity). Has
CC       ribonuclease activity (By similarity). {ECO:0000250|UniProtKB:O75113,
CC       ECO:0000250|UniProtKB:Q6A037}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q6A037}. Nucleus {ECO:0000250|UniProtKB:Q6A037}.
CC       Nucleus, nucleolus {ECO:0000250|UniProtKB:Q6A037}. Nucleus, PML body
CC       {ECO:0000250|UniProtKB:Q6A037}. Note=Primarily localizes to the
CC       nucleolus. Also localizes to the PML nuclear bodies, when desumoylated.
CC       {ECO:0000250|UniProtKB:Q6A037}.
CC   -!- SIMILARITY: Belongs to the N4BP1 family. {ECO:0000305}.
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DR   EMBL; BC045006; AAH45006.1; -; mRNA.
DR   RefSeq; NP_001080872.1; NM_001087403.1.
DR   AlphaFoldDB; Q7ZXG4; -.
DR   SMR; Q7ZXG4; -.
DR   DNASU; 380566; -.
DR   GeneID; 380566; -.
DR   KEGG; xla:380566; -.
DR   CTD; 380566; -.
DR   Xenbase; XB-GENE-1216145; n4bp1.L.
DR   OrthoDB; 771251at2759; -.
DR   Proteomes; UP000186698; Chromosome 4L.
DR   Bgee; 380566; Expressed in blastula and 19 other tissues.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR   GO; GO:0016605; C:PML body; ISS:UniProtKB.
DR   GO; GO:0003729; F:mRNA binding; ISS:UniProtKB.
DR   GO; GO:0004540; F:ribonuclease activity; ISS:UniProtKB.
DR   GO; GO:0043130; F:ubiquitin binding; ISS:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0001818; P:negative regulation of cytokine production; ISS:UniProtKB.
DR   GO; GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR   GO; GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
DR   GO; GO:0045071; P:negative regulation of viral genome replication; ISS:UniProtKB.
DR   GO; GO:0045088; P:regulation of innate immune response; ISS:UniProtKB.
DR   InterPro; IPR036612; KH_dom_type_1_sf.
DR   InterPro; IPR021869; RNase_Zc3h12_NYN.
DR   Pfam; PF11977; RNase_Zc3h12a; 1.
DR   SUPFAM; SSF54791; SSF54791; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Immunity; Innate immunity; Nuclease; Nucleus;
KW   Reference proteome; RNA-binding.
FT   CHAIN           1..848
FT                   /note="NEDD4-binding protein 1"
FT                   /id="PRO_0000301988"
FT   DOMAIN          81..165
FT                   /note="KH-like"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          516..668
FT                   /note="RNase NYN"
FT                   /evidence="ECO:0000255"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          220..249
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          801..848
FT                   /note="CoCUN"
FT                   /evidence="ECO:0000250|UniProtKB:O75113"
FT   COMPBIAS        1..19
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        220..236
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   848 AA;  94915 MW;  4172FB5E8777EAE0 CRC64;
     MATGSVHSSS GNGRSQAATV VDEFTAPGDK KSLLERSRPR VQALFPVLFT VLGSLETFHQ
     SEVKEDGEKP CRIWLQLKGG KQAVGRAKEY LKGICEPEME EKQSYPKEMH CIFAGAQGSF
     LNHLIRDTYA NVTVPDIGVL SMKGGTEPVV MAQSRVQQFI RLFNDNVSLP NHKEPAVKKK
     FKLYVEKHAD KYTVDLLLLP SALKSELLSL ACDDQLSGED FSRQSSDDKV ESQVNQKDEV
     SRNGAGTPVT ELTSQLDSVF SSVAEDSPCL MERPYSEQDR LSVKRRSSEA EERFSKKPFS
     LEAVQVDGPV NRNADTNNVP IIDLISEPSD FEDSVILVEA DDNVSSETEY KILVNFFKTM
     GYSQNVVEKV IGDLGQSEEP LKLLEEIEKQ SKKELYSHPS SNSTELLKAR GTSNLPKCKI
     AIAEPVQQSA APLKTQCKAL LATDKAGPSN SSHDKSQTKA TVANTIPQDQ KTTIAPTERP
     LNKHSDPPLT GVQIFQNSLK VEYRLELKNE PGKLDLKHII IDGSNVAMSH GLKHFFSCRG
     IALAVEYFWN KGHRNITVFV PQWRTKRDPY ITEQHFLQQL QELGILSFTP SRTVLGARIA
     SHDDRFLLHL AERTGGIIVT NDNFREFVVE SPMWREIIKE RLLQYTFAGD IFMLPDDPLG
     RYGPKLDDFL SKQPINRTMH SSSPSSNEMF IPRDHFAPPR AMAPRNGLNQ PMTQRNGLSQ
     PMAPRTELNQ HMAPRTGLNQ HMAPRNALRQ PMAPKTGLSQ PIAPRTGLDE SSLFQLIKPQ
     EPFPPAVHNI ARPRPIIPPQ RSPSETMELR EALLKIFPET DQRHKINEIL SAHPYMRDLN
     ALSAMVLD