ID   N4BP1_XENTR             Reviewed;         819 AA.
AC   Q6DJS0;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=NEDD4-binding protein 1 {ECO:0000250|UniProtKB:Q6A037};
DE            Short=N4BP1 {ECO:0000250|UniProtKB:Q6A037};
DE            EC=3.1.-.- {ECO:0000250|UniProtKB:O75113};
GN   Name=n4bp1 {ECO:0000250|UniProtKB:Q6A037};
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Potent suppressor of cytokine production that acts as a
CC       regulator of innate immune signaling and inflammation. Acts as a key
CC       negative regulator of select cytokine and chemokine responses elicited
CC       by TRIF-independent Toll-like receptors (TLRs), thereby limiting
CC       inflammatory cytokine responses to minor insults (By similarity). Has
CC       ribonuclease activity (By similarity). {ECO:0000250|UniProtKB:O75113,
CC       ECO:0000250|UniProtKB:Q6A037}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q6A037}. Nucleus {ECO:0000250|UniProtKB:Q6A037}.
CC       Nucleus, nucleolus {ECO:0000250|UniProtKB:Q6A037}. Nucleus, PML body
CC       {ECO:0000250|UniProtKB:Q6A037}. Note=Primarily localizes to the
CC       nucleolus. Also localizes to the PML nuclear bodies, when desumoylated.
CC       {ECO:0000250|UniProtKB:Q6A037}.
CC   -!- SIMILARITY: Belongs to the N4BP1 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC075104; AAH75104.1; -; mRNA.
DR   RefSeq; NP_001004870.1; NM_001004870.1.
DR   AlphaFoldDB; Q6DJS0; -.
DR   SMR; Q6DJS0; -.
DR   PaxDb; Q6DJS0; -.
DR   PRIDE; Q6DJS0; -.
DR   DNASU; 448184; -.
DR   Ensembl; ENSXETT00000016323; ENSXETP00000016323; ENSXETG00000025007.
DR   GeneID; 448184; -.
DR   KEGG; xtr:448184; -.
DR   CTD; 9683; -.
DR   Xenbase; XB-GENE-1216138; n4bp1.
DR   eggNOG; KOG3777; Eukaryota.
DR   InParanoid; Q6DJS0; -.
DR   OMA; RIDRILC; -.
DR   OrthoDB; 771251at2759; -.
DR   PhylomeDB; Q6DJS0; -.
DR   TreeFam; TF315783; -.
DR   Proteomes; UP000008143; Chromosome 4.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000025007; Expressed in testis and 12 other tissues.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0016605; C:PML body; ISS:UniProtKB.
DR   GO; GO:0003729; F:mRNA binding; ISS:UniProtKB.
DR   GO; GO:0004540; F:ribonuclease activity; ISS:UniProtKB.
DR   GO; GO:0043130; F:ubiquitin binding; ISS:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0001818; P:negative regulation of cytokine production; ISS:UniProtKB.
DR   GO; GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR   GO; GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
DR   GO; GO:0045071; P:negative regulation of viral genome replication; ISS:UniProtKB.
DR   GO; GO:0045088; P:regulation of innate immune response; ISS:UniProtKB.
DR   InterPro; IPR021869; RNase_Zc3h12_NYN.
DR   Pfam; PF11977; RNase_Zc3h12a; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Immunity; Innate immunity; Nuclease; Nucleus;
KW   Reference proteome; RNA-binding.
FT   CHAIN           1..819
FT                   /note="NEDD4-binding protein 1"
FT                   /id="PRO_0000301989"
FT   DOMAIN          80..164
FT                   /note="KH-like"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          517..669
FT                   /note="RNase NYN"
FT                   /evidence="ECO:0000255"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          226..247
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          666..736
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          772..819
FT                   /note="CoCUN"
FT                   /evidence="ECO:0000250|UniProtKB:O75113"
FT   COMPBIAS        1..15
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        672..690
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   819 AA;  91760 MW;  1AAF44DBA52C4C1F CRC64;
     MASGSVQSSS GNGRRQAAVV DEFTVPGDKR SLLEKSRPRI QALFPVLFTV LGSLETFQQS
     EVKEDEEKPG RIWLQLKGEK QAVRRAKEYV KGICEPELEE KQSYPKEMHC IFAGAQSSFL
     NHLIQDTCAD VALSDIGVLG IKGATEPVVM AQSRVQQFIA LFKDNLSLPS HKEPAVKKKF
     KLYVEKHVDK YTVDLLLLPS ALKSELLSLA CDNQLSCEDL SRQSSDDKAE CKVNQKDEVS
     RKGAGTPVTE LTSQLDSVFS SAAEDSPCLI ECPYSEQDRL SVKRRSSEAE ERFSKKPFSL
     EAVQVDGPVN RNADKNNVPI IDLISEPSDF EDSVIVVEAD DCVSSETEYK ILVNFFKTMG
     YSQIVVEKVI DDLGQSEEPL KLLEEIEKQS KKELSVPFSQ SSSYSTDLLK ARGTSSLPKC
     KIAIAETVQS AAPSKVQPKA LLATDKAGPS NPFCYKSQTK ATVVNPIPTD QTTIVAPAER
     AFNNHFDPPL TGVQIFQNSL KVQYRLELKN EPGRWDLKHI IIDGSNVAMS HGLQRFFSCR
     GIALAVEYFW KKGHRNITVF VPQWRTKRDP FITEQHFLQQ LQELGILSFT PSRTVLGARI
     ASHDDRFLLH LAERTGGIII TNDNFREFVV ESPSWREIIK ERLLQYTFAG DIFMLPDDPL
     GRYGPKLDDF LSKQPNNRTV HSSFPSSNER FVPRDQFAPP RNMAPKTGLN QPMAPRAGHN
     QPMAPRAGHN QPMAPRTGLS QHRFLQLIKP QEPFTPALHN IARPCPIMPP ERSPSETMQL
     KEALLKIFPE ADQRHKINEI LTAHPFMRDL NALSAMVLD